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QC2 > UV VIS > Flashcards

UV VIS Flashcards

(64 cards)

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1
Q

the study of the absorption and emission of light and other radiation by matter

A

SPECTROSCOPY

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2
Q

the measurement of the interactions between light and matter, and the reactions and measurements of radiation intensity and wavelength

A

SPECTROMETRY

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3
Q

the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength

A

SPECTROPHOTOMETRY

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4
Q

this is when particles are moved from their normal room temperature state, or ground state, to one or more higher-energy excited states.

A

ABSORPTION OF RADIATION

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5
Q

normal room temperature state

A

ground state

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6
Q

is produced when excited particles (atoms, ions or molecules) relax to lower energy levels by giving up their excess energy as photons

A

electromagnetic radiation

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7
Q

electromagnetic radiation is produced when excited particles (atoms, ions or molecules) relax to lower energy levels by giving up their excess energy as

A

photons

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8
Q

causes of emission

A

(1) bombardment with electrons or elementary particles, which leads to emission of X-radiation
(2) exposure to an electric current or intense heat source producing UV, visible or IR radiation
(3) irradiation with a beam of electromagnetic radiation
(4) an exothermic chemical reaction that produces chemiluminescence

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9
Q

principle of uv vis

A

irradiation with a beam of electromagnetic radiation

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10
Q

this is when electromagnetic radiation is transmitted at all angles from the original path; usually observed when atomic or molecular particles are small relative to the wavelength of the radiation

A

SCATTERING

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11
Q

a parallel beam of radiation is bent as it passes by a sharp barrier or through a narrow opening

A

DIFFRACTION OF RADIATION

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12
Q

type of energy that is commonly known as light

A

ELECTROMAGNETIC RADIATION

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13
Q

aka white light

A

ELECTROMAGNETIC RADIATION

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14
Q

ELECTROMAGNETIC RADIATION is propagate in 2 fields known as

A

Electric and Magnetic Field Oscillation

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15
Q

ELECTROMAGNETIC RADIATION (PROPERTIES) Models

A

sinusoidal wave model
particle model

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16
Q

distance from basic to highest point

A

amplitude

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17
Q

time in sec where 2 minima passes through the same
point

A

period

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18
Q

no. of oscillators in sec

A

frequency

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19
Q
  • distance between 2 successive crest/trough
A

wavelength

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20
Q

reciprocal of wavelength

A

wavenumber

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21
Q
  • electromagnetic radiation transport into a
A

speed of light

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22
Q

wavelength and frequency

A

indirect

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23
Q

wavelength and energy

A

indirect

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24
Q

frequency and energy

A

direct

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25
constant value of speed of light
3.00x108 m/s
26
The energy of a photon absorbed or emitted during a transition from one molecular energy level to another is given by the equation:
QUANTUM Theory
27
Study of discrete particles
QUANTUM THEORY
28
Planck’s constant
6.62x10-34 Js
29
1 electron volt (eV) =
1.6 x 10^-19 J
30
1 joule (J) =
10^7 ergs
31
an electronic transition spectroscopic technique in which the interaction between incident radiation and electrons results in the promotion of one or more of the outer or the bonding electrons from a ground state into a higher-energy state
Ultraviolet-visible (UV-Vis) spectroscopy
32
Ultraviolet-visible (UV-Vis) spectroscopy is an electronic transition spectroscopic technique in which the interaction between ____________ and __________ results in the promotion of one or more of the outer or the bonding electrons from a ________________ into a ____________
incident radiation and electrons; ground state into a higher-energy state
33
Radiation in the wavelength range _____________ nm is passed through a solution of a compound.
200–700
34
The electrons in the bonds within the molecule become excited so that they occupy a __________________ and in the process absorb some of the energy passing through the solution.
higher quantum state
35
The more loosely held the electrons are the ________ the wavelength
longer
36
extended system of double bonds that causes absorption of light at UV-visible region
CHROMOPHORES
37
CONTINUUM SOURCE (continuous)
tungsten halogen for visible deuterium arc for UV xenon arc for both
38
CONTINUUM SOURCE (pulsed)
xenon flash lamp
39
used to disperse the light into its constituent wavelengths, which are further selected by the slit
MONOCHROMATOR
40
is rotated so that a range of wavelengths is passed through the sample as the instrument scans across the spectrum
MONOCHROMATOR
41
have multiple exit slits, each of which allows a different wavelength to pass through it
POLYCHROMATOR
42
conventional rectangular cells fiber-optic-based immersion probes automated sample changers micro-well plate configurations flow cells
Sampling area
43
generate an electric current that is directly proportional to the intensity of the radiant energy incident upon them
Photoelectric detectors
44
photosensitive semiconductor devices photomultipliers
detector
45
DIODE-ARRAY INSTRUMENTS HPLC DETECTORS FIBER-OPTIC-BASED MODULAR SYSTEMS
ALTERNATIVE DETECTOR CONFIGURATIONS
46
the optical configuration is reversed from that in a conventional spectrophotometer, and the light beam passes through the sample before being dispersed by the polychromator
DIODE-ARRAY INSTRUMENTS
47
gives the benefit of fast, full spectral data with no moving parts that can wear out
DIODE-ARRAY INSTRUMENTS
48
high-stability, low signal-to- noise ratio output at high transmittance levels through a small-aperture flow cell
HPLC DETECTORS
49
flexible and easy to use allow measurements to be performed on micro- plates customized systems
FIBER-OPTIC-BASED MODULAR SYSTEMS (adv)
50
there might be room light interference custom-built systems do not have additional shuttering, stray light filtering light levels transmitted directly down fibers from high-intensity sources such as Xenon flash lamps may cause photodegradation
FIBER-OPTIC-BASED MODULAR SYSTEMS (dis)
51
the width of the band of light at one-half the peak maximum (or full width at half maximum
SPECTRAL BANDWIDTH
52
is adequate To have an accurate result.
2 nm
53
source of electromagnetic radiation that would be flashed through the sample to know the absorbance and transmittance. May later on affect the resolution (how accurate the data the instrument will produce).
light beam
54
radiant energy at wavelengths other than those indicated by the monochromator setting and all radiant energy that reaches the detector without having passed through the sample or reference solutions
STRAY LIGHT
55
any scattered radiation from imperfections in the dispersing medium light leaks in the system incorrect wavelength calibration incorrect optical alignment reduced source output reduced detector response
STRAY LIGHT - CAUSES
56
Relationship between a specific absorbance and the absorbance of the solution
OPTIMUM WORKING PHOTOMETRIC RANGE
57
is the photometric range in this spectrum of molar absorptivities at different concentrations
center plateau
58
suspended particles scatter light by the __________ effect, causing a ____________ in the measured intensity that __________ as the wavelength decreases
Tyndall; decrease; increase
59
property of light wherein light can be scattered in cases of colloidal solution.
Tyndall effect
60
the concentration of a chemical solution is directly proportional to its absorption of light
BEER’S LAW
61
each layer of equal thickness of the medium absorbs an equal fraction of the energy traversing it
LAMBERT’S LAW
62
SPECTRAL BANDWIDTH STRAY LIGHT OPTIMUM WORKING PHOTOMETRIC RANGE
INSTRUMENTAL FACTORS
63
FLUORESCENCE AND LIGHT SCATTERING
SAMPLE-BASED FACTORS
64
CARE OF CELLS ALIGNMENT AND FILLING OF CELLS CELL CORRECTIONS
SAMPLING FACTORS CONSIDERATIONS

QC2 (6 decks)

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