unit 5 - gram staining

Question 1

what is in the cell wall of a protein that is stained purple in gram staining

Answer 1

peptidoglycan / murein

Question 2

what is ethanol’s role in gram staining

Answer 2

removes outer slime capsule because it breaks down lipids

Question 3

what is the differences between gram negative and gram positive bacteria

Answer 3

gram + has a thick peptidoglycan layer
gram - has a thin peptidoglycan layer but a thick slime capsule

Question 4

what is a restriction enzyme

Answer 4

cleaves DNA sequences at sequence specific sites, producing DNA fragments with a known sequence at each end