TM

Question 1

Why do we add Coombs control cells to negative DAT/IAT?

Answer 1

To confirm AHG is added

AHG stands for Anti-Human Globulin, which is used in blood testing to detect antibodies.

Question 2

What can cause a false negative result under washed cells for IAT?

Answer 2

False negative result

IAT stands for Indirect Antiglobulin Test.

Question 3

What should be done with platelets received on ice?

Answer 3

Discard and record inventory

Question 4

If a patient has a history of antibodies but the antibody screen is negative, what antibody does she likely have?

Answer 4

Anti-Jka

Question 5

Fetal hemorrhage in Kleihauer Betke tests indicates what?

Answer 5

Maternal blood

Question 6

What is the optimum temperature for Rh antibodies?

Answer 6

37C

Question 7

Weak D test should be performed on which scenarios?

Answer 7

A & C

• Rh negative baby of Rh negative mom
• Rh negative donor

Question 8

What condition describes antigen excess in a precipitation reaction?

Answer 8

Postzone

Question 9

What are the possible genotypes for D+C+E+c+e+?

Answer 9

R1r’’ / R1R2

Question 10

What are the possible genotypes for D+C-E+c+e+?

Answer 10

R2r

Question 11

What is used to differentiate IgM and IgG?

Answer 11

Temperature

Question 12

What does Anti-M do?

Answer 12

Acidify the specimen

Question 13

If the mother is R2r and the father is rr, what antibody will the mother produce against the baby?

Answer 13

None

Question 14

When should antigen typing not be done?

Answer 14

If the patient has been transfused within 3 months

Question 15

If a patient has an antibody screen negative but a history of anti-K, what testing should be done?

Answer 15

Do IAT crossmatch

Question 16

What should be done if the BB automatic washer is not working?

Answer 16

Wash manually

Question 17

What should be done if Anti-Lea is detected at 28 weeks of gestation?

Answer 17

Report

Question 18

What type of cells should be issued next time for a patient who had fever, nausea, and low WBCs after transfusion?

Answer 18

Irradiated

Question 19

What can cause a false negative IAT due to heavy cell suspension?

Answer 19

Too many antigens due to having many RBCs

Question 20

What is it called when there is an antigen excess reaction?

Answer 20

Post zone

Question 21

Who requires RhIg?

Answer 21

Mom non-sensitized

Question 22

What is the storage temperature for bovine albumin?

Answer 22

4C

Question 23

What should be done if Anti-A antisera formed a reaction with A1 blood type but not with A2?

Answer 23

Remove reagent anti A from lot

Question 24

What should be done if Anti-A antisera did not react with A2 blood type?

Answer 24

Discard Antisera

Question 25

What should be done if Lea Ab is seen in a pregnant woman?

Answer 25

Report to physician

Question 26

What action should be taken if Anti-D is present with a titer of 32?

Answer 26

Call physician and send preliminary report

Question 27

What does the Jka antibody do?

Answer 27

Enhanced by enzyme. DHTR.

Question 28

Which one is NOT suitable for quality control?

Answer 28

More concentrated cell used

Question 29

What to do if Coomb’s control cells do not agglutinate in the final phase of the AHG test?

Answer 29

Repeat the test

Question 30

What to do if Coomb’s antisera test is negative?

Answer 30

Document and retest

Question 31

If a patient has anti-S but the antigen S is positive, what is the probable cause?

Answer 31

DAT

Question 32

What should be done if a sample is received in the transfusion lab with a spelling error?

Answer 32

Request new sample

Question 33

What can you NOT do on a patient with transfusion within the past 3 months?

Answer 33

Phenotyping

Question 34

What is the next step when an un-transfused open unit of packaged red blood cells is returned?

Answer 34

Discard the unit

Question 35

What should be done for an Rh negative mother pregnant with her second baby who has anti-D in her system?

Answer 35

No need for RhIg

Question 36

What term describes a blood sample that agglutinated in most all reagents?

Answer 36

Polyagglutination

Question 37

What tests are performed in cord blood?

Answer 37

ABO, Rh, DAT

Question 38

What is a likely reason for a negative DAT in a patient that had a transfusion reaction?

Answer 38

Donor’s RBC is cleared out in the system

Question 39

What should be done for a pregnant patient with anti-D and a titer of 32?

Answer 39

Call the doctor

Question 40

What to do if a computer crossmatch is not eligible for a patient that needs blood?

Answer 40

Perform serological crossmatch

Question 41

What may cause shock and diffuse hemorrhage after a blood transfusion?

Answer 41

Transfusion Reaction

Question 42

If no reaction occurred after adding Coomb’s Reagent, what should be done?

Answer 42

Repeat the test

Question 43

What antibody will not be detected after testing with papain?

Answer 43

Anti-S

Question 44

What blood type is needed for an exchange transfusion?

Answer 44

O negative

Question 45

What type of blood would probably be used in GVHR?

Answer 45

Irradiated blood

Question 46

If a patient has anti-K, anti-Fya, and anti-M, what should be done?

Answer 46

Transfuse K- Fya-

Question 47

What is the highest amount of IH?

Answer 47

Group O

Question 48

What will be the result if the IAT phase is prolonged to 60 minutes?

Answer 48

A & B * False positive * Denatured antibody

Question 49

What antibodies will a baby with DAT+ likely have?

Answer 49

Anti-D, Anti-Le

Question 50

What should be done if a latex agglutination test did not react with positive and negative controls?

Answer 50

Repeat positive control

Question 51

What will permanently defer a patient from being a donor?

Answer 51

On some medication and if Hep/HIV positive

Question 52

What will be the result if a unit of cryo is thawed and kept at room temperature for 2 hours?

Answer 52

A. Issue

Question 53

When is RhIG given?

Answer 53

26 weeks amniocentesis

Question 54

How frequently should you check the temperature of the blood storage fridge in BB?

Answer 54

A. Every 8 hours

Question 55

Why do we add Coombs control cells to all negative IAT/DAT tests?

Answer 55

To confirm that we added AHG to the test system