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1

what does work do

work creates order. it lowers the entropy of the system

2

energy is always

converted when work is done. energy is degraded as heat in any reaction when work is done so it lowers the work potential

3

second law of thermodynamics

entropy is continuously increasing and energy transformations proceed spontaneously to convert matter from more/less stable to more/less stable forms.

4

positive Gibbs free energy

products have more free energy than reactants. this is endergonic

5

negative Gibbs free energy

products have lower free energy than reactants. this is spontaneous and exergonic

6

endergonic

this is anabolic (stores energy) and may not occur even when enzyme is present because the activation energy is so high

7

exergonic

is catabolic (releases energy) and may occur spontaneously. the negative delta g can be used to do work

8

metabolism

sum of catabolic and anabolic reactions

9

coupling

to accomplish anabolic reactions, you can pair them with an exergonic reaction such that the net delta g is negative

10

combustion vs aerobic respiration

different as the bonds are not broken by heat in combustion. it also is a generalized reaction, there are mayn intermediate steps.

11

why can't you break down glucose all at once

beause then all the energy would release at once and when that happens, a lot of heat is released

12

oxidation

looses electrons and energy. can also transfer protins (H+)

13

reduction

gains electrons and energy

14

glycolysis takes place in

the cytoplasm

15

cleavage why

it becomes an acid here (before it was a sugar). it was also really unstable so it splits

16

substrate level phosphorylation

occurs in glycolysis times 2. yields 4 atp.

17

substrate level phosphorylation process

ATP is formed by transferring a phosphate group directly to ADP from an intermediate. during glycolysis, chemical bonds are shifted around to provide energy required to form ATP.

18

reduction of NAD+

(endergonic maybe) carries electrons around to donate hydrogen to other molecules

19

net yield of glycolysis

2 ATP, 2 NADH + H+, 2 pyruvate

20

glycolysis is limited by

glucose supply and availability of oxidized NAD+ carriers

21

What is NAD+

enzymes use NAD+ as a cofactor for oxidation reactions.

22

energy stored in NADH vs ATP

ATP: you can release it suddenly. it can't be stored
NADH: this can store potential energy for longer periods of time than ATP

23

how does ATP drive an endergonic reaction?

enzymes that catalyze reactions have two binding sites: one for reactant and one for ATP. the ATP site splits the ATP molecule which releases energy allowing the endergonic reaction to occur.

24

ATP synthesis is a ___ reaction

endergonic reaction which requires energy from cellular exergonic reactions

25

oxidative phosphorylation

ATP is synthesized by ATP synthase using energy from a proton gradient. this gradient is formed by high-energy electrons from the oxidation of glucose passing down an electron transport chain. These electrons, with their energy depleted, are then donated to oxygen, hence the term oxidative phosphorylation

26

why don't cells just link the oxidation of glucose directly to cellular functions that require the energy

would be inefficient

27

recycling NAD+

as long as there are food molecules, cell can churn out ATP but it accumulates NADH and depletes the pool of NAD+. A cell does not contain a lot of NAD+so it must recycle it

1) aerobic respiration
2) fermentation

28

pyruvate after glycolysis

aerobic conditions: pyruvate is further oxidized to yield ATP
anaerobic: pyruvate is converted to lactic acid

29

from cytoplasm to the mitochondrial matrix

the 2NADH+H+ gets oxidized by a protein. the electrons get transferred to FAD (turns to 2FADH2). pyruvate also crosses the membrane. this process requires two ATP which is why there are usually 38 ATP in prokaryotic cells since they don't have membranes to cross

30

glycolysis reactions summary

1) priming reactions use up two ATP to reduce activation energy
2) cleavage occurs and there are 2 G3P (3 carbon phosphates)
3) oxidation and ATP formation NAD+ gets reduced (x2) to add in another phoshate. ATP is formed using substrate level phosphorylation (x2x2) as phosphates are removed

31

Krebs cycle takes place in

the mitochondrial matrix

32

how many electrons does it take to reduce NAD+?

two electrons

33

pyruvate oxidation

link reaction that results in pyruvate being oxidatively dehydrogenated by an enzyme.
1) oxidation of pyruvate produces reduced NAD+ to NADH + H+
2) decarboxylation which produces CO2
3) addition of remaining Acetyl group to coenzyme A through condensation synthesis.

