Tutorial 2 safety equipment Flashcards
(9 cards)
What are the classes of Biological safety cabinets (BSCs) distinguished by?
- The level of personnel and environmental protection provided
- The level of product protection provided
When do you use a BSC?
- Handling infectious materials
- Increased risk of airborne infection
BSCs has a air filtration system called? State its function and traits.
High Efficiency Particulate Air (HEPA) Filters.
* 99.97% to 99.99% efficient at the most penetrating particle size ~0.3 um
* Remove particles from the air stream
-Interception
-Inertial impaction
-Diffusion
-Electrostatic charge
Describe Class I biological safety Cabinets (BSCs) based on how it operates and the protection it offers.
-Room air is drawn in through the
front opening, passes over the work
surface and is discharged through the
exhaust duct
-Directional airflow removes any
aerosol particles generated on the
work surface away from the operator
and into the exhaust duct
Protection:
-personnel protection
-environmental protection
-Does not protect product from contaminated room air.
Describe Class II biological safety Cabinets (BSCs) based on how it operates and the protection it offers.
-Allows only HEPA-filtered air supply to
flow over the work surface
-Four types (AI, AII, BI and BII) based
on construction type, airflow velocities
and patterns, and exhaust systems
-used for risk group 2 and 3. (4 with positive pressure suit)
Protection:
-personnel protection
-environmental protection
* Protects product (work materials)
from contaminated room air.
Describe Class III biological safety Cabinets (BSCs) based on how it operates and the protection it offers.
-Sealed to provide maximum
containment (for high risk agents) or positive pressure suit (used in BSL-4 cabinet
laboratories.
-work with attached rubber sleeves
- Supply air is HEPA filtered; Exhaust
air is double HEPA filtered
- Decontamination required prior to
removing work materials
Protection:
-personnel protection
-environmental protection
* Protects product (work materials)
from contaminated room air.
What are Laminar flow clean benches? And its protection?
workbench with own filtered air supply, used to work with non hazardous materials where clean, particle-free air quality is required.
Protection:
- Provides product protection only
* DOES NOT provide protection to
personnel or to the environment
* Not designed to contain aerosols
What are chemical fume hoods? Protection?
-Ventilation device that limits exposure to
hazardous or toxic fumes, vapors or dusts,
- independent exhaust system and operates with single-pass air discharged outside the building.
-Used when working with volatile chemicals
or chemical carcinogens
-Do not have HEPA filters
- DO NOT USE FOR BIOHAZARDOUS MATERIAL.
Protection:
-Only protects the worker from exposure
to chemicals and noxious gases
What are centrifuge?
device that spins liquid samples at
high speed around a central axis
