Unit 1 Flashcards

(42 cards)

1
Q

Types of medical interventions

A

Genetics: braille, gene therapy, bone marrow transplant
Medical aids: glasses, braces, insoles, contacts
Medicine: advil, Tylenol, ibuprofen
Surgery: Botox, transplant, nose job
Rehab: crypto therapy, acupuncture, chemotherapy
Diagnostics: MRI scans, blood tests, thermometer
Immunology: ELISA, vaccines, antibiotics
Hygiene: comb, shampoo, floss

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2
Q

What is an outbreak

A

Is a sudden rise in the number of cases of a disease

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3
Q

Steps of ELISA

A

1: antigens in the sample bind to the sides of the wells
2: primary antibody binds to the specific antigen
3: secondary antigen (conjugated to an enzyme) binds to primary antibody
4: substrate reacts with the enzyme to produce a color change

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4
Q

Examples of ELISA tests

A

Pregnancy and COVID tests

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5
Q

Antibody/antigen

A

Antigens cause disease while antibodies fight them

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6
Q

Pathogen

A

A tiny thing that causes disease

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7
Q

Antigen-antibody complex

A

Complex formed by the binding of an antibody to an antigen and initiate immune responses

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8
Q

steps of BLAST

A

• Step 1: Given query sequence Q, compile the list of possible words which form with words in Q high scoring word pairs.
• Step 2: Scan database for exact matching with the list of words complied in step 1.
• Step 3: Extending hits from step 2.
• Step 4: Evaluating significance of extended hits from step 3.

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9
Q

What is BLAST used for

A

to identify portions that align with or are the same as existing sequences.

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10
Q

Explain how antibiotics prevent growth of E. coli on specific bacterial plates- how & why?

A

disrupt essential processes or structures in the bacterial cell. This either kills the bacterium or slows down bacterial growth. With a specific antibiotic, it would be able to prevent the growth of E. coli.

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11
Q

Why did the E. coli I strain grow on both the LB agar plate and the LB agar plate with streptomycin but not grow on the LB agar plate with ampicillin?

A

It did not grow on the LB agar plate with ampicillin because it was not affected by the specific antibiotic to stop the growth of the bacteria. The ampicillin plate was affected by the antibiotic resulting in no bacteria growth

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12
Q

Transformation

A

a bacterium takes up a piece of DNA floating in its environment

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13
Q

Transduction

A

DNA is accidentally moved from one bacterium to another by a virus

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14
Q

Conjugation

A

DNA is transferred between bacteria through a tube between cells.

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15
Q

difference between gram positive and gram negative bacteria

A

(+)
1 thick
no outer lipid membrane
purple
exotoxins
stains easier
few patho
inner membrane
treatable antibiotics
(-)
2 thin
outer lipid membrane
pink
endotoxins
harder to stain
tend to be patho
inner/outer membrane
harder to treat antibiotics

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16
Q

How do antibiotics work?

A

A substance produced by or derived from a microorganism and able in dilute solution to inhibit or kill another microorganism

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17
Q

What happens if you finish your medication and what happens if you don’t?

A

Even if your symptoms go away, the bacteria may still be present in your body. If you stop treatment before the antibiotic cycle is over, the remaining bacteria can continue to multiply and become resistant to antibiotics.
They may directly attack the bacterial cell wall, which injures the cell.

18
Q

differences between frequency & amplitude.

A

Frequency is a measurement of cycles per second, and amplitude is a measurement of how large a wave is

19
Q

difference between conductive & sensorineural hearing loss, where they occur, examples, and interventions for both.

A

Conductive hearing loss - occurs when sound conduction is impeded through the external ear, the middle ear, or both.
Intervention: hearing aids
Sensorineural hearing loss - occurs when there is a problem within the cochlea or the neural pathway to the auditory cortex.
Intervention: Hearing aids, cochlear/brainstem implants

20
Q

the outer part of the ear that takes the sound waves and puts them into the ear canal that will amplify the sounds.

21
Q

where sound waves enter

A

Auditory canal

22
Q

drains fluids and balances air pressure within the ear.

A

Eustachian tube

23
Q

allows the transmission of sound waves by attaching the tympanic membrane to the inner ear

A

Ossicles (malleus, incus, and stapes)

24
Q

acquires the vibrations from the outer ear and takes them to the ossicles in the middle ear and acts as a protection barrier.

A

Tympanic membrane (eardrum)

25
interprets the sound vibrations into electrical impulses so the brain can determine each sound frequencies.
Cochlea
26
supports and balances the transmission of sound vibrations into electrical impulses that get relayed to the brain.
Sensory hair cells
27
transports the generated electrical signals from the cochlea to the brain.
Cochlear nerve
28
initiates hearing receptors and the fluid fluctuations in the cochlea.
Oval window
29
transmits signals to the brain with different strengths in velocity.
Vestibule
30
balances the cochlear nerve.
Vestibular nerve
31
How vaccinations work
Vaccines work by having a weak antigen that will alert the immune system to respond within the body. This produces a “memory” in the body. This will help prepare the body to fight off the infection when recognized.
32
Harmless; contain recognizable antigens on the surface Microbes don’t multiply, needing boosters or more doses
Killed whole cells or inactivated viruses
33
Vaccines last longer & require fewer boosters Disease agent can mutate back into pathogenic strain
attenuated (weakened) cell or viruses
34
Purified toxin produced by the antigen used to elicit immune response
Toxoid Vaccines
35
Genes for microbial agents are inserted into a plasmid vector and are clones in appropriate hosts The resultant protein product is used to provoke immune response
Genetically engineered microbes/ microbial agents
36
These vaccines contain all or part of the pathogen DNA, which is used to “infect” a recipient’s cell
DNA Vaccines
37
Types of administered vaccines
Subcutaneous Intramuscular Intradermal
38
role of an epidemiologist in an outbreak
search for the cause of disease, identify people who are at risk, determine how to control or stop the spread or prevent it from happening again
39
What is herd immunity and how does it work?
Herd immunity occurs when a large portion of a community (the herd) becomes immune to a disease More immune people = decreases incidence of the disease and occurrence of the pathogen
40
attack rate
Attack rate = number of people at risk who develop a certain illness/the total number of people at risk
41
sources of an outbreak
Bacteria, viruses, parasites
42
steps of bacterial transformation
Step [1] Remove Plasmid from bacteria cell. Step [2] Isolate the gene of interest. Step [3] cut open plasmid with restriction enzymes, leaves "Sticky ends". Step [4] insert gene of interest. Step [5] Insert the Plasmid with Recombinant DNA into a new bacterium. (1) competent cell preparation - (2) transformation of cells (3) cell recovery (4) cell plating