34

after pyruvate oxidation, what happens

pyruvate + NAD+ + coA turns to Acetyl-coA and NADH+H+ + CO2

35

krebs cycle steps

1) condensation: addition of acetyl group through coenzyme A. adds to 4 carbon group and becomes a 6 carbon.
2) first oxidation: oxidation and decarboxylation of the 6 carbon then produces reduced NADH + H+ and carbon dioxide which turning it into a 5 carbon
3) second oxidation: 5 carbon becomes 4 carbon through this process again
4) substrate-level phosphorylation: adding in another coA is a high energy bond. bond is cleaved and the energy is used to produce ATP. still a 4 carbon
5) third oxidation: FAD gets reduced here to FADH2 since the bonds are lower in NADHenergy between this 4 carbon molecule. still stays as 4 carbon
6) regeneration of oxaloacetate: NAD+ gets reduced and 4 carbon original returns

36

Krebs cycle totals

2 pyruvate turns to: 8NADH + H+ + 2FADH2 + 2ATP

2 NADH + H+: 2FADH2

37

Electron Transport chain

1) NADH Dehydrogenase: 8NADH+H+ oxidizes to 8NAD+ and drops of 16 electrons which is used to pump an H+
2) 4FADH2 drops off 8 electrons and becomes 4FAD through oxidation
3) electrons move to bc1 complex (through cytochrome c) and it pumps out another H+
4) cytochrome oxidase complex: uses 24 protons (16 and 8) to reduce oxygen into water.
5) the H+s are stored inside the christae of the intermembrane space, they are looking for a way out since the cytoplasm doesn't have a equilibrium
5) ATP synthase: can be located anywhere in the membrane. due to the above steps, a chemical gradient is created. the outside of the inner mitochondrial membrane is positive due to the H+ ions and the inside is -ve due to the loss of the protons. protons are pushed through the ATP synthase protein which causes the rotor to spin and make ATP by binding together ADP and P.

38

ETC Totals

8NAD+ + 4FAD + 12 H2O +26-32 ATP from oxidative phosphorylation

39

why is it 26-32 ATP

the proton gradient is sometimes not always the same and the process can also be ineffiecient sometimes. Each person's body is different

40

why regulate cellular respiration?

ATP can't be stored for long periods of time

41

metabolic pathway

every step is catabolized by enzymes in a chain reaction

42

enzymes that are regulated

phosphofructokinase and pyruvate dehydrogenase

43

phosphofructokinase

(Noncompetitive- binds to allosteric site)
ACTIVATE: ADP allows more efficient binding of substrate therefore more glucose production
INHIBIT: ATP binds to the allosteric site using energy which means less breakdown and less ATP produced
- high levels of citrate can also inhibit this enzyme

44

pyruvate dehydrogenase

main commitment step in krebs cycle is converting pyruvate to acetyl-coA using dehydrogenase
INHIBIT: high levels of NADH from Krebs cycle competitively binds to this enzyme which stops the exchange of electrons of pyruvate to Acetyl coA. also Acetyl-Coa and ATP
ACTIVATE: NAD+, ADP, Pyruvate

45

what is made when energy stores are low?

acetyl-coA

46

what happens when body has less oxygen

- cause buildup of electrons in ETC due to little oxygen being diminished
- no movement in ETC would mean no oxidized electron carriers (NADH or FADH2) so krebs cycle stops as well as pyruvate oxidation

47

aerobic respiration

needs oxygen, occurs in most cells, makes a lot of ATP, products: CO2, H20 and ATP, occurs in cytoplasm and mitochondria, Reactants: glucose, oxygen, complete combusion

48

anerobic respiration

without oxygen. some organisms have evolved to use other atoms to accept e- for cellular respiration
- the free energy is less since oxygen is more electronegative than other elements
- other atoms have higher bond energy than oxygen does so less energy is released in reaction

49

what do most organisms due in anaerobic respiration conditions

fermentation. the purpose is to use organic molecules to accept the electrons from glycolysis in order to recycle the oxidized NAD+ that is required for process to continue.

Organic molecule + NADH --> reduced organic molecule + NAD+

50

fermentation

in low oxygen conditions, glycolysis still continues while krebs and etc shut down
there are two types

51

alcohol fermentation

- yeast does this
1) glucose goes through glycolysis
2) pyruvate gets decarboxylated (yields CO2) and turns to acetyl aldehyde which turns to ethanol after accepting a pair of electrons from NADH
3) this produces NAD+

52

lactic acid fermentation

- most animals cells do this
1) glucose goes through glycolysis
2) pyruvate turns to lactic acid after accepting a pair of electrons from NADH
3) this produces NAD+

