Unit 3 Cellular Energetics 12-16% Flashcards
(51 cards)
Metabolism
All the chemical reactions that occur within an organism.
Catabolic Pathways
Break down complex molecules into simpler components, releasing energy.
Anabolic Pathways
Use energy to synthesize complex molecules from simpler ones.
Function of Enzymes
Speed up chemical reactions without being consumed.
Ex. Lactase catalyzes the hydrolysis of lactose into glucose & galactose.
All chemical reactions that involve breaking & forming bonds require ________.
Energy
Activation Energy
Energy needed to start a chemical reaction.
Enzyme Effect on Activation Energy
Lower the activation energy required for a reaction, making it occur at a faster rate.
Enzymes Specificity
Enzymes typically catalyze only one specific type of chemical reaction.
Substrate
Specific reactant that an enzyme acts upon.
Ex. In the reaction catalyzed by lactase, lactose is the substrate.
Active Site Binding
Substrate binds to the enzyme’s active site.
Induced Fit Model
Enzyme’s active site changes shape upon substrate bonding → more precise fit & enhanced catalytic activity.
Conversion & Release of Products
Substrate is transformed into the products of the reaction → released from the active site.
Reusability of the Enzyme
After the products are released, the active site becomes available for new substrate molecules to bind.
Effect of Temperature on Enzyme Activity
Higher temperatures increase collisions between substrates & enzymes → increased reaction rate.
Excessively high temperatures lead to denaturation where the enzyme loses its shape & becomes inactive.
Effect of pH on Enzyme Activity
Deviation from optimal pH range causes denaturation.
Effect of Substrate Concentration on Enzyme Activity
Higher substrate concentrations increase the reaction rate until the enzyme is saturated (all active sites are occupied) → max rate of reaction.
How to increase the rate of reaction in high substrate concentrations?
Add More Enzymes
Effect of Enzyme Concentration on Enzymatic Activity
Higher enzyme concentration will increase the reaction rate until all of the substrate is bound → levels off.
How to increase the rate of reaction in high enzyme concentrations?
Add More Substrate
Competitive Inhibitation
Inhibitor binds to the enzyme’s active site, preventing the substrate from binding.
Noncompetitive Inhibition
Inhibitor binds to a different part of the enzyme (allosteric site)
causing a conformational change.
Law of Conservation of Energy
Energy cannot be destroyed or created, but is converted from one form to another.
Second Law of Thermodynamics
In every energy transfer, some energy is lost as heat, increasing the overall entropy of the universe.
Entropy
Measures the disorder/randomness of a system, and the amount of energy that is unavailable for doing work.