Topic 5 Flashcards

(26 cards)

1
Q

what is energy used for

A

movement, metabolic reactions, active transport, cell division, protein synthesis

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2
Q

cristae

A

folds of inner membrane of mitochondria which increases the surface area

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3
Q

where does glycolysis occur

A

cytoplasm

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4
Q

glycolysis

Overall
Steps (4)
Net ATP for one glucose molecule

A

Splitting of one 6C glucose molecule into two 3C pyruvate molecules

Hydrolysis of ATP produces phosphate molecules
Glucose is phosphorylated and becomes more reactive
- (Ea is lowered) -2ATP
Phosphorylated glucose splits into two 3C glycerate 3-phosphate (GP) molecules
Two GP molecules convert into two 3C pyruvate molecules
- (+2ATP and NAD -> NADH + 3ATP) x2
Net ATP for one glucose molecule: +8

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5
Q

where does the link reaction occur

A

mitochondrial matrix

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6
Q

link reaction

Steps (3)
Net ATP for one glucose molecule

A

Two pyruvate molecules are actively transported into the matrix of mitochondria
Two pyruvates combine with two co-enzyme A to form two 2C Acetyl coA molecules
Each releases one CO2 and NAD -> NADH + 3ATP

Net ATP for one glucose molecule: +6

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7
Q

where does the Krebs cycle occur

A

mitochondrial matrix

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8
Q

Krebs cycle

Steps (3)
Net ATP for one glucose molecule

A
For each molecule of 2C Acetyl coA joins with 4C oxaloacetate to form 6C citrate
6C citrate -> 5C + CO2
    - NAD -> NADH + 3ATP
    - ADP + Pi -> ATP
5C -> 4C oxaloacetate + CO2
    - 2NAD -> 2NADH + 6ATP
    - FAD -> FADH + 2ATP
  • Net ATP for one glucose molecule: +24
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9
Q

Total net ATP for one glucose molecule in aerobic respiration

A

8+6+24= 38

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10
Q

where does oxidative phosphorylation occur

A

inner mitochondrial membrane/ on cristae

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11
Q

oxidative phosphorylation

Generally wth is it
Steps (6)

A

Transfer of electrons along a series of electron carriers generates ATP in electron transport chain

Reduced NAD (NADH) is oxidised to NAD and releases hydrogen ions to the electron transport chain and returns to the Krebs cycle
Electrons are carried along a chain of electron carriers in a series of redox reactions
Released energy is used to pump protons from the matrix to intermembrane space by active transport, creating a diffusion gradient
Chemiosmosis: protons diffuse back into matrix via pores with protein channels and ATP synthase
Energy is released which is used for ADP + Pi -> ATP
Oxygen is the terminal electron acceptor which forms water

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12
Q

why is anaerobic respiration important

A

regenerates NAD for more glycolysis to produce ATP

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13
Q

anaerobic respiration in animals

A

each pyruvate accepts 2H from 2NADH to form lactic + 2NAD

reversible reaction, transported via blood to be conveted to glucose in the liver

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14
Q

anaerobic respiration in plants

A

each pyruvate loses 1CO2 and accepts H from NADH to form ethanol + CO2 + NAD
irreversible reaction

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15
Q

Absorption spectra

A

the range of different wavelengths of light a photosynthetic pigment can absorb

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16
Q

Action spectra

A

compares rate of photosynthesis with the wavelength of light

17
Q

where does the light dependent reaction occur

A

thylakoid membrane of the chloroplasts

18
Q

cyclic phosphorylation

A

Light hits chlorophyll in PS1 and excites an electron to leave chlorophyll molecule

Electron is picked up by an electron acceptor and passed along electron carriers to produce ATP

Electron returns to chlorophyll molecule

19
Q

non-cyclic phosphorylation

A

Water molecule splits by photolysis releasing electrons to chlorophyll

Light hits chlorophyll in PS2 and excites an electron to leave chlorophyll molecule

Electron is picked up by an electron acceptor and passed along electron carriers to produce ATP

PS1 receives the electron which is excited by light again

Electron is picked up by an electron acceptor and passed along electron carrier to be picked up by another electron acceptor (NADP)

NADP takes up H+ from water molecule to form NADPH

20
Q

photolysis of water

A

in chloroplast
H2O -> H+ + OH-
H+ used to reduce NADP

4OH- -> O2 + 2H2O + 4e
oxygen as a waste product
electrons restore chlorophyll to original state

21
Q

where does the light independent reaction occur

A

stroma in chloroplasts

22
Q

light independent reaction

A

CO2 diffuses into the plant via stomata and fixes with 5C ribulose bisphosphate (RuBP) using ribulose bisphosphate carboxylase (RUBISCO) enzyme to form a 6C compound

6C compound splits into two 3C GP

GP is reduced to form 3C glyceraldehyde 3-phosphate (GALP)

H involved is from NADPH and energy required comes from ATP which were produced in the LDR

Some GALP synthesises a 6C β-glucose molecule by condensation

23
Q

what happens to glucose synthesised in photosynthesis (4)

A

May pass into the glycolysis pathway to provide energy in the form ATP and acetyl coA which is used to synthesise fatty acids

May be converted to sucrose for transport or starch for storage

Straight polymer cellulose formed by glycosidic bonds between C1 and C4

24
Q

what happens to GALP synthesised in photosynthesis (3)

A

Some GALP synthesises a 6C β-glucose molecule by condensation

Some GALP combines compounds from respiration with nitrates from the soil to form parts of amino acids

Some GALP regenerates into 5 C RuBP for the first step of the cycle

25
DCPIP colour change
DCPIP dye turns blue to colourless when reduced LDR would reduce NADP to NADPH but reduces DCPIP instead absorbance of light decreases
26
role of the Krebs cycle
``` completely oxidises pyruvate/ acetyl coA to release as much energy as possible to directly generate ATP to produce NADH so ATP can be produced by oxidative phosphorylation ```