Venipuncture Collection Tubes

Question 1

general order of draw

Answer 1

1. blood culture tubes (yellow-black)
2. non-additive tube (red)
3. coagulation tubes (light blue)
4. additive tubes (in this order)
   
   • SST (red-grey or gold)
   • sodium heparin (dark green or brown)
   • PST (light green)
   • EDTA (lavender)
   • ACDA or ACDB (pale yellow)
   • oxalate/fluoride (light grey)

Question 2

additive in red top

Answer 2

none

Question 3

MOA of red top

Answer 3

blood clots and the serum is separated by centrifugation

Question 4

uses of red top

Answer 4

chemistries, immunology, serology (blood bank/cross match)

Question 5

additive in gold top

Answer 5

none

Question 6

MOA of gold top

Answer 6

serum separator tube (SST) contains a gel at the bottom to separate blood from serum on centrifugation

Question 7

uses of gold top

Answer 7

chemistries, immunology and serology

Question 8

additive in light green top

Answer 8

plasma separating tube (PST) with lithium heparin

Question 9

MOA of light green top

Answer 9

anticoagulates with lithium heparin; plasma is separated with PST gel at the bottom of the tube

Question 10

use of light green top

Answer 10

chemistries

Question 11

additive of red-gray top

Answer 11

SST with clot activator

Question 12

MOA of red-gray top

Answer 12

forms clot quickly and separates serum with SST gel at the bottom of the tube

Question 13

use of red-gray top

Answer 13

chemistries

Question 14

additive of lavender top

Answer 14

EDTA liquid

Question 15

MOA of lavender top

Answer 15

forms calcium salts to remove calcium

Question 16

uses of lavender top

Answer 16

hematology (CBC) and blood bank (crossmatch)

Question 17

special note for lavender top

Answer 17

requires full draw and inversion 8x to prevent clotting and platelet clumping

Question 18

additive of light blue top

Answer 18

sodium citrate

Question 19

MOA of light blue top

Answer 19

forms calcium salts to remove calcium

Question 20

use of light blue top

Answer 20

coagulation tests (PT and PTT)

Question 21

special note for light blue top

Answer 21

requires full draw

Question 22

additive of dark green top

Answer 22

sodium heparin or lithium heparin

Question 23

MOA of dark green top

Answer 23

inactivated thrombin and thromboplastin

Question 24

uses for dark green top

Answer 24

1. for lithium level: uses sodium heparin

2. for ammonia level: uses sodium or lithium heparin

Question 25

additive of dark blue top

Answer 25

sodium EDTA

Question 26

MOA of dark blue top

Answer 26

tubs is designed to contain no contaminating metals

Question 27

uses of dark blue top

Answer 27

trace element testing (zinc, copper, lead and mercury) and toxicology

Question 28

additive in large gray top

Answer 28

sodium fluoride and potassium oxalate

Question 29

MOA of large gray top

Answer 29

actiglycolytic agent preserves glucose up to 5 days

Question 30

use of large gray top

Answer 30

1. for lithium level: uses sodium heparin | 2. glucose level requires full draw (hemolysis id short draw)

Question 31

additive of yellow top

Answer 31

ACD (acid-citrate-dextrose)

Question 32

MOA of yellow top

Answer 32

complement activation

Question 33

use of yellow top

Answer 33

HLA tissue typing, paternity testing, DNA studies

Question 34

additive in yellow-black top

Answer 34

broth mixture

Question 35

MOA of yellow-black top

Answer 35

preserves viability of microbes

Question 36

use of yellow-black top

Answer 36

microbiology - aerobes, anaerobes and fungi

Question 37

additive of black top

Answer 37

sodium citrate (buffered)

Question 38

MOA of black top

Answer 38

forms calcium salts to remove calcium

Question 39

uses of black top

Answer 39

Westergren Sedimentation rate

Question 40

special note for black top

Answer 40

requires full draw

Question 41

additive in orange top

Answer 41

thrombin

Question 42

MOA of orange top

Answer 42

quickly clots blood

Question 43

use of orange top

Answer 43

STAT serum chemistries

Question 44

additive in brown top

Answer 44

sodium heparin

Question 45

MOA of brown top

Answer 45

inactivates thrombon and thromboplastin

Question 46

use of brown top

Answer 46

serum lead determination