Week 1 Inflammation Flashcards
Blood cells involved in inflammation
Neutrophils, Eosinophils, Basophils, Monocytes, Lymphocytes, Platelets,
ENDOTHELIAL cells, basement membrane, and Smooth Muscle cells.
Connective tissue cells involved in inflammation
Mast cells, Fibroblasts, Macrophages, Lymphocytes
Plasma proteins involved in inflammation
Coagulation proteins, Kinins, Fibrinolytic enzymes
Duration and main characteristics of Acute Inflammation
1 week max.
Edema fro increased blood flow and vascular permeability, exudate.
Leukocyte emigration to site of inflammation
Rubor, Tumor, Calor, Dolor
Redness, Swelling, Heat, Pain
Loss of function
What are the advantages of exudation to a wound site?
Dilution of toxins
Increased protein content –> Increased Immunoglobulin
What are the advantages of exudation to a wound site?
Dilution of toxins
Increased protein content –>
- Increased Immunoglobulin
- Fibrin nets, fixing bacteria and wound healing
- Acute phase proteins
What are 4 methods of vascular leakage
Endothelial cell contraction in small venules - histamine, bradykinin, leucotrienes.
Occurs rapidly and is short lived.
Junctional retraction - IL-1, TNFa, IFNy
-delayed effect after 4-6 hours.
Leokocyte-dependent leakage
- leukocyte-produced proteolytic enzymes and free radical increase permeability
Leakage from regenerating/damaged capillaries
-increased leakage as angiogenesis occurs and vessels aren’t sealed tightly.
Stages and mediators of extravasation
1) Rolling. P-selectins bind endothelial glycoproteins
P-Selectin on leukocytes — P-selectin glycoprotein ligand-1 on Endothelial cells
VLA-4 also
2) Slow rolling. Shorter, higher affinity E-selectins bind glycoproteins
E-Selectins
3) Slow rolling allows for chemokine signaling to increase as they move through the inflamed region
4) Firm Adhesion. Leukocyte Integrins bind endothelial GAGs on endothelial Cell Adhesion Molecules (glycoproteins).
VLA1 —- VCAM1
(Very Late Antigen 1)
LFA1 —- ICAM1
(Leukocyte Function-associated Antigen 1)
5) Extravasaion/Diapdesis
Also involves cell adhesion molecules and integrins: LFA1, VCAM1, ICAM1, ICAM2
Exogenous substrates (from pathogens) that activate leukocytes
PAMPs and DAMPs, bound by C-type lectins, and TLRs TLR1, 2, 4, 5, 6, which can be Endosomal or Cell Surface receptors
Pathogenic associated molecular patternsm, PAMPs:
From GrPos bact: G+ cell wall peptidoglycan, lipoteichoic acid
From GrNeg bact: LPS
From either: Flagellin, N-formylmethionine, hypometheylated CpG-DNA, free single or double stranded DNA or RNA
Damage associated molecular patterns, DAMPs:
Components of cells that should be intracellular but are encountered by the macrophages in the environment (from lysed/dead cells)
Nuclear proteins
Mitochondrial DNA
Oxygen radicals
Heat shock proteins
What are endogenous substrates that activate leukocytes?
IL-8
Lipoxygenase productes LTB4
Complement proteins C5a, C3a
What is the receptor expressed on phagocytes that binds opsonized microbes and induces phagocytosis?
What is its ligand?
Receptor: Fc-gamma receptors FcyRs
Ligand: C3b and Fc-fragment of IgG in complex
What are the two pathways of that can initiate phagocytosis?
Opsonin mediated pathway: binding opsonized, antibody bound microbes
Nonspecific pathway: PAMPs
What are the stages of phagocytosis?
What are the two major pathways used to kill target microbes?
