TRANS (Part 2) Flashcards
(106 cards)
1
Q
Microscopically with tapered ends appearing like toothpick
A
Fusobacterium
nucleatum
2
Q
With “bifid” ends/appearing like bone shaped dog biscuit
A
Bifidobacterium
3
Q
- Anaerobic bacilli that assume safety pin appearance and may show resistance to Kanamycin, Vancomycin and Colistin in Potency disk test
- Bile tolerant
- Can cause aspiration pneumonia
A
Bacteroides fragilis
4
Q
Anaerobic diphtheroid
A
Propionibacterium spp.
5
Q
Spot Indole Test (+) organism; color
A
Cutibacterium acnes
(+) Blue/Green
6
Q
Spot Indoles Test (-) organism; color
A
Propionibacterium spp.
(-) Pink/orange
7
Q
- Reagent used in spot indole test
- Same reagent used in PYR test for S. pyogenes (major throat pathogen
A
P-dimethylaminoacinamaldehyde
8
Q
Used to verify gram staining result of anaerobic bacteria
A
Potency Disk test
9
Q
What are the antibiotics used in Potency disk test, give their amount
A
- Kanamycin (1000 ug)
- Vancomycin (5 ug)
- Colistin (10 ug)
10
Q
KVC
RRR
A
B. fragilis
11
Q
KVC
SRS
A
Fusobacterium, B. ureolyticus, Veilonella
12
Q
KVC
SSR
A
Clostridium
13
Q
KVC
RSR
A
Porphyromonas, P. anaerobius
14
Q
KVC
RRS
A
Prevotella
15
Q
SPS (Sodium Polyanethol Sulfonate) - sensitive
A
P. anaerobius
16
Q
Remedy to counteract the inihibitory property of P. anaerobius
A
Add 1% gelatin
17
Q
Anticoagulant often use in bacterial culture
A
0.25% SPS
18
Q
Shows swarming on BAP
A
C. tetani and C. septicum (Spore forming anaerobes)
19
Q
- Can develop colonies appearing like molar tooth
- Can cause actinomycosis of Jaw – Lumpy jaw (affecting soft tissue)
A
Actinomyces Israelii
20
Q
Anaerobic staphylococcus
A
Peptococcus niger
21
Q
Anaerobic streptococcus
A
Peptostreptococcus
22
Q
Important component of dental biofilm
A
Prevotella
23
Q
A versatile media an often used in the lab because it can allow growth of anaerobe, facultative anaerobes and aerobes
A
Thioglycolate
24
Q
Growth at the bottom
A
Anaerobes
25
Diffuse growth all throughout the media
Facultative anaerobes
26
Growth towards the surface
Strict Aerobes
27
- Gram negative organism
- Strict aerobes
- Significant organisms: B. pertussis, B. bronchiseptica, B. parapertussis they can cause respiratory disease
Genus Bordetella
28
Recommended media for Bordetella
Regan Lowe
29
Traditional media used to isolate Bordetella
Potato Blood Glycerol agar
30
Motile Bordetella
B. bronchiseptica
31
Non-motile Bordetella
B. pertussis and B. parapertussis
32
Refers to F. tularensis (accd. to Mahon)
Jellison Type A
33
- A potential bioterrorism agent
- Unable to grow on ordinary media, requires special media
- Pathogenic and may cause Tularemia, Market men rabbit fever, deerfly fever, Lemming disease, water rat trapper disease
F. tularensis
34
With special requirement for cystine, cysteine and thiosulfate
F. tularensis
35
Used to cultivate F. tularensis
Blood Cystine Glucose Agar (BCGA)
36
- Genus considered as animal flora, usually found in placental tissues (they cause abortion in animals)
- Strict aerobes
- Inhibited by dyes
- Also an agent of Bioterrorism
Genus Brucella
37
Bang’s bacillus
Cattles
B. abortus
38
Goat
B. melitensis
39
Swine/Pigs
B. suis
40
B. canis
Dogs
41
Brucella species not classified as bioterrorism agent and not detected thru SAT
B. canis
42
Rapid test for Brucellosis detection
Serum Agglutination Test (SAT)
43
Significant titer of SAT
1:160
44
Other terms for Brucellosis
Mediterranean fever / Malta fever / Undulant fever / Gibraltar fever in man (a febrile disease)
45
Optimal specimen for brucellosis detection
Blood
46
Routine blood culture may be done for
7 days
47
To detect brucellosis, it may take
3-4 weeks
48
Inhibited by thionine (will not grow on media with thionine)
B. abortus
49
Inhibited by basic fuchsin (will not grow on media with basic fuchsin)
B. suis and B. canis
50
L. pneumophila causes
Legionnaire’s disease (form of pneumonia) and Pontiac fever (nonpneumonic forn, febrile dse)
51
Isolation media for L. pneumophila because it cannot grow on ordinary media
Buffered Charcoal Yeast Extract (BCYE)
52
HACEK members are causative agent of
Subacute Bacterial Endocarditis (SBE)
53
- Agent of Human bite wound/Clenched fist wounds
- Part of HACEK, normal flora but can cause SBE
- Pits and corrodes agar
- Colonies with bleach like odor
- Assacharolytic
Eikenella corrodens
54
Animal bite wound, colonies with musty odor
Pasteurella multocida
55
