Test 3 Flashcards
(56 cards)
What are the parts of a nucleotide?
sugar, phosphate, Nitrogen base (A,T,C,G)
Which pair of bases has 2 hydrogen bonds?
Adenine, Thymine
Which pair of bases has 3 hydrogen bonds?
Guanine, Cytosine
How are the strands of DNA made up?
They have: a chain of deoxyribose and phosphates, with bases attached to the deoxyribose
Between what two bases does bonding occur?
A hydrogen bond occurs between two bases
What is a complementary DNA strand?
The strand that directs the “complement” strand to synthesize a series of complement bases
What is the duplication of DNA?
- make a copy of the DNA
- chromosomes are now double-stranded
- This occurs in the S phase of Interphase
- Copy of DNA can now be passed on to new cells as single stranded chromosomes!
What is the process of DNA replication?
- H bonds between bases unzips
- This forms 2 single strands, and each strand serves as a template
- Forms 2 new complementary strands exactly like the beginning
How is DNA semi-conservative?
DNA has 1 parent strand and 1 daughter strand (one original and one new)
What is the chemistry and make up of RNA?
- sugar (ribose)
- Phosphate
- 4 bases (A,U,G,C)
RNA is single stranded!!
What are the three kinds of RNA?
mRNA, rRNA, tRNA
What is mRNA?
(messenger RNA)
long chain of bases which carries messages from DNA
They are read 3 base pairs at a time (codon)
What is rRNA?
(ribosomal RNA)
Combines with protein to form ribosomes
It makes two subunits (one larger and one smaller) to form structural protein
What is tRNA?
(transfer RNA)
Picks up an amino acid at one end
Has an anticodon at the other end
Then pairs with a codon in mRNA
How do RNA and DNA differ?
RNA uses ribose sugar, instead of deoxyribose, which has -H attached to the second ring
DNA is responsible for storing and transferring genetic information, while RNA directly codes for amino acids and acts as a messenger between DNA and ribosomes to make proteins
What are the steps for Transcription (converting DNA)?
- Starts with DNA in the nucleus because DNA is too big to leave
- DNA unzips a portion
- mRNA reads unzipped DNA
- Uracil in mRNA bonds with Thyamine
- mRNA unzips and moves into cytoplasm
mRNA takes DNA slightly changes it, and takes it into the cytoplasm
What is Translation?
Takes mRNA message and convert into proteins
- mRNA moves to cytoplasm
- attached to ribosome (rRNA) in cytoplasm (RNA is the structure where this occurs)
- mRNA codon determines which amino acid on the tRNA bonds
- tRNA anticodon binds to mRNA codon
- Amino acids from tRNA connect together by peptide bonds and MAKES PROTEIN
What are the subunits in a ribosome?
The subunits are Large and Small
In ribosomes, what does the Large subunit do?
The Large units contains the active site of the ribosome and creates the new peptide bonds when protein is synthesized
In ribosomes, what do Small subunits do?
The small subunit is in charge of information flow during protein synthesis. It reads mRNA and makes sure that each codon is properly paired up with its anticodon in tRNA
What is a plasmid?
A small extra piece of DNA that is used to carry DNA into other cells (vectors)
How are plasmids used in genetics?
They are where we insert changed DNA, which can change some of the make up of an organism
What is bacterial DNA?
Plasmids that have less DNA and fewer genes and lack a nucleus
What are restriction enzymes used for?
Used to cut and paste pieces of plasmids or bacterial DNA and look for a certain pattern in ATCG to find where to cut