Unit 1

Question 1

What is an assay

Answer 1

process of determining the amount of an analyte in a sample

Question 2

Signal to Noise Ratio Eq

Answer 2

S/N= k sqrt(number of measurements)

Question 3

What is a sample matrix/blank

Answer 3

Sample with everything but analyte

Question 4

Limit of Detection

Answer 4

Signal= Blank +3(standard deviation of blank)

Question 5

Limit of Detection

Answer 5

Signal= Blank +10(standard deviation of blank)

Question 6

Positive control

Answer 6

Prevents false negative results/ will always show the signal

Question 7

Negative Control

Answer 7

Prevents false positive results/ will never show the signal

Question 8

Selectivity

Answer 8

How much does interferences impact it

Question 9

Systematic vs Random error

Answer 9

Systematic is reproducible random is not

Question 10

Standard Addition

Answer 10

Use slope of line to extrapolate the x-intercept

Question 11

Standard Addition benefit

Answer 11

Accounts for matrix effects

Question 12

Internal Standards

Answer 12

Use a compound that behaves similar to the analyte

Question 13

Internal Standard Equation

Answer 13

Sa/Ca= k Si/Ci (k=response factor)

Question 14

Internal Standards benefit

Answer 14

Accounts for experimental loss

Question 15

What percent of Gaussian is within 1 standard deviation

Answer 15

68.3

Question 16

What percent of Gaussian is within 2 standard deviation

Answer 16

95.5

Question 17

What percent of Gaussian is within 3 standard deviation

Answer 17

99.7

Question 18

Case 1 T-Test

Answer 18

Experimental vs Measured value

Question 19

Case 2 T-Test

Answer 19

Experimental vs Experimental (different samples)

Question 20

Case 3 T-Test

Answer 20

Experimental vs Experimental (SAME samples)