Test 1 : (Quizzes 1 -3) Flashcards Preview

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Flashcards in Test 1 : (Quizzes 1 -3) Deck (47):
1

what is not a function of the RNA processing steps?

the 3' poly A tail additions enhances transcription

2

What are the RNA processing steps?

- 5' methyl cap, facilitates the attachment of the ribosomes
- protect mRNA from degradation by the ribonuclease
- contribute to the stability of the mRNAs

3

What promotes the initiation of DNA replication in bacteria?

-increase in bacterial size
- high levels of DNAa to melt dsDNA at the oriC
- high ADP:ATP ratio

4

What is true of gram + bacteria

- stain purple
- have a much thicker peptidoglycan layer
-have one cell membrane

5

clostridium, lactobacilius and strep are all what gram stains?

Gram +

6

Scientist that showed the the DNA : the amount of adenine = thymine

chargaff

7

Experiment created by who:: used E.Coli and bacteriophage showed that DNA with radioactive labels ended up inside the bacterial cells?

Hershey and Chase

8

Came up with the experiment of the Rat being able to live when it was injected heat activated S cells?

Griffith

9

Who proposed that DNA is semiconservative ?

Avery, McLeod and McCarthy

10

is the lagging strand discontinuous?

Yes

11

Where are the okazaki fragments formed ?

in the lagging stand

12

in which stand is DNA synthesis initiated from multiple primers ?

Lagging

13

antibiotics with 2 antimicrobial actions target DNA replication?

Actinomycin D
Rafampin

14

What are the five subunits that make up the bacterial core RNA polymerase?

(sigma subunits::not sure Question 8 on quiz 1 )

15

what are the two transcription termination of the bacterial chromosome?

Rho-dependent termination,
hair-pin loop termination

16

What unwinds the double stranded DNA helix ahead of the replication fork?

helicase

17

What enzyme makes up the phosphodiester bond between the 3'OH end of the newly completed okazaki fragments and the 5' po4 end of the adj okazaki fragment?

(Pentose Sugar, not sure Q11 on quiz 1)

18

a gene that codes for the same amino acid without having to change the nts is known as what?

universal

19

What does it mean if a codon has a "Wobble"

occurs in the third position of the codon

20

silent mutation is also known as?

redundant

21

23sRNA is in charge of what?

catalyzes the large ribosomal subunit, this occurs in the formation of the peptide bond between the assembled peptide in the tRAN on the P site, an the amino acid on the A site

22

What protein acts as a translocase, in shifting the tRNA from the A site to the P site?

EF-G

23

IF3 helps bind the small ribosomal sub unit _______and keeps the the large _____unit the translation initiation complex is fully assembled?

SU ,SU

24

The operon contains the DNA sequence that is transcribed into polycistronic mRNA ( T or F)

true

25

what type of effect is occurs when translation of down stream gene is reduced or non-existent when an upstream gene fails to be translated?

Polar effect

26

what type of mRNA carries multiple genes that are translated into 2 or more protein?

polycistronic mRNA

27

What happens when translation coupling is occuring?

in polycis mRNA is when translation of a 5' gene is required for translation of downstream gene

28

what is negative inducible regulation

the regulation of expression of the araBAD operon by AraC in the presence of arabinose

29

trp-attenuation happens when there is an abundant of charged trp-tRNA ( T or F )

true

30

what is required for attenuation to take place?

translation initiation

31

blue/white screening , the blue colonies indicate the successful translation of the enzyme ________

beta-galactosidase

32

the blue colonies acts on the chromogenic substrate

X-gal

33

mRAN repressed further translation of the polycistron that codes for R protein ?

when the rRNA is low

34

ppGpp does what?

compete with NTP leading to inhibition of the RNA polymerase

35

RpoS degradation rates and translation rates are what types of determinants?

response to entry to stationary phase or environmental stresses

36

ompF, is the major porin protein whose levels are ______when E.coli are subject to low _______

increased, osmolarity

37

protein that is not a major player in the response to periplasm accumulation of misfolded protein

cyaA

38

EF2 does what?

ADP ribosylated and inactivated and inactivated by Diphtheria toxin

39

DNA-K binds to the sigma factor ______and targets it for degradation during normal temperatures, while during heat shock, it preferentially binds to _______protein?

RpoH, misfolded

40

what is the sensor in signal transduction

ntrB

41

what is the response regulator that activates transduction of the operon involved in glutamine synthesis?

ntrC

42

serves to protect pathogens from harsh environment, can be found in the assemblies of microbial cells

biofilm

43

LCMS

liquid chromatography mass spectrometry

44

reporter genes that are commonly used to make transcription fusion

lac Z , lac yY

45

two antibiotics that interfere with translation?

tetracycline, pyromicine

46

in prokaryotes, AUG is chosen based on

shine-dalgarno

47

in eukaryotes, downstream of the 5' cap is found within the consensus is where the AUG can be found

kozak sequence