53

Ethanol and lactic acid toxicity

Ethanol: super toxic for yeast
lactic acid: less toxic than alcohol. decreases the pH which denatures enzymes. liver can convert this lactic acid back to glucose later but it takes ATP to break down lactic acid which is why it is called "oxygen debt"

54

energy systems and exercise

depending on type and duration of exercise, different energy systems produce ATP

55

creatine phosphate system

anaerobic
- creatine is made from 3 amino acids
- made in liver and then urinated out
- allows muslce cells to have 15 sec of energy
- stored in skeletal muscles. heart and brain

56

creatine phosphate system process

- bond between creatine and phosphate splits and energy is liberated, through hydrolysis of phosphate bond, the phosphate moves (through substrate level phosphorylation) from creatine to ADP to ATP immediately
- can be stored longer than ATP since its more stable

57

glcolytic or lactic acid system

anaerobic
- basically the lactic acid cycle
- supplements moderate to high intensity activity for up to 45 sec or can supplement aerobic ATP generation over long periods of time if exercise levels exceed the areobic capacity but are below maximum
- other sugars can be fed into glycolysis from food
*see gluconeogenesis

58

gluconeogenesis

when glycotic metabolism exceeds oxidative phosphorylation, glycolysis' end product, lactace gets returned to liver. it requires ATP to reproduce glucose from lactic acid which occurs in cori or lactic acid cycle

59

oxidative system

can use many macromolecules to make energy during more moderate activity for long duration
- nucleic acids, proteins, polysaccarides and lipids

60

nucleic acids

break down to nucleotides which feed into Krebs cycle which produces H2O and CO2

61

proteins

anaerobic deamination
- break down into amino acids
- remove the amino group from each acid a
- deaminated amino acid moves to Kreb's cycle as 5 carbon acid or as 3 carbon acid like pyruvate in pyruvate oxidation link reaction
turns into NH3, H20 and CO2 as waste

62

polysaccarides

anaerobic
- broken down to sugars then into glycolysis and cellular respiration which turns into H2O, CO2 waste

63

lipids and fats

aerobic beta-oxidation
- fats are broken down into fatty acids plus glycerol
- enzymes remove the 2 carbon acetyl group over and over until the full acid is converted to acetyl groups
- each acetyl group is then combined with Coenzyme-A to oxidatively form acetyl-coA this goes into the krebs cycle and is oxygen dependent which explains why aerobic exercise burns fat but anerobic does not
H20 and CO2 waste

64

ATP produced per catabolism of fatty acids

x carbon fat

x/2 ATP
3x NADH= this is ATP from oxidative phosphorylation
x FADH2= this is ATP from FADH2

12 carbon
6 ATP
18 NADH = 54 ATP
12 ATP from FADH2
TOTAL: 72 ATP

65

fats vs carbohydrates energy

fats have 20% more energy than glucose. 1g of fatty acid contains 2x as much energy than 1g of glucose. fats are not metabolized since they take a long time

fats require more oxidation to break down than carbohydrates, thus yielding more energy but at a slower rate.

66

bar graph vs scatter plot

bar graph is comparing things and numerical values (ie dormant vs germinated respiration) while scatter plot is one thing measured over a range of other numerical values (i.e temperature and rate)

67

CO2 and rate of respiration

More waste product means higher “rate of reaction” and more glycolysis occurring.

68

germination and rate of respiration

germinated have higher CO2 since they require a lot of ATP to access the nutrient stored in the endosperm and kick-start the initial root and stem growth

dormant require barely any CO2 as they are not growing and don’t require as many nutrients and ATP as germinating seeds.

69

temperature and rate of respiration

At lower temperatures, both the kinetic energy of the molecules is lower and the seed also wants to produce ATP at a slower rate. The enzymes that catalyze cellular respiration won’t work as efficiently as they are significantly below their optimum range. This allows the seed to conserve its energy, which is a simulation of what would occur during winter.

the higher the temperature, the higher the rate of reaction. This is because the kinetic energy of the system allows the reaction rate to increase as the molecules collide with each other so much more frequently. Also, the enzymes that catalyze the reactions of respiration have an ideal temperature at which they perform.

70

During aerobic respiration oxygen gas is consumed at the same rate as carbon dioxide gas is produced. In order to provide accurate measurements of oxygen consumption, what should the experimenter ensure that they do? (2A)

Include a device that removes CO2 from the volume of the container

71

why would an organism have higher CO2 rate at lower/higher temperatures than in other places

they have to maintain both inner body temperature and do cellular respiration so more ATP

72

in a lab

mention
- equipment used
- removal or waste products that you are not measuring
- include a control with nothing in it
- controlled variables
- independent and dependent variables
- repeat a few times for accuracy

73

pH and co2

an optimum range for the enzymes in cellular respiration and outside of that range it would denature the enzyme and lower the rate anything to that effect would be acceptable.

