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medical science > Unit2 safety > Flashcards

Unit2 safety Flashcards

1
Q

go to the notes and label the different hazard symbols

A

Answer on notes

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2
Q

Through which 2 organs or tissue can one transmit infections in the lab

A

Through the skin and conjuctiva (eye mucous membrane)

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3
Q

Which one between the eye and intact skin is an effective barrier in preventing transmission

A

Intact skin

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4
Q

What are the 7 things to do to avoid 💉 infection transmission through the eyes and skin

A

1.wash hands frequently
2.avoid 💦 splashing
3.avoid touching eyes
4.never apply make-up
5.dispose off waste correctly
6.prevent contact of skin with contaminated material
7.use safety goggles.

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5
Q

What 4 ways help us to avoid infection through the mouth

A

1.never mouth pipette
2.avoid touching mouth
3.dont eat, drink or smoke
4.never apply lip moisturizer

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6
Q

What two ways is infection transmitted through the respiratory tract

A

1.aerosols and 2.infective dusts

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7
Q

How can we avoid 1.aerosols and 2.infective dusts

A
  1. Don’t break or drop tube with broth culture
    2.dont open free-fried cultures and fungal culture
    3.use safe and gentle techniques
    4.allow wire loop to cool
    5.clean up spills immediately
    6.centrifuge in sealed buckets
    7.open tubed in safety cabinets.
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8
Q

List 10 lab facilities requirements

A

1.no overcrowding
2.hand basins and disposable towels
3. Safety officer
4. Separate room for records
5.disinfectants - Sodium hypochlorite
6.proper animal facilities
7.safety cabinet
8.smooth impermeable benches
9.sealed buckets for centrifuges
10.protective materials available

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9
Q

What is the safety code for cuts

A

Wash with soap and water

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10
Q

What is the safety code for eye

A

Rinse with eye bath (saline solution) for atleast 20min

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11
Q

What is the safety code for mouth

A

Spit and rinse well with water

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12
Q

What is the safety code for skin

A

Wash with disinfectant and then with soap and water

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13
Q

What is the safety code for clothes

A

Rinse with disinfectant then with soap and water, can also autoclave (sterilization method that uses high pressure steam)

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14
Q

What is the safety code for spills

A

Pour spillage with disinfectant, leave for 10min and wipe with disposable towel

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15
Q

What are the 3 NO’s

A

1.No eating 🍲 smoking 🚬 drinking 🍻
Make up💄
2.No licking 😝
3. NO fingering eyes👀

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16
Q

What are the 5 DO’s

A

1.Do wear PPE
2.Do wash hands before leaving the lab
3.Do remove laboratory before leaving lab
4.Do dispose waste correctly
5.Do wash hands after any procedure that result in possible contamination.

17
Q

3 rules regarding specimens

A
  1. Discard leaking specimens
  2. Open respiratory pathogens containing specimen in safety cabinet
  3. Open containers slowly to avoid splashing and aerosols
18
Q

In which 5 ways can we care for our workplace (lab)

A

1.disinfectants and bins should be visible
2.keep it tidy
3.keep tunes and containers in racks
4.wipe benches with disinfectant before and after a session
5.replace previous day’s jars with new ones with fresh disinfectants.

19
Q

What are the 5 bench techniques

A

1.avoid splashing and spilling
2.discard items correctly
3.work gently
4.when flame 🔥 splatters, place loop in boiling water /disinfectant or safety cabinet

20
Q

How does putting the flame in a safety cabinet help

A

It prevents aerosols from being breathed in.

21
Q

What goes into the safety cabinet

A

TB and other airborne (air based) pathogens

22
Q

What are the 4 rules of using a safety cabinet

A
  1. Switch off UV light and turn on ventilating fan before use.
    2.correctly disinfect area inside the cabinet before and after use
  2. After use switch on UV lamp
    Lastly ensure cabinet is serviced regularly.
23
Q

What are the 5rules regarding care of the workplace

A

1.disinfectants and bins should be visible
2.keep tubes and containers in racks
3.wipe benches with disinfectant before and after the lab
4.replace with fresh disinfectant everyday.

24
Q

What are the 5 rules of bench technique

A

1.avoid splashing
2.discard items correctly
3.work gently
4.when flaming 🔥 are likely to splatter, put them in boiling water, disinfectant or safety cabinet.
5.no mouth pipetting

25
Q

What are kept on a safety cabinet

A

TB specimens and other airborne pathogens

26
Q

What is the first things we do before using a safety cabinet

A

1.switch off UV light and turn on ventilating fan before use

27
Q

List the 3 remaining rules of using safety cabinets

A

2.correctly disinfect area inside cabinet before and after use.
3.after use switch on UV lamp
4. Ensure cabinet is serviced regularly

28
Q

What do we use to check airflow in a safety cabinet

A

HEPA filter

29
Q

What are the 4 rules regarding centrifuge use

A

1.balance loads
2.open when centrifuge comes to complete stop
3.if it breaks allow to stand for 10min
4.disinfect

30
Q

True or false
We should allow centrifuge to come to stop fast because we rushing for physics period

A

False.
We should allow it to come to stop slowly.

31
Q

What are the 4 centrifuge rules regarding tubes

A

1.use centrifuge safe tunes only
2.use tubes with lids and ensure properly closed.
3.dont overfill tunes
4.ensure tube fits all the way down in bucket.

32
Q

List 5 diseases you should be immunized for to work in the lab

A

1.TB
2.Polio
3.Tetanus
4.Diphtheria
5.Typhoid
6.Rubella
7.Hepatitis A and B

33
Q

How do we clean and prepare the following…
New glass

A

1%HCL, Wash in tap water, rinse in distilled water and sterilize

34
Q

Pippetes

A

3%lysol or rinse in tap water, sterilize in hot air oven or use disposable plastic pippetes

35
Q

General lab glassware

A

3% lysol or boiing soap solution

36
Q

What is the function of a centrifuge and the two types

A

A centrifuge separates particles of different densities
1.Swing out rotor (horizontal sedimentation)
2.Angle rotor (angle sedimentation)

37
Q

List 5ways of care and maintenance of centrifuge

A

1.position centrifuge on firm base
2.wipe interior bowl frequently with disinfectant
3.

38
Q

List 5ways of care and maintenance of centrifuge

A

1.position centrifuge on firm base
2.wipe interior bowl frequently with disinfectant
3.replace carbon brushes periodically
4.prevent aerosols by using capped tubes or place in safety cabinets
5.examine bucket frequently for flaws.

medical science (6 decks)

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