1) Recognition and binding
binding PAMPS or opsonized antibody complexes
2) Englufment
Surrounding, Fusion of phaocytic and lysosomal granules onto the target microbe membrane
3) Killing Oxygen dependent: NAPDH oxidase --> Superoxide ion --> H2O2 MPO --> H2O2 + Cl- --> HOCl NO synthase --> Peroxinitrite
Oxygen indepdendent lysozymes lactoferrin MBP, Major basic protein Defensins - pore forming proteins Acid hydrolase
What are some types of Acquired Defects in leukocyte functions? ie, what can disrupt chemotaxis, phagocytosis, microbe killing ability
Burns, Malignancy, Sepsis, AIDS –> disrupt chemotaxis
Hemodialysis –> disrupts leukocyte adhesion
Leukemia, Anemia, Sepsis, Malnutrition, and Neonatal period –> disrupts phagocytosis and microbiocidal ability
Diabetes –> disrupts chemotaxis, adhesion, phagocytosis and killing ability
What major inflammatory cytokines initiate the Acute Phase Reaction?
What other major event do these cytokines initiate?
IL-1
IL-6
TNFa
Stimulate Fever, by activating the hypothalamic regions responsible for inducing fever.
What is the Acute Phase Response? (APR)
What can stimulate it?
The changes in protein expression primarily caused by altered expression in the liver, induced by inflammatory cytokines that are released from innate immune cells.
Stimulated by:
Trauma/Injury, Infection, Stress, Neoplasia, or Inflammation
What are the Acute Phase Proteins? (APPs)
CRP: 100-1000x C1 inhibitor: 6-8x Antitrypsin: 2-4x Fibrinogen: 2-4x Complement protein C3 and Factor B: 1.5-2x MBL: Mannose Binding Lectin, activates Lectin pathway of complement system along with MASP Protease inhibitors Ceruloplasmin - Scavenges ROS radicals
Negative acute phase protein:
Transferrin: decreases to limit available iron, which is used by bacteria
Albumin
Antithrombin
What is the function of CRP?
It binds to lysophosphatidylcholine (a phosphatidy-choline with one of its FA tails hydrolyzed off)
This is found on dead/dying cells and on some bacteria
CRP binds the lysophosphatidylcholine, and also binds and activates the C1 complement protein complex, allowing it to activate the classical pathway of the complement system (active C1 generates C4bC2b C3-convertase)
What are the 3 major Plasma Protease systems?
Kinin system
Thrombotic/Fibrinolytic system
Complement system
What are the functions of 1) Bradykinin and 2) Kallinkrein?
Bradykinin:
Arteriole Dilation
Increases Venule permeability
Causes pain in nociceptors
Kallinkrein:
Activates plasminogen –> plasmin
Activates C1q
What are the effects of fever?
What is the beneficial range and pathologic range?
What is the major factor that induces fever?
38-38.5 beneficial
> 39 needs to be brought down.
Bacteria have a narrow temperature optimum, fever may disrupt this
Fever also increases reaction rates, increases immune reactions
IL-6 predominantly generates fever (IL-1 and TNFa to a lesser degree)
What are the effects of fever?
What is the beneficial range and pathologic range?
What is the major factor that induces fever?
38-38.5 beneficial
> 39 needs to be brought down.
Bacteria have a narrow temperature optimum, fever may disrupt this
Fever also increases reaction rates, increases immune reactions.
Each 1 degree C increases Heart rate by 10 bpm.
High temperatures also disrupt body protein function, major organ functions.
IL-6 predominantly generates fever (IL-1 and TNFa to a lesser degree)
What is Hageman Factor?
Coagulation factor XII (12)
Activates Intrinsic Pathway of coagulation (contact of blood with foreign object in blood, other pathway is the TF pathway/extrinsic pathway)
XIIa –> activates XI,
XIa –> activates IX
IXa, VIIa, plus X generates IXa, VIIa, Xa ten-ase complex.
1) Activates Intrinsic Coagulation pathway
2) Activates Kallikrein, which also activates Bradykinin
3) Converts Plasminogen to Plasmin
4) Plasmin can also activate complement proteins C3 and C5 to C3b and C5b.
What are major effects of Plasmin?
Digests fibrin, and causes vasodilation by generating FDP (fibrin degradation products)
Activates complement system C3 -> C3b
