WBC counting

Question 1

Normal range of WBC

Answer 1

4000 – 11,000 / μL

Question 2

Türk solution contents and facts

Answer 2

Methylene blue dissolved in Acetic acid
Causes lysis of RBCs and stains WBC nuclei blue

Question 3

Blood collection steps

Answer 3

1) Patient wash hands (warm water)
2) Disinfect with alcohol on cotton swab
3) Open needle (twist & pull)
4) Discard in yellow bin
5) After prick, wipe off first drop (due to interstitial fluid & debris)
6) Pipette 10μl of fresh blood
7) Apply new paper on wounded fingertip

Question 4

Steps for lab prep

Answer 4

1) Pour out Türk solution into another beaker
2) Pipette 90μL into eppendorf tube
3) Obtain blood and pipette 10μL from the finger into Türk solution
4) Mix for 5-10 sec
5) Pipette 20μL from mixture into Bürker chamber

Question 5

How to count WBCs

Answer 5

1) Count visible cells in 25 large squares
2) That number is per 0.1μl
3) Take final number (e.g. 60) and multiply by 10 and 10 again
4) 60 x 10 x 10 = 6000 /μl

Question 6

What to do if some cells are on the edge of the line?

Answer 6

Count cells touching 2 of the lines and use the same 2 lines for all the boxes