Topic 2 Investigating Enzamatic Reactions #

Question 1

How pH affects amylase activity steps 1-3

Answer 1

1) put a drop of iodine solution into every well of a spotting tile
2)place a Bunsen burner on a heat proof mat and a tripod and gauze over the Bunsen burner. Put a beaker of water on the top of the tripod and heat the water until it is 35°c (use thermometer to measure temp) try to keep temp constant throughout the experiment
3) use a syringe to add 1cm3 of amylase solution and 1cm3 of buffer solution with a ph of 5 to a boiling tube. Using test tube holders put the tube into the beaker of water and wait 5mins

Question 2

How pH effects amylase activity steps 4-6

Answer 2

4)Next use different syringe to add 5cm3 of starch solution to the boiling tube
5) immediately mix the contents of the boiling tube and state the stop clock
6)use continuous sampling to record how long it takes for the amylase to break down all of the starch. To do this Use dropping pipette to take a fresh sample from the boiling tube every 30 seconds and put a drop into a well. When solution remains browny orange starch is no longer present

Question 3

How do you know when no starch remains

Answer 3

When the solution remains browny orange

Question 4

How pH affects amylase activity 7

Answer 4

Repeat the wile experiment with buffer solutions of different pH

Question 5

What is the control variable for how ph affects amylase activity

Answer 5

Concentration and volume of amylase solution

Question 6

What is rate of reaction used for

Answer 6

Rate is a measure of how much something changes over time

Question 7

Rate if reaction calculation

Answer 7

Rate = 1000 / time