Twist Probe study guide Flashcards
(8 cards)
Key advantage of the twist probes
- High accuracy and precision.
(accurate probes ensure reliable, reproducible results which is essential for applications like NGS)
- Because of our silicon based platform, it allows for the production of probes with extremely low error rates.
2nd- scalability and customization
The probes can be tailored for specific sequences and scaled to meet the demands of any project, large or small.
- High throughput
Twist can produce millions of unique probes simultaneously.
- Binding and Hybridization capabilities
The probes are engineered in a way that they can bind (hybridize) strongly to their target DNA or RNA sequences. This is important because efficient hybridization, ensures that probes detect targets with high specificity, eliminating any “background noise”.
- Very important** Explain Biotinylated probes
This is where we shine. We add a biotin group to the probe structure which allows for easier detection and capture using streptavidin surfaces. This is important because you need to isolate or enrich specific target sequences. This allows for easier identification and analysis of the target DNA/RNA.
- Ability to capture sense and anti-sense strands
Twist probes can capture both the sense (coding strand) and antisense (complementary strand) sequences in DNA/RNA. By capturing both strands, you ensure that you accurately assess the full range of transcript variants. Some could be missed if there is a “bias” capture mentality.
Important when looking for insights on gene expression.
- Fast turnaround time
Rapid production of probes without sacrificing quality, speeding up research and diagnostic workflow.
Summary of the twist probes
- High accuracy and precision
- Scalability and customization
- High throughput
- Biotinylated probes
- Capture of both sense and antisense strands.
- cost effective
- Fast TAT
- QC checks
