Test #3 Flashcards

(75 cards)

0
Q

Metabolism includes reactions that breakdown complex molecules into less complex molecules, releasing energy

A

This category of reactions is termed at CATABOLISM

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1
Q

Metabolism includes reactions that use energy to make more complex molecules

A

This category of reactions is termed as ANABOLISM

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2
Q

Why does a bacterium need enzymes to break down glucose when the breakdown releases energy anyways?

A

Needed to reduce activation energy to make it happen at a faster rate

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3
Q

What affects does an enzyme have on the overall release of energy in an energy producing reaction?

A

The enzyme does not affect overall energy, only activation energy

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4
Q

Your critical bacterial culture is exposed to a toxin that acts as a competitive inhibitor of an essential reaction. What can you do to rescue your bacteria?
(You cannot remove the toxin)

A

Increase the amount of substrate in the reaction

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5
Q

______ is the total of all chemical reactions in the cell and is divided into two parts.

A

Metabolism

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6
Q

The breakdown of large molecules for energy

A

Catabolism

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7
Q

When smaller pieces are combined to make larger ones

A

Anabolism

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8
Q

Catabolism are fueling reactions that generate precursors for biosynthesis from ______-_______ reactions that provide ________ sources.

A

Energy conserving

Electrons

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9
Q

The synthesis of complex organic molecules from simpler ones and requires ________ from _______ reactions.

A

Energy

Catabolic

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10
Q

Each step of metabolism requires an ________ which are specifically made for each reaction

A

Enzyme

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11
Q

_______ are needed to carry out reactions at physiological conditions so they proceed in a timely manner.

A

Enzymes

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12
Q

Enzymes speed up the _______ at which a reaction proceeds towards ________.

A

Rate

Equilibrium

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13
Q

How are enzymes used to overcome constraints in the body?

A

They lower the activation energy which is important because the cells in the body cannot be heated, metals and salt concentrations cannot be changed.

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14
Q

Substrates have ______ energy than products

A

More

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15
Q

The energy required to form transition-state complex

A

Activation energy

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16
Q

How do enzymes speed up the reaction?

A

They lower the activation energy

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17
Q

Overall the reaction to form the transition state complex gives off energy but is not _________.

A

Spontaneous

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18
Q

What happens when enzymes bind to their substrates?

A

They change their shape, which changes their energy, which changes their functions & facilitates chemical reactions.

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19
Q

Enzyme activity is significantly affected by…..

A

pH
Temperature
Substrate concentration

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20
Q

How does substrate concentration affect the enzyme activity?

A

They are “bumping” into each other

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21
Q

What does changes in pH and temperature do to enzyme activity?

A

Changes the shape without denaturing and affects the ability for the enzyme to do its job.

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22
Q

What happens when you increase substrate concentration

A

It increases the rate of reaction by decreasing activation energy which starts a reaction sooner.

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23
Q

Why is body temperature regulated?

