topic 2 Flashcards
What is the job of muscular tissue, glandular tissue and epithelial tissue?
Muscular-contracts to move whatever it’s attached to, and moves stomach wall to churn food. Glandular-makes and secrets chemicals e.g. enzymes and hormones, and makes digestive jucies to digest food. Epithelial-covers some parts of body e.g. inside of gut, and covers outside and inside of stomach.
What is a tissue?
group of similar cells that work together to carry out a particular function.
What is an organ?
Group of different tissues that work toghther to perform a certain function.
What is an organ system?
group of organs working together to perform a particular function.
What is an enzyme?
A biological catalyst. Enzymes reduce the need for high temperatures and we only have enzymes up to speed the useful chemical reactions in the body. A catalys is a substance which increases the speed of a reaction without being changed or used up in the reaction. Enzymes are all large proteins and all protiens are made up of chains of amino acids. These chains are folded into unique shapes, which enzymes need to do their jobs.
What is an active site and substrate?
Every enzyme has an active site with a unique shape that fits onto the substrate involved in a reaction. Enzymes useually only catalyse 1 specific reaction. Becuase for enzyme to work, substrate has to fit into its active site. If substrate doesn’t match enzyme’s active site, then the reaction won’t be catalysed.
What is the ‘lock and key model’?
See revison guide.
How can changing the temperature have an effect on the enzyme?
Higher temperature increases rate at first. But if gets too hot, some of bonds holding enzyme together break. Changes the shape of enzyme’s active site, so substrate won’t fit any more. Enzyme is said to be denatured. All enzyms have an optimum temperature that they work best at.
How can changing the pH have an effect on the enzyme?
If too high or low, pH interferes with bonds holding enzyme together. This changes shape of active site and denatures the enzyme. All enzymes have a optimun pH that they work best at. It’d often neutral pH 7. E.g. pepsin is an enzyme used to break down proteins in stomach. It works best at pH2, which means it is more suited to acidic conditions there.
What is amylase used for?
Breakdown starch to maltose. Easy to detect starch using iodine solution-if starch present iodine solution will change from browny-orange ot blue-black.
What are the first steps to test for starch?
Put a drop of iodine solution into every well of spotting tile. Place bundsen burner on a heat-proof mat, a tripod and gauze over bundsen burner. Put beaker of water ontop of tripod and heat water until its 35 Celcius (use thermometer to measure temperature). Try to keep temperature of water constant throughout experiment.
What do you do after you have measured the temperature of the water?
Use a syring to add 1cm3 of amylase solution and 1cm3 of a buffer solution with a pH of 5 to a boiling tube. Using test tube holdrs, put tube into beaker of water and wait for 5 minuites. Next use a differenet syringe to add 5cm3 of a starch solution to boiling tube. Immediatly mix contents of boiling tube abd start stop clock.
What do you do with continuous sampling?
Record how long takes foramylase to break down all of starch. To do this, use a dropping pipette to take a fresh sample from boiling tube every 30 seconds and put a drop into a well. When iodine solution remains browny-orange, starch is no longer present.
Why do we repeat the experiment with buffer solutions of different pH values?
To see how pH affects time taken for starch to be broken down. Remeber to control variables each time. (e.g. concentration and volume of amylase solution) to make it a fair test.
Does rate of reaction change over time?
Yes
What is the formula for rate of reaction?
rate=1000/time. Units aer in s-1 since rate is given per unit.
What does an experiment and calculations do?
Experiment: meaures how much something changes over time.
Calculation: can calculate rate of reaction by dividing amount that it has changed by time taken.
Why does digestion need to break dow food to smaller molecules?
so they can pass through walls of digestibe system easily, alowing them to be aborbed into blood stream.
What is the examle of carbohydrases convert carboyhdrates into simple sugars?
Amylase is example of carbohydrase-breaks down starch. see revision guide. Amylse found in slaivaryglands, pancreas and small intestine.
What is the example of proteases convert proteins into amino acids?
see revision guide
proteins are found in stomach (called papsin there)
pancreas
small intestine.
What is the example of lipase convert lipids into glycerol and fatty acids?
See revision guide. Lipases found in pancreas and small intestine.
What are the products of digestin used as?
new carbohydrates, proteins and lipids. Some of glucose (carbohydrate) that is used in respiration.
What is the example of bile to emulsifie fat?
Produced in liver, stored in gall bladder before released into small intenstine. Hydrochloric acid in stomach makes ph too acidic for enzymes in small intestine to work properly. Bile is alkalint-neutralises acid and makes conditions alkaline. Enzyme in small intestine works best in these alkaline conditions. It emulsifies fats. It breaks down fat into tiny droplets. Gives much bigger surface area of fat for enzyme lipase to work on-makes digestion faster.
What happens in the salivary glands?
produce amylase enzyme in saliva.