Flashcards in Test 2 Deck (22)
Write the central dogma
DNA (replication;DNA polymerase)--> RNA (transcription; RNA polymerase)-> protein (translation; ribosome)
Requirements for DNA replication
Primer, DNA polymerase, dATP, dTTP, dCTP, and dGTP
What will be required for PCR?
Unique feature of A-DNA, B-DNA, and Z-DNA?
A- wide; anti formation; right handed
B- intermediate; anti; right handed
Z- skinny; anti + syn; left handed
Chemical forces that contribute to form double helical DNA
H+ and van der Waals between bases (base stacking)
Define Tm value
The temp at which half the helical structure is lost. The determination is observed at 260nm
What is required for reverse transcription?
Gene expression is?
The flow of genetic info from DNA to protein
What is promoter?
Specifically bind RNA polymerase and determine where transcription begins.
Common promoter regions found in bacterial genes
Eukaryotic- -75, -25
Prokaryotic- -35, -10
Common promoter regions founds in eukaryotic cell genes?
CAAT + TATAA boxes
What is shine-dalgarno sequence?
Ribosome binding site
Ribosome composition + function
Protein + rRNA
rRNA binds to shine-dalg
What is the first AA in bacterial proteins?
To remove non-coding region (introns)
What are restriction enzymes? (Palindrome site?)
Precise molecular scalpels that allow an investigator to manipulate DNA segments.
5' ATCGAT 3'
3' TAGCTA 5'
Polymerase Chain Reaction
Necessary components of PCR?
DNA polymerase, Taq, PFU
Enzyme to ligate fragments
Multicloning site with restriction enzyme sites
Difference between cloning vector and expression vector?
Cloning- specific for cloning
Expression- specific for gene expression