Flashcards in Test 1 Deck (22)
What is serum?
Liquid portion of blood, same as plasma but with no fibrinogen
Taken with tubes containing no anticoagulant
What are the granulocytes and what colour do they stain?
Neutrophils- small pink granules (phagocytic to kill bacteria)
Basophils- large blue/black granulocytes (allergies/histamine)
Eosinophils- large orange granules- parasitic infections
What are the agranulocytes and what do they do?
Lymphocytes- immune response
What is the red tube? What are it's common uses?
Tube with no additive
Commonly used for chemistry tests, must allow 10 mins for clot to form
Used to acquire serum
What is plasma?
Liquid portion of unclotted blood- electrolytes (can't use sodium tubes), protein
Taken with anticoagulant tubes
What are the two types of EDTA tubes? What's the biggest difference?
EDTA-K3: liquid. Can dilute draw if not full, falsely lowering results. 0.1ml/1ml blood
EDTA-K2: powder. 1.8mg/1ml blood
Both bing calcium
What are EDTA tubes? What are they used for?
Good for collecting whole blood- routine hematology tests
Blood can be used for cellular morphology for 4 hrs (if longer, neutrophils swell and RBCs change shape)
Platelets for up to 24 hours
Must mix well for 5 mins
What does excess EDTA do?
Causes platelets to swell and burst
What are the blue and black tubes? What is the difference?
Black: 1:4 ratio, ESR test, cannot be conducted if even small amounts of clotting occur
Blue: 1:9 ratio, coagulation tests, MUST BE FULL DRAW
Both bind calcium
What is the specimen requirement correlated with purple tubes?
EDTA whole blood
What is the specimen requirement correlated with blue tubes?
Citrated plasma- for aliquoting
What is the specimen requirement correlated with black tubes?
Citrated whole blood- for ESR
What is the specimen requirement correlated with green tubes?
Heparinized plasma (aliquoting for electrolytes)
Heparinized whole blood (for enzymes)
What is the specimen requirement correlated with grey tubes?
What are some key features of heparin tubes?
ONLY tube to use antithrombin to prevent clotting
Used to test for electrolytes and RBC tests
Not used for hematology because it stains background
Preserves cells for up to 24hrs
What are the different types of heparin additives?
Sodium (can't be used for electrolyte collection)
What are some key features of fluoride tubes?
Must have 90% draw
Good for delayed glucose tests b/c prevents autolysis
What happens if the tourniquet is left on too long?
Hemoconcentration occurs, pressure causes plasma to leave veins, skewing ratio.
Increased hemoglobin and hematocrit
What is the most commonly used phlebotomy needle?
What are the three components of a needle?
Hub, shaft, and bevel
What is the average length of a phlebotomy needle?