Test 3 Flashcards
(16 cards)
Methylation of DNA in eukaryotes
prevents transcription factor binding and decreases transcription
Acetylation of histones in eukaryotes
Decreases histone positive charge and increases transcription
microRNAs
repress translation of target mRNAs by binding to their 3’UTRs
Which human cell contains a gene that specifies eye color
All cells contain the gene that specifies eye color
The regions of chromosomes that form heterochromatin
contain few genes
Comparing the ground state of transcription in prokaryotes vs eukaryotes
The default ground state of transcription in prokaryotes is ON and low or basal
When a cloned gene is inserted into a noncritical site in the mouse genome by homologous recombination, the result is
Knock in
Ubiquitination
Targets a protein for destruction
Protein A is a
trans-acting factor
In the absence of lactose, ___ binds to ____ and ___ lac operon transcription
The gene product of Lacl, LacO, represses
Which of the following conditions would lead to high expression of the lac operon
No glucose, Abundant lactose
If you want to study gene X mRNA, use
in situ hybridization
Transgenic C. elegans that express green fluorescent protein
Transgenic C. elegans expressing GFP contain DNA from a different organism
You are studying a mutant phenotype in Drosophila where flies have no wings. You sequence these flies and find a mutation in the wingless gene. The mutation, however, however, is not in the coding sequence of the gene but instead in the promoter sequence.
The mutation in promoter deleted a transcription factor response binding sequence leading to loss of wingless expression
Growth factor A has a response element
Gene A has a higher GF+ and small GF- and Gene B and C has same level of GF- and GF+
TFX
TFX binds to the promoter of gene X and recruits RNA polymerase to turn. upregulate gene X transcription
