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Flashcards in Test 2 Deck (22)
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1

Write the central dogma

DNA (replication;DNA polymerase)--> RNA (transcription; RNA polymerase)-> protein (translation; ribosome)

2

Requirements for DNA replication

Primer, DNA polymerase, dATP, dTTP, dCTP, and dGTP

3

What will be required for PCR?

Buffer

4

Unique feature of A-DNA, B-DNA, and Z-DNA?

A- wide; anti formation; right handed
B- intermediate; anti; right handed
Z- skinny; anti + syn; left handed

5

Chemical forces that contribute to form double helical DNA

H+ and van der Waals between bases (base stacking)

6

Define Tm value

The temp at which half the helical structure is lost. The determination is observed at 260nm

7

What is required for reverse transcription?

Reverse transcriptase

8

Gene expression is?

The flow of genetic info from DNA to protein

9

What is promoter?

Specifically bind RNA polymerase and determine where transcription begins.

10

Common promoter regions found in bacterial genes

Eukaryotic- -75, -25
Prokaryotic- -35, -10

11

Common promoter regions founds in eukaryotic cell genes?

CAAT + TATAA boxes

12

What is shine-dalgarno sequence?

Ribosome binding site

13

Ribosome composition + function

Protein + rRNA
rRNA binds to shine-dalg

14

What is the first AA in bacterial proteins?

f methionine

15

Splicing

To remove non-coding region (introns)

----}GU----AG{-----

16

What are restriction enzymes? (Palindrome site?)

Precise molecular scalpels that allow an investigator to manipulate DNA segments.

5' ATCGAT 3'
3' TAGCTA 5'

17

PCR

Polymerase Chain Reaction

18

Necessary components of PCR?

DNA polymerase, Taq, PFU

19

Ligase

Enzyme to ligate fragments

20

Polylinker site

Multicloning site with restriction enzyme sites

21

Difference between cloning vector and expression vector?

Cloning- specific for cloning

Expression- specific for gene expression

22

What is cDNA?

Complementary DNA; comes from RNA by reverse transcriptase