Unit 5 Kahoot

Question 1

What exists as normal flora of the mucous membranes in the upper respiratory and urogenital tracts?

Answer 1

Neisseria Species

Question 2

What is the microscopic morphology of N. gonorrhoeae?

Answer 2

Gram negative
Intracellular diplococci
Kidney bean shaped

Question 3

What is not considered normal biota because it is always pathogenic?

Answer 3

Neisseria gonorrhoeae

Question 4

What kind of swabs do we use for N. gonorrhoeae?

Answer 4

Dacron/Rayon
Avoid inhibition of growth

Question 5

What are the symptoms of disseminated gonococcal disease?

Answer 5

Purulent Arthritis
Fever
Rash
Intermittent bacteria

Question 6

What can untreated cervicitis lead to in women?

Answer 6

Pelvic Inflammatory Disease (PID)

Question 7

How is Ophthalmia neonatorum acquired?

Answer 7

Through vaginal delivery
Birth canal infected with N. gonorrhoeae

Question 8

Transgrow, JEMBEC, Bio-Bag, Gono-Pak, and Amies medium are examples of what?

Answer 8

Transport media for N. gonorrhoeae

Question 9

How does N. gonorrhoeae appear on a modified Thayer-Martin (MTM) agar?

Answer 9

Raised, small, gray to tan translucent colonies

Question 10

What temperature must N. gonorrhoeae be incubated at?

Answer 10

35C
3% - 5% CO2

Question 11

What is defined as commensal flora of carriers, found in mucosal surfaces of the nasopharynx and oropharynx?

Answer 11

N. meningitidis
Also recovered from rectal, urogenital and oral-genital sites

Question 12

What are the virulence factors for N. spp.

Answer 12

Capsule (N. meningitidis)
Pili (fimbriae)
Cell membrane proteins
IgA protease

Question 13

What is the presumptive identification of N. meningitides based on?

Answer 13

Colony morphology
Microscope morphology
Oxidase test

Question 14

Who is at increased risk of N. meningitidis?

Answer 14

Crowded living situations
Military recruits
College students

Question 15

What is defined as an opportunistic pathogen that causes upper respiratory infections in otherwise healthy children and the elderly?

Answer 15

Moraxella catarrhalis
Normal commensal of respiratory tract

Question 16

What are the symptoms of meningococcal meningitis?

Answer 16

Fever/headache
Stiff neck
Confusion
Photophobia

Question 17

What specimens are used for N. meningitidis?

Answer 17

CSF
Blood
Nasopharyngeal swabs

Question 18

What is the microscopic morphology of N. meningitides?

Answer 18

Intracellular AND extracellular
gram negative diplococci

Question 19

What can be used to differentiate N. lactamica and N. meningitides?

Answer 19

ONPG test (o-nitrophenyl-B-Dgalactopyranoside)

Question 20

What are amoxicillin, spectrum cephalosporins, TST, azithromycin, and quinolones classified as?

Answer 20

Treatment methods for M. catarrhalis

Question 21

What is the colony morphology of M. catarrhalis?

Answer 21

Hockey puck
Older colonies are “wagon wheels”
smooth, opaque gray to white

Question 22

What are the specimen collection sites for M. catarrhalis?

Answer 22

Middle Ear Effusion
Nasopharynx
Sinus, sputum, bronchial

Question 23

How can we identify M. catarrhalis?

Answer 23

Oxidase and Catalase positive
Asaccharolytic
positive DNase

Question 24

Under what circumstances does M. catarrhalis grow the best?

Answer 24

SBA or Chocolate agar
Tolerates lower temperatures (28C)

Question 25

What are the nutritional factors that Haemophilus requires?

Answer 25

X factor: hematin V factor: NAD

Question 26

Which Haemophilus strain is rarely associated with endocarditis?

Answer 26

H. parainfluenzae

Question 27

Which Heamophilus strain can cause contagious conjunctivitis and is also known as Koch-Weeks bacillus?

Answer 27

Heamophilus aegyptius Pinkeye

Question 28

What is the colony morphology of Heamophilus influenzae?

Answer 28

Translucent, moist, smooth, convex Mousy or bleach odor

Question 29

What can Cefotaxime/ceftriaxone, azithromycin, and sulfamethoxazole be grouped into?

Answer 29

Treatment options for Heamophilus infections

Question 30

What are the members of the HACEK group?

Answer 30

H: Aggregatibacter aphrophilus Actinomycetemcomitans Cardiobacterium hominis Eikenella corrodens Kingella spp

Question 31

What is often transmitted to humans by a dog bite or cat scratches?

Answer 31

Pasteurella spp

Question 32

What is the most commonly isolated Pasteurella spp?

Answer 32

P. multocida

Question 33

What temperature do we incubate Heamophilus influenzae at?

Answer 33

33 - 37C 5% - 10% CO2

Question 34

What are the symptoms of H. ducreyi?

Answer 34

Painful lesions of the genital and perianal areas Penis/labia or vagina

Question 35

What do we find in each quadrant of a Heamophilus quad plate?

Answer 35

Q1: X factor (Hemin) Q2: V factor (NAD) Q3: X & V (Hemin/NAD) Q4: X & V with horse blood

Question 36

Which fastidious organisms are chocolate agar essential for recovery and presumptive identification?

Answer 36

H. influenzae A. aphrophilus C. hominus

Question 37

What can we perform the Porphyrin test on?

Answer 37

Agar Broth Disk

Question 38

In the Porphyrin test, which technique is positive showing a fluorescent reddish orange under UV light in the presence of porphryins?

Answer 38

Wood's ultraviolet (UV) lamp

Question 39

Which member of the HACEK group appears as yellow, granular, convex colonies with opaque centers?

Answer 39

A. aphrophilus Chocolate agar

Question 40

What is also known as rabbit or deerfly fever?

Answer 40

Francisella tularensis Handle in BSL3

Question 41

What does Legionella pneumophilia cause?

Answer 41

Community acquired pneumonia

Question 42

What can azithromycin and fluoroquinolone be used to treat?

Answer 42

Legionella pneumophilia

Question 43

What methods can be used to identify L. pneumophilia?

Answer 43

Direct Fluorescent antibody test (DFA) DNA detection Serologic testing Urine antigen testing

Question 44

What is the microscopic morphology of L. pneumophilia?

Answer 44

Thin, weakly staining, gram negative rods Pleomorphic

Question 45

What are the specimens of choice for L. pneumophilia?

Answer 45

Bronchoalveolar lavage Sputum Bronchial washing

Question 46

What is the definition of Legionnaires' Disease?

Answer 46

A febrile disease with pneumonia caused by L. pneumophilia

Question 47

What is a febrile disease without pneumonia involvement caused by L. pneumophilia?

Answer 47

Pontiac Fever

Question 48

What must we do to a L. pneumophilia specimen if there is more than a 2 hour delay?

Answer 48

Refrigerate if more than 2 hours Freeze at -70C if several days

Question 49

Why is serological testing for Bordetella pertussis not commonly used?

Answer 49

Not standardized Not widely available Expensive

Question 50

What is the selective media for culture of B. pertussis?

Answer 50

Bordet-Gengou potato infusion agar Glycerol and sheep blood

Question 51

What does B. pertussis/parapertussis cause?

Answer 51

Whooping cough 3 stages

Question 52

How do we collect a specimen for B. pertussis?

Answer 52

Nasopharyngeal aspirates/swabs Rayon or Dacron swabs used