Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Mycobacterium > tb_sunday > Flashcards

tb_sunday Flashcards

(85 cards)

Start Studying
1
Q

partially acid fast organisms that might give a false+ auramine stain

A

rhodococcus, nocardia, legionella, crypto cysts, isosproa, cyclospora, microsporidia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

number of fields that must be read on ZN smear

A

300

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Mycobacti organisms that show cording?

A

M. tb & M. kansasii

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Name organism that is a scotochromogen at 37C but photochromogen at 25C?

A

M. szulgai

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Describe handling of gastric specimens?

A

process in 4h, or add 100mg sodium carbonate to neutralize acidity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

List unsat specimens

A

pooled sputum or urine, nonsterile containers, waxy containers or dry swabs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Reagents used for digestion of normal flora?

A

NALC, NaOH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Mucolytic agent

A

N-acetyl-L-cysteine, digests sputum and allows NaOH to be used a lower concentration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Decontaminating agent

A

4% NaOH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Purpose of sodium citrate

A

binds heavy metals present in specimens to inactivate acetylcysteine.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Decontaminating agent for Pseudomonas

A

%5 oxalic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Holding time for LJ slants?

A

37^C, 6-8w

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Mycobacteria characteristics

A

not readily gram-stained, nonmotile rods, high lipid content, hydrophobic surface

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Differentiate M. tb from M. bovis

A

M.bovis: susceptible to T2H & niacin -, pza-, nitrate-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

NTMS?

A

photo, scoto, nonphoto, rapid growers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Genera containing mycolic acid

A

Corynebacterium, Gordona, Mycobacterium, Nocardia, Rhodococcus, Tsukamurella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

acid-fastness describes which characteristic?

A

waxy envelope and presence of mycolic acid (lipid)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Culture used for M. leprae

A

none. Alright, armadillos and can be injected into footpads of mice.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Saprophytic mycobacterium

A

M. gordonae, M. pheli, smegmatis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Media growth for semi-quantitative catalase test?

A

LJ

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

mycobacti distinguised by salt tolerance?

A

rapid growers, fortuitum, chelonae, flavescens++, trivale

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

media growth for niacin and nitrate test and age requirement of culture.

A

LJ nitrate : 3-5 wLj niacin: 18-28 day growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

MTB organisms

A

tb, bovis, africanum, microti, canetti

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

HPLC IDs mycobacti based on which characteristic?