74

ATP yield totals

Glycolysis: 2 ATP, 2NADH turns to 6 ATP so 8 ATP
oxidation of pyruvate: *2NADH turns to 6 ATP
Krebs: 2 ATP, 6NADH turns to 18 ATP and 2FADH2 turns to 4 ATP

TOTAL: 38 net ATP
* In eukaryotes, 4 ATP is produced here

75

if the ability of cellular respiration is diminished

a cell would need to metabolize more fuel to achieve the typical ATP yield, in essence, raising the cells metabolism, storing less carbohydrates and fats, which potentially results in weight loss.

76

feedback inhibition:

cell produces more quantity of product through a biochemical pathway, the buildup of that product will inhibit the activity of that product's production

77

when pH changes a little (product and substrate)

there is less product produced and more denatured enzymes so the rate of disappearance of the substrate is decreased!

78

a process common to all living prokaryotes and eukaryotes, aerobic and anaerobic is

glycolysis

79

a single glucose molecule can drive the Krebs cycle

2 turns

80

NADH and FADH2 are

coenzymes

81

FADH and NADH comparison:

FADH is lower in energy since it can only pump 2 H+ vs NADh which can pump 3

82

which one is organic, coenzymes or cofactors?

coenzymes

83

which one is inorganic, coenzymes or cofactors?

cofactors

84

in an anabolic reaction,equilibrium will favour

reactants over products

85

free energy

what remains after the amount of energy due to the degree of disorder of the system is subtracted from the difference between the bond energy of the reactants and products

86

second law of thermodynamics (mutiple choice thingy)

order spontaneously goes to disorder

*spontaneously does not mean quickly

87

location of the ATP synthase protein

inner membrane

88

Krebs cycle location

matrix

89

Formation of pyruvate location

cytoplasm of cell

90

electron transport location

inner membrane

91

Lowest pH location

intermembrane space. This is due to the electron transport chain and the large concentration of H+ ions

92

pyruvate oxidation location

matrix

93

many of the proteins in the ETC ARE ____ which incorporate an iron atom in haeme prosthetic group

cytochromes

94

anaerobic

without oxygen. uses a respiratory transport chain but does not use oxygen as the electron acceptors, mostly in prokaryotes, less ATP, Products: CO2, reduced species, ATP, occurs in cytoplasm and mitochondria, Reactants: glucose, electron acceptor (not oxygen), incomplete combustion and produces either ethanol or lactic acid

95

what would happen to muscles under prolonged anaerobic conditions?

lactic acid fermentation

96

38 vs 36 ATP

38: Prokaryotes make 2 more ATP as, in the pyruvate oxidation stage, it takes two ATP to transfer across the membrane therefore 36 net ATP

97

H

total energy of the system

98

TS

absolute temperature times the degree of disorder. represents the degraded energy

99

catabolic example

releases energy so respiration and digestion

100

anabolic example

protein synthesis and photosynthesis

101

in a redox reaction, electrons move from a ___ energy state to a ___ energy state.

higher, lower. this is because electrons are at a higher energy level when they are associated with less electronegative atoms (such as C or H) and at a lower energy level when they are associated with a more electronegative atom (such as O)

102

H+ gradient and chemiosmosis

H+ stored in intermembrane space have a force called 'proton motive force' since they have both high concentration and high charge and their movement can be a form of "potential energy". They find ATP synthase which allows the protons to move and create ATP

103

cash

exchange goods and services, draw out of bank, ATP

104

investments

longer form of energy storage, lipids

105

checking account

glucose

106

saving account

longer period, used to top up checking glycogen

107

why can't energy be stored in NADH or FADH2

they are large expensive molecules to make so it can't be stored for long

108

how does coupling help in cellular respiration

oxidation of glucose releases free energy which can be used as the activation energy for reducing ADP.

109

coupling reaction example

sodium potassium pump: It’s energetically unfavorable to move sodium (Na+ ) out of, or potassium (K+) into, a typical cell, because this movement is against the concentration gradients of the ions. ATP provides energy for the transport of sodium and potassium by way of a membrane-embedded protein called the sodium-potassium pump (Na+/K+ pump).

110

how to draw a reaction graph

y: free energy of system
x: reaction progress

111

noncompetitive inhibition

binds to the allosteric site on enzyme (not denatured, only temporarily changed)