A

To facilitate enzyme actions

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24
What are the types of enzyme inhibition?
Competitive inhibition | Non-competitive (allosteric) inhibition
25
_____ _______ directly competes with the binding of substrates to the active site
Competitive inhibitor
26
Competitive inhibitors decrease function because of the ________ of inhibitor to substrate
Ratio
27
________ substrate can overcome inhibitor effect
Increase
28
What is needed for enzymes to function in competitive inhibition?
They must have their substrates in the active site
29
_______ ________ binds enzymes at sites other than the active site
Non-competitive (allosteric) inhibitor
30
How do allosteric inhibitors function?
They bind to different places than the substrate because the enzymes shake has been changed making it less active.
31
______ ______ consists of mostly regulatory enzymes whose activity is altered by small molecules
Allosteric regulation (positive or negative).
32
The allosteric effector binds to _______ at regulatory sites?
Non-covalently
33
The allosteric effector changes ________ of the enzyme and alters activity of _______
Shape | Catalytic site
34
The positive allosteric effector ______ _____ activity
Increases enzyme
35
The negative allosteric effector ________ the enzyme
Inhibits
36
In allosteric regulation, the __________ of inhibitor effects function
Concentration
37
What if the allosteric effector binds to the enzyme and never let's go? What is an example?
You have a poison enzyme that'll never work again. Ex: penicillin cannot make the cell wall because more enzyme cannot be made since the allosteric effector is constantly bound to the enzyme.
38
Thomas chech and Sidney Altman discovered that some _______ molecules can also _______ reactions
RNA | Catalyze
39
Enzymes made of RNA
Ribozymes
40
What are examples of ribozymes use?
Self splicing Catalyzing peptides bond formation And involved In self replication
41
_______ reactions are both catabolic and anabolic
Amphibolic
42
________ is needed for energy
ATP
43
Why does ATP have more energy than ADP?
There's more phosphates and phosphates contribute energy
44
What contains the energy in ATP?
Phosphate bonds with electrons
45
Where is the electron transport chain found?
It's found in membranes, examples include: mitochondrial inner membrane, chloroplasts, and cell membranes of prokaryotes
46
As electrons pass through the etc to the final electron acceptor, a __________ __________ ____________ is generated and used to synthesize ATP.
Proton motive force
47
Simply defined as the moving of hydrogen used to make ATP
Proton motive force
48
The final electron acceptor is oxygen
Aerobic respiration
49
The final electron acceptor is not pure oxygen, Ex: SO4 CO2
Anaerobic respiration
50
What it's the primary producer of ATP?
Oxidative phosphorylation
51
What are three types of chemoorganic fueling process ?
Aerobic respiration Anaerobic respiration Fermentation
52
Does aerobic or anaerobic respiration produce more ATP?
Aerobic
53
Energy differences in ______ respiration allows for more energy release from O2
Aerobic respiration
54
Which chemoorganic fueling process does not use the electron transport chain or protons time force
Fermentation
55
Fermentation uses a _______ electron acceptor
Endogenous
56
What is an endogenous electron acceptor?
Made by the cell already, and self contained; usually an intermediate of a pathway used to oxidize the organic energy source
57
In fermentation how is ATP synthesized?
Substrate level phosphorylation
58
The ____ ____ ___ utilizes movement of electrons from high to low energy which can be captured.
Electron transport chain
59
The first Electron carriers in the ETC have the most _______ activation energy
Negative
60
Since the first electron carrier is the most negative it results in the potential energy stored in the first redox couple to be _______ and. Used to form ATP
Released
61
The first electron carrier in the ETC is ______ and electrons are moved tot he next carrier and so on
Reduced
62
The Moving of electrons from molecule to molecule
ETC
63
The. Electron transport chain of mitochondria is located in?
The plasma membrane
64
Bacteria and archaea's ETC resembles mitochondrial ETC in some ways. How do they differ?
Have different electron carriers Maybe branched Maybe shorter May have lower phosphorous to oxygen level.
65
The ETC of e. Coil is a _______ pathway
Branched
66
The upper branch in the ETC of e. Coil has _______ phase and _____ aeration
Stationary | Low
67
The lower branch of ETC of e. Coil has ______ phase nd _____ aeration.
Log | High
68
The ETC of e. Coil has different aray of ______ used than in mitochondrial ETC
Cytochrome
69
For the etc to be branched it must have a _______
Periplasmic space
70
Given the apt synthase shown, write the stage of each beta subunit after one complete revolution of the gamma subunit relative to beta. (Write them next to each subunit shown)
ATP will form and a full turn occurs.
71
A bacteria is an obligate anaerobes. What type of energy production does this bacteria use?
Aerobic respiration
72
Give an example of a mechanism of gene regulation in a prokaryote that is not found in eukaryotes and explain why it is only possible in prokaryotes
Translation/transcription must occur simultaneously in prokaryotes and in eukaryotes it's at different times.
73
In the trp operon, is the repress or bound to DNA or floating free in the presence of tryptophan?
Repress or is bound to DNA when trp is present and prevents polymerase from activating transcription.
74
What would happen to the regulation of the trp operon if the operator was missing?
The regulator could never bind and turn off transcription -> regulation would have to solely rely on attenuation.