A

mycolic acids in cell wall

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Differentiate M. simiae from MTB complex?
M. simiae: SQ cat+, pigment+, catalae+ at 68^C, nitrate-, accuprobe and NAAT
27
Rate of air flow in BSC?
70% recirculated air, inward face velocity of 75ft/min
28
BacTec 960 MGIT detects growth by which method?
reduced oxygen produces fluorescence due to organism growth.
29
Incubation temperature for skin-specific mycobacti
30C (marinum, ulcerans, haemophilum)
30
RFLP enzyme?
restriction endonuclease
31
procedure used to reduce tb exposure?
open safety cups under BSC
32
sensitivity of PCR using smear+ organism
>95%
33
genera that contain mycolic acid
mycobacteria, rhodococcus, nocardia
34
Partially acid fast organisms
rhodococcus, nocardia, gordona
35
organisms with thin braching filaments
nocardia
36
Cell component used to identify mycobacti using HPLC?
mycolic acid
37
length of time required to hold negative organisms
6-8w,+4 if smear+
38
Biochems for M. tb
niacin, nitrate, citrate
39
TB positive for growth on MAC without crystal violet?
group 4 rapid growers
40
biochems used to differentiate 2 chelonae subspp. & M. kansasii from M. fortuitum?
5% NaCl
41
niacin test summary
4 w old LJ culture+cyanogen bromide &amine=yellow (+)
42
SQ catalase test summary
2w LJ (NOT BAP) culture+Tw80&30H2O2, measure height of bubbles (>45mm ,45mm)
43
>45mm SQ catalase organisms
M. kansasii, simiae, scotochromogens, nonphotochromogenic saprophytes
44
growth on MAC without crystal violet
m. fortuitum and m. phlei
45
QC+ for heat-stable catalase test
M. kansasii+
46
iron uptake organisms
m. fortuitum+, chelonae-
47
tellurite reduction test
MAC+, black precipitate
48
nitrate reduction organisms+
mtb, ks, sz, sm, fo
49
mycobacteria tuberculosis characteristics:
slow growth, nonphoto, nude color dry crumbly morphology, nitrate+, niacin+, catalase 68-
50
M. xenopi characteristics in culture
hot water, G at 42^C, 10x magnifaction show birds nest-like growth
51
mycobacti responsible for AIDS patients
MAC
52
3 mycobacti that grow at 30^C
haemophilum, ulcerans, marinum HUM
53
ABCs for mycobacti
streptomycin, isoniazid, rifampin, ethambutol, pyrazinamide
54
Optimal growth temperature for rapid growers?
28^C
55
GenProbe Accuprobe: type of reading and organisms tested?
chemiluminisecence/ M. tb, MAC, M. gord, M. kan, cocci
56
BacTec MGIT PANTA
polymyxin B, amphotericin b, nalidixic acid, trimethoprim, azolcillin
57
pigment in mycobacti?
carotenoids
58
MGIT growth supplement
albumin-protective agent to bind free fatty acid, dextrose is energy source, catalsase destroys toxic peroxides in media, oleic acid involved in metabolism
59
MGIT growth explanation
oxygen sensitive fluorescent compound suppressed by high oxygen concentration dissolved in 7H9 broth. As bacteria grow and consume oxygen, suppression released and fluorescence detected.
60
NaCl 5% growth
flavescens and triviale
61
describe accuprobe (genprobe) assay
tubes labeled with chemiluminiscent ssDNA probe complementary to rRNA of target organism forming DNA_RNA hybrid.
62
Name acceptable tb specimens
Sputum: 3 separate samples, from am collections. 5 ml/each.Urine: 2 ml, am samples, transport to lab in 30 minBlood: 10ml use DuPont isolator tube at RTGastric: 5-10 Ml from fasting patient, transport in 4h or add sodium carbonateStool: sterile container, only AIDS patients
63
Tb SOC
Early am sputum or aerosol collection
64
Why unsat pooled specimens?
1. Contamination increased | 2. Positive sample may be diluted by negative sample
65
Compounds used to neutralize gastric lavage specimens
Sodium carbonate 10% tri sodium phosphate Crystalline di sodium phosphate
66
3 Anticoagulants that don't inhibit acid fast bacilli
CitrateHeparinAmmonium oxalate
67
+\- for 68 catalase test
+M. fortuitum, -M tuberculosis
68
3 staining procedures for acid fast bacilli
ZnKinyounRhodamone-aura mine
69
Hazards of cyanogen bromide (niacin test)
Volatile and toxic Gives off cyanide gas Impure CB decomposes and explodes
70
Suitable disinfectants for tb?
70% ethyl alcohol Phenolic soap Sodium hypochlorite Formaldehyde 3-8%
71
Preferred media for isoniazid resistant m tb?
Middle brook 7h11 because it contains casein hydrolysate to improve strain reccoery
72
Unsuitable disinfectant for tb?
Quaternary ammonium compounds
73
Requirements to identify mtb
Cellular and colonial characteristics, rate of growth, niacin, nitrate, 68 catalase-
74
3 Sputum methods reagents acceptable for recovery of m tb
NalcNaoh Zephiran tri sodium phosphate
75
Describe xenopi culture characteristics
Slow growth Circular colonies Filamentous extensions on cornmeal agar
76
Mycobacterium cutaneous lesions secondary to post surgical wound infections, trauma or needle injections caused by:
M fortuitum chelonae complex
77
Recovery of m marinum and m ulcer ans enhanced by
Incubation at 30c
78
Tellurite reduction test positive for
Rapid growers and Mac
79
Factors affecting photochromogenicity
Oxygen Age of culture Exposure to light1 hr Crowding in culture
80
3 d arylsulfatase positive organisms
Xenopi (@42C) and Fortuitum
81
BACTEC 460TB media used in machine
12B vials use 7H12 broth. 7h12 broth has ABCs and c14 labeled palmitic acid
82
M bovis biochemical tests
Niacin weak/negative Nitrate reduction Catalase inactivated at 68 T2H sensitivity
83
Bactec MGIT 960 and BACTEC 460TB advantages over conventional media
Reduced time from 19 d to 8d. Bactec used for susceptibility testingAutomated monitorLarge inoculum and liquid media increase sensitivity
84
BACTEC 460TB GI positive reads?
10
85
BACTEC 460TB disadvantages?
Radioactive material Expensive inst reqd Not possible to examine colonial morphology Possible cross contamination if needle heater fails
86
Tween hydrolysis test helpful for identification of:
Scotochromogenic and nonphotochromigenic

Mycobacterium (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions