Topic 4 Flashcards
(46 cards)
Give 3 differences between prokaryotic DNA and eukaryotic DNA
prokaryotic DNA
Shorter
Circular
Not associated with proteins
Eukaryotic DNA
Longer
Linear
Associated with histones
Which 2 organelles contain prokaryotic DNA
Mitochondria and chloroplasts
What is a gene
A base sequence of DNA that codes for amino acid sequence of a polypeptide or functional RNA
What is a locus
The fixed position of a gene on a chromosome
What are 3 features of genetic code
Degenerate - multiple codons can code for the same amino acid during photosynthesis
Universal - the same triplet of based codes for the same amino acid in all organisms
Non- overlapping - each base in a gene is only part of one triplet therefore each codon is read as discrete unit
What are introns
Sections of DNA that do not code for polypeptides
Where are introns found
In eukaryotic DNA not in prokaryotic
What are exons
Sequenced of DNA that DO code FOR AMINO ACIDS
What is a codon
Three bases on mRNA that code for a specific amino acid
What is a start codon
Three babes that start of every green that initiates translation
What is a stop codon
3 bases at the end of every gene that causes ribosomes to detach and therefore stop translation
What is a genome
An organisms complete set of genes in a cell
What is a proteome
The full range of proteins that a cell is able to produce
Give the properties of mRNA
(4)
short single stranded
Found in the cytoplasm and nucleus
Made during transcription complementary to DNA sequence
Give some properties of tRNA
Is clover shaped
Single stranded
Each tRNA molecule has three based called an anticodon these are complementary to codons on the mRNA molecule
tRNA is involved in translation as it carries amino acids used to make proteins to the ribosomes
What is transcription
Where one gene on the DNA is copied into mRNA
What is translation
Where the mRNA joined with a ribosome and corresponding tRNA brings the specific amino acid the codon codes for
Why is mRNA used for transcription
MRNA is much shorter than DNA And so is able to carry genetic code to ribosome in the cytoplasm
Give the 5 steps for transcription
- DNA helix unwinds to expose bases to act as template
- Only one chain of DNA acts as template
- This is catalysed by DNA helicase which breaks down the hydrogen bonds between bases
- Free mRNA nucleotides align with exposed complementary bases
- Enzyme RNA polymerase joins them together this RNA is then modified and leaves nucleus though nuclear envelope pores
In eukaryotes what is made after transcription and how
Pre mRNA made from mRNA that still contains introns so introns need it be spliced out by a protein caked a spliceosome
In 6 steps explain the process of translation
- Once modified mRNA left nucleus attached to a ribosome in the cytoplasm
- Ribosome attached at the start codon
- tRNA with the complementary anti codon to start codon aligns
- Ribosome will move along one corn on the mRNA molecule to enable another complementary tRNA to attach to the next codon on mRNA
- 2 amino acids that have been delivered by the tRNA molecule joined with a peptide bond catalysed by an enzyme and requires ATP
- This continues until ribosome reaches stop codon this stop codon does not code for an amino acid and so ribosome detached and translation ends
What is a mutation?
A change in the base sequence of DNA
What are mutagenic agents give 2 examples
Interfere with DNA replication icy as UV light, ionising radiation and chemicals and carcinogens such as cigarette smoke
What are the 2 forms of gene mutation
Deletion or substitution