Translation Flashcards

(41 cards)

1
Q

in translation, a sequence of ___ is translated into a sequence of __

A

nucleotides; amino acids

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2
Q

the genetic code gives the relation between __ and __

A

sequence of bases in DNA and of amino acids in proteins

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3
Q

T/F the genetic code is nearly universal

A

true

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4
Q

the genetic code is read from the _ to __ end of the mRNA

A

5’-3’

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5
Q

an amino acid is coded by a group of 3 bases called a __

A

codon

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6
Q

T/F there is only one codon for each amino acid

A

false

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7
Q

what is protein synthesis?

A

formation of peptide bonds between amino acids in a specific sequence

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8
Q

what are ribosomes made of?

A

rRNA and protein

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9
Q

a ribosome can be called a ___

A

ribonucleoprotein

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10
Q

what part of a ribosome catalyzes the peptide bond formation?

A

rRNA

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11
Q

function of small ribosomal subunit

A

binds mRNA

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12
Q

function of large ribosomal subunit

A

binds tRNA and amino acids

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13
Q

ribosomes are typically identified by their Svedberg coefficient (s) which is __

A

rate at which particle moves during centrifugation

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14
Q

what three factors influence the Svedber coefficient?

A

particle mass, density and shape

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15
Q

rRNA molecules are ___ stranded

A

single

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16
Q

how do rRNA molecules achieve their complex secondary /teriary structures to be able to function?

A

base pair

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17
Q

a ribosome has 3 slots for tRNA which are ___

A

A site, P site, E site

18
Q

amino acids are delivered to the ribosome by__molecules

19
Q

tRNA serves as the adapter molecule between the ___ and its specific ___

A

codon; amino acid

20
Q

tRNAs are usually between ___ and ___ nucleotides long

21
Q

all tRNA molecules include what two things?

A

a CCA amino acid attachment site at the 3’ end

an anticodon

22
Q

what is an anticodon?

A

3 nucleotides that are complementary to the codon on mRNA

23
Q

the proper amino acid is also called the __

A

cognate amino acid

24
Q

what dictates the proper amino acid?

A

anticodon loop base pairing with the codon

25
tRNAs are covalently labeled with their cognate amino acids by enzymes called___
aminoacyl-tRNA-synthetases
26
charged tRNA is called
aminoacyl tRNA
27
what are the 2 steps in aminoacyl tRNA synthetase activity?
1. activation of the amino acid | 2. transfer of the aminoacyl group to the tRNA
28
how is an amino acid activated?
adenylation
29
what is used to adenylate the amino acid?
ATP
30
how is the amino acid transferred to the tRNA?
ester linkage
31
t/f aminoacyl tRNA syntheses are highly specific for a given amino acid
treue
32
what are the 3 steps of translation?
1. initiation 2. elongation of polypeptide 3. termination
33
what 3 things happen in the initiation of translation?
assembly of ribosome, binding of mRNA and binding of first Aa-tRNA
34
what 2 things happen in the enlogation of polypeptide of translation?
translocation and peptide bond formation
35
what happens in the termination of translation?
recognition of the end of the coding region in mRNA
36
what is the start codon and what amino acid does it code for?
AUG; methionine
37
catalysis of peptide bond formation occurs in the ___ site of ribosomes
P (peptide)
38
peptide bond formation is catalyzed by which ribosomal subunit?
the large one
39
what are the 3 stop codons?
UAA, UGA, UAG
40
T/F there is a tRNA with an anticodon for the stop codons
false
41
what is the role of a release factor protein?
will bind to the A site and release the polypeptide chain