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Flashcards in Test 1 Deck (22)
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1

What is serum?

Liquid portion of blood, same as plasma but with no fibrinogen

Taken with tubes containing no anticoagulant

2

What are the granulocytes and what colour do they stain?

Neutrophils- small pink granules (phagocytic to kill bacteria)

Basophils- large blue/black granulocytes (allergies/histamine)

Eosinophils- large orange granules- parasitic infections

3

What are the agranulocytes and what do they do?

Lymphocytes- immune response

Monocytes- phagocytic

4

What is the red tube? What are it's common uses?

Tube with no additive

Commonly used for chemistry tests, must allow 10 mins for clot to form

Used to acquire serum

5

What is plasma?

Liquid portion of unclotted blood- electrolytes (can't use sodium tubes), protein

Taken with anticoagulant tubes

6

What are the two types of EDTA tubes? What's the biggest difference?

EDTA-K3: liquid. Can dilute draw if not full, falsely lowering results. 0.1ml/1ml blood

EDTA-K2: powder. 1.8mg/1ml blood

Both bing calcium

7

What are EDTA tubes? What are they used for?

Good for collecting whole blood- routine hematology tests

Blood can be used for cellular morphology for 4 hrs (if longer, neutrophils swell and RBCs change shape)

Platelets for up to 24 hours

Must mix well for 5 mins

8

What does excess EDTA do?

Causes platelets to swell and burst

9

What are the blue and black tubes? What is the difference?

Sodium citrate

Black: 1:4 ratio, ESR test, cannot be conducted if even small amounts of clotting occur

Blue: 1:9 ratio, coagulation tests, MUST BE FULL DRAW

Both bind calcium

10

What is the specimen requirement correlated with purple tubes?

EDTA whole blood

11

What is the specimen requirement correlated with blue tubes?

Citrated plasma- for aliquoting

12

What is the specimen requirement correlated with black tubes?

Citrated whole blood- for ESR

Mix well

13

What is the specimen requirement correlated with green tubes?

Heparinized plasma (aliquoting for electrolytes)

Heparinized whole blood (for enzymes)

14

What is the specimen requirement correlated with grey tubes?

Plasma- aliquot

15

What are some key features of heparin tubes?

ONLY tube to use antithrombin to prevent clotting

Used to test for electrolytes and RBC tests

Not used for hematology because it stains background

Preserves cells for up to 24hrs

1:5





16

What are the different types of heparin additives?

Ammonium

Lithium

Sodium (can't be used for electrolyte collection)

17

What are some key features of fluoride tubes?

Grey

Must have 90% draw

Good for delayed glucose tests b/c prevents autolysis

18

What happens if the tourniquet is left on too long?

Hemoconcentration occurs, pressure causes plasma to leave veins, skewing ratio.

Increased hemoglobin and hematocrit

19

What is the most commonly used phlebotomy needle?

21 gauge

20

What are the three components of a needle?

Hub, shaft, and bevel

21

What is the average length of a phlebotomy needle?

1-2"

22

What are the three components of the evacuated tube system?

Stoppered tube

A needle

Baruta inter holder