tangina

Question 1

What is the most commonly used dye for routine histologic nuclear and cytoplasmic staining

Answer 1

hematoxylin

Question 2

What mordants are used with hematoxylin to demonstrate nuclear and cytoplasmic structures

Answer 2

alum and iron

Question 3

What color do aluminum salt lakes produce with hematoxylin

Answer 3

blue

Question 4

What color do ferric salt mordants produce with hematoxylin

Answer 4

blue-black

Question 5

What are examples of aluminum hematoxylin solutions

Answer 5

Mayer’s hematoxylin Ehrlich’s hematoxylin Cole’s hematoxylin Harris hematoxylin

Question 6

Which aluminum hematoxylin is suitable for acid decalcified tissues and tissues acidic from prolonged formalin storage

Answer 6

Ehrlich’s hematoxylin

Question 7

Which aluminum hematoxylin is widely used for routine nuclear staining and exfoliative cytology

Answer 7

Harris hematoxylin

Question 8

What are examples of iron hematoxylin solutions

Answer 8

Weigert’s hematoxylin Heidenhain’s hematoxylin

Question 9

What is routinely used as a counterstain after hematoxylin

Answer 9

eosin

Question 10

What is the most commonly used form of eosin

Answer 10

eosin Y (yellowish red acidic dye)

Question 11

What are other forms of eosin

Answer 11

eosin B and erythrosin B (bluish deeper red) ethyl eosin (eosin S)

Question 12

What stain is used for demonstration of connective tissue in Van Gieson stain

Answer 12

acid fuchsin with picric acid

Question 13

What fluorescent dye discriminates between dead and living tissues

Answer 13

acridine orange (green for DNA red for RNA)

Question 14

What stain demonstrates calcium salt deposits and phosphatase activity

Answer 14

acridine red 3B

Question 15

What stain is used for counterstaining epithelial sections

Answer 15

aniline blue

Question 16

What stain forms orange-red lake with calcium at pH 4.2 used to detect calcium in calcified tissues

Answer 16

alizarin red S

Question 17

What stain is more specific for connective tissue and epithelial mucin and stains acid mucopolysaccharides

Answer 17

alcian blue

Question 18

What stain is used for hemoglobin

Answer 18

benzidine

Question 19

What stain is used as contrast stain for Gram’s technique acid fast and Papanicolaou method and stains diphtheria organisms

Answer 19

Bismarck brown

Question 20

What stain is resistant to strong acid dyes and gives good nuclear definition with iron hematoxylin

Answer 20

celestine blue

Question 21

What stain is used for elastic tissues amyloid and myelin

Answer 21

Congo red

Question 22

What nuclear stain is used for amyloid in frozen sections and platelets in blood

Answer 22

crystal violet

Question 23

What stain colors nervous tissues violet especially Nissl bodies

Answer 23

cresyl violet

Question 24

What stain is used for blood leukocyte differentiation

Answer 24

Giemsa stain

Question 25

What metallic impregnation stain is made of gold chloride and mercuric chloride

Answer 25

gold sublimate

Question 26

What stain is the oldest used for microscopic study of starch granules amyloid cellulose carotenes and glycogen

Answer 26

iodine

Question 27

What iodine stain is used for microorganisms

Answer 27

Gram’s iodine

Question 28

What iodine stain is used as a test for glycogen amyloid and corpora amylacea

Answer 28

Lugol’s iodine

Question 29

What stain is used for demonstrating mitochondria in intravital staining

Answer 29

Janus green

Question 30

What weakly basic dye is used as contrast stain for Ascaris eggs erythrocytes and bacterial spores

Answer 30

malachite green

Question 31

What stain colors chromatin green in acidic conditions

Answer 31

methyl green

Question 32

What stain is valuable for plasma cells and cytological examination of fresh sputum and diagnosis of diphtheria

Answer 32

methylene blue

Question 33

What basic dye is recommended for observing cell granules and phagocytic vacuoles

Answer 33

neutral red

Question 34

What lipophilic stain accumulates in lipid globules staining them red

Answer 34

Nile red

Question 35

What stain substitutes for carbol fuchsin and metallic staining used in acid fast bacilli staining

Answer 35

Nile blue

Question 36

What stain is excellent for elastic fibers and used in dermatological studies

Answer 36

orcein

Question 37

What stain is used for microanatomical contrast and circulatory system demonstration by injection

Answer 37

Prussian blue

Question 38

What stain is used with osmic acid to fix and stain blood and granular tissues

Answer 38

rhodamine blue

Question 39

What stain is used in aqueous solution for identification of spirochetes reticulum and fiber stains

Answer 39

silver nitrate

Question 40

What dye is recommended for staining Nissl granules or chromophilic bodies

Answer 40

toluidine blue

Question 41

What dye is used for demonstration of neuroglia in frozen sections

Answer 41

victoria blue

Question 42

What dyes are used for demonstration of intracellular fats and are colored black orange and red

Answer 42

oil soluble dyes or lysochromes

Question 43

What is the most sensitive oil soluble dye that stains phospholipids and neutral fats

Answer 43

Sudan black B

Question 44

What is the first Sudan dye introduced into histochemistry and stains orange

Answer 44

Sudan III

Question 45

What Sudan dye stains triglycerides giving a deep intense red stain

Answer 45

Sudan IV

Question 46

What solvent should always be distilled unless otherwise stated for stains

Answer 46

water

Question 47

What solvent is used in various concentrations for stains

Answer 47

ethyl alcohol

Question 48

What solvent is an aqueous solution of saline added to hot distilled water

Answer 48

aniline water

Question 49

What solvent is used in aqueous solution at 0.5 to 5 percent for stains

Answer 49

phenol