Normal flora of oral cavity but can cause SBE
H. parainfluenzae
56
- Previously under Haemophilus
- Normal flora but can cause SBE
Aggregatibacter aphrophilus
57
- Star shaped colonies
- Grows together with A. israelii
Aggregatibacter actinomycetemcomitans
58
- Twitching motility
- Can cause infection of the bones and heart SBE
Kingella Kingae
59
Rosette arrangement on gram-stained smears
Cardiobacterium hominis
60
Regarded as nonfermenters (not normal flora and usually found in hospital), oxidizers which is capable of producing acids only under aerobic condition/presence of air
- Genus Pseudomonas
- Genus Stenotrophomonas
- Genus Burkholderia
- Genus Acinetobacter
61
- #1 ICU isolate,
- Strict aerobe, oxidase (+), beta hemolytic on BAP
- Produces colonies with and odor similar to an overripe grapes, taco-corn tortilla-like odor
- Can develop both pyocyanin and pyoverdin
P. aeruginosa
62
Agent of pneumonia in those with cystic fibrosis, causes ecthyma gangrenosum, jacuzzi or hot tub syndrome because it can grow on wet environment
P. aeruginosa
63
Media for initial isolation of P. aeruginosa
Toxic to many bacteria
Cetrimide Agar
64
Non-motile and oxidase (-)
Another nonfermenter
Genus Acinetobacter
65
Nonfermenter
Significant pathogens: B. mallei and B. pseudomallei
Genus Burkholderia
66
- Also known as Glander’s bacillus
- Glander’s disease (respiratory dse.)
B. mallei
67
- Also known as Whitmore’s bacillus
- Melioidosis (respiratory dse.), Vietnam time bomb
B. pseudomallei
68
Colonies with earthy odor on ashdown media
B. pseudomallei
69
Both capable of producing wrinkled colonies
P. stutzeri and P. pseudomallei
70
Both capable of producing wrinkled colonies
P. stutzeri and P. pseudomallei
71
Cause transfusion associated septicemia
P. fluorescens and P. putida
72
Compared to P. aeruginosa, P. fluorescens and P. putida can only develop
Pyoverdin
73
Differential test for P. fluorescens and P. putida
Gelatin Hydrolysis test
74
P. fluorescens: Gelatin Hydrolysis result
Positive
75
P. putida: Gelantin Hydrolysis result
Negative
76
Detect fermentation or acid production
OF (Oxidative Fermentative) media of Hugh and Leifson
77
- High carbohydrate (CHO) but low peptone content
- CHO = Glucose
OF (Oxidative Fermentative) media of Hugh and Leifson
78
pH indicator of OF (Oxidative Fermentative) media of Hugh and Leifson
Bromthymol blue
79
Open tube is for
Aerobic culture
80
Closed tube is for
Anaerobic culture
81
Color of positive result in OF Media (H&L)
Yellow
82
Color of negative result in OF Media (H&L)
Green/Blue green
83
Interpret: (+) in both open and closed tubes
FERMENTER (organism was able to produce acid with or without air)
84
Interpret: (+) in open and (-) in closed tube
OXIDIZER/NON-FERMENTER (organism was able to produce acid only under aerobic condition but not anaerobically, oxidizer are seldom used term in the lab)
85
Interpret: (-) in both open and closed tube
NON-OXIDIZER/NON-SACCHAROLYTIC (organism was unable to produce acid aerobically and anaerobically)
86
Indicator in OF media – CDC Method
Phenol red
87
Color of positive result (CDC method)
Yellow
88
Color of negative result (CDC method)
Red
89
Usual media to detect acid production of Enterobacteriaceae
TSI (Triple Sugar Iron)
90
pH indicator of TSI
Phenol red
91
TSI contains 3 fermentable carbohydrated
- 1 part glucose
- 10 parts sucrose
- 10 parts lactose
92
TSI media can also be used as H2S indicators because it has
ferrous sulfate and sodium thiosulfate
93
Acid (+): Color
Yellow (A)
94
Alkaline (-): Color
Red (K)
95
Interpret: Red slant and butt
K/K = Non-fermenter
96
Intrepret: Red slant and Yellow butt
K/A = Non-Lactose fermenter
97
Example of Non-Lactose fermenter
(Shigella spp. PPM)
98
Interpret: Yellow slant and Yellow butt
A/A = Lactose fermenter
99
Example of Lactose fermenter
E.coli, Enterobacter, Klebsiella
100
Example of Late Lactose fermenter
Citrobacter, Y. enterocolitica, S. sonnei
101
Usual media to detect acid production of non-fermenters increased CHO and Low peptone content
OF Media of Hugh and Leifson
102
TSI result of non-fermenters
K/K (P. aeruginosa, strict aerobe)
103
TSI result of Enterobacteriaceae (All glucose +, facultative aerobe)
K/A or A/A
104
OF media result of Enterobacteriaceae
Both (+) in open and closed tube
105
OF media result of non-fermenters (P. aeruginosa)
(+) open tube and (-) for closed tube
106
TSI result of non-lactose fermenters
K/A
