Flashcards in Test 1 : (Quizzes 1 -3) Deck (47):
what is not a function of the RNA processing steps?
the 3' poly A tail additions enhances transcription
What are the RNA processing steps?
- 5' methyl cap, facilitates the attachment of the ribosomes
- protect mRNA from degradation by the ribonuclease
- contribute to the stability of the mRNAs
What promotes the initiation of DNA replication in bacteria?
-increase in bacterial size
- high levels of DNAa to melt dsDNA at the oriC
- high ADP:ATP ratio
What is true of gram + bacteria
- stain purple
- have a much thicker peptidoglycan layer
-have one cell membrane
clostridium, lactobacilius and strep are all what gram stains?
Scientist that showed the the DNA : the amount of adenine = thymine
Experiment created by who:: used E.Coli and bacteriophage showed that DNA with radioactive labels ended up inside the bacterial cells?
Hershey and Chase
Came up with the experiment of the Rat being able to live when it was injected heat activated S cells?
Who proposed that DNA is semiconservative ?
Avery, McLeod and McCarthy
is the lagging strand discontinuous?
Where are the okazaki fragments formed ?
in the lagging stand
in which stand is DNA synthesis initiated from multiple primers ?
antibiotics with 2 antimicrobial actions target DNA replication?
What are the five subunits that make up the bacterial core RNA polymerase?
(sigma subunits::not sure Question 8 on quiz 1 )
what are the two transcription termination of the bacterial chromosome?
hair-pin loop termination
What unwinds the double stranded DNA helix ahead of the replication fork?
What enzyme makes up the phosphodiester bond between the 3'OH end of the newly completed okazaki fragments and the 5' po4 end of the adj okazaki fragment?
(Pentose Sugar, not sure Q11 on quiz 1)
a gene that codes for the same amino acid without having to change the nts is known as what?
What does it mean if a codon has a "Wobble"
occurs in the third position of the codon
silent mutation is also known as?
23sRNA is in charge of what?
catalyzes the large ribosomal subunit, this occurs in the formation of the peptide bond between the assembled peptide in the tRAN on the P site, an the amino acid on the A site
What protein acts as a translocase, in shifting the tRNA from the A site to the P site?
IF3 helps bind the small ribosomal sub unit _______and keeps the the large _____unit the translation initiation complex is fully assembled?
The operon contains the DNA sequence that is transcribed into polycistronic mRNA ( T or F)
what type of effect is occurs when translation of down stream gene is reduced or non-existent when an upstream gene fails to be translated?
what type of mRNA carries multiple genes that are translated into 2 or more protein?
What happens when translation coupling is occuring?
in polycis mRNA is when translation of a 5' gene is required for translation of downstream gene
what is negative inducible regulation
the regulation of expression of the araBAD operon by AraC in the presence of arabinose
trp-attenuation happens when there is an abundant of charged trp-tRNA ( T or F )
what is required for attenuation to take place?
blue/white screening , the blue colonies indicate the successful translation of the enzyme ________
the blue colonies acts on the chromogenic substrate
mRAN repressed further translation of the polycistron that codes for R protein ?
when the rRNA is low
ppGpp does what?
compete with NTP leading to inhibition of the RNA polymerase
RpoS degradation rates and translation rates are what types of determinants?
response to entry to stationary phase or environmental stresses
ompF, is the major porin protein whose levels are ______when E.coli are subject to low _______
protein that is not a major player in the response to periplasm accumulation of misfolded protein
EF2 does what?
ADP ribosylated and inactivated and inactivated by Diphtheria toxin
DNA-K binds to the sigma factor ______and targets it for degradation during normal temperatures, while during heat shock, it preferentially binds to _______protein?
what is the sensor in signal transduction
what is the response regulator that activates transduction of the operon involved in glutamine synthesis?
serves to protect pathogens from harsh environment, can be found in the assemblies of microbial cells
liquid chromatography mass spectrometry
reporter genes that are commonly used to make transcription fusion
lac Z , lac yY
two antibiotics that interfere with translation?
in prokaryotes, AUG is chosen based on