Week 10 - Nidovirales Coronaviridae Arterivirdae Ronivirdae Flashcards by Christine Parascandola

Nido means?

Nest

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Name the viruses that belong to the order Nidovirales.

1- Coronaviridae
2- Arterivirdae
3. Roniviridae

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Name the families that fall under the Order Nidovirales.
What viruses are within the genus Okavirus?

Mentioned families
Okavirus –> GAV, YHV (prawns, shrimp)
Arterivirus
Coronavirus ; now 4 groups of coronaviruses

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Different virus families.

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Know each of these pictures.

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Just shows diversity and complexity of coronaviruses

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Coronaviridae – General Properties
1. Corona: from Latin corona, “_______”; refers to the characteristic appearance of surface __________ that create an image reminiscent of the ______ corona.
2. Enveloped or non-enveloped?, ____-_____ nm
3. **__________-strand RNA (27-32 kb),

____________ replication
__________ into ER and Golgi
Notoriously difficult to propagate in ________
High frequency of ___________**

Coronaviridae – General Properties
 Corona: from Latin corona, “halo”; refers to the
characteristic appearance of surface projections that
create an image reminiscent of the solar corona.
 Enveloped, 120-160 nm
 Positive-strand RNA (27-32 kb), Cytoplasmic replication
 Budding into ER and Golgi
 Notoriously difficult to propagate in culture
 High frequency of recombination

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What virus family is pictured below?

Coronaviridae

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Important
Label accordingly

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Label accordingly

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Causes of variations among CoVs/emergence of new viruses/strains of viruses
* ______ and _____ are major reservoirs for CoVs
Some coronaviruses have ability to
-Change tissue _____
-Pass species ____
-adapt to new ecological _____
Causes of CoV variations
- Accumulation of _____ mutations
- Poor proofreading capability of ______ gene (virus in replication cycle makes errors, which convey into mutations –> development of ____ strains).
- Possibility of ________*

Bats, birds, tropism, barriers, niches, point, RdRP, new, recombination

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Most-up-to-date classification of Coronaviridae
* ICTV: coronavirus group meeting Oct 2020
* Coronaviridae: 2 subfamilies, 5 genera, 26 subgenera and 46 species

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Within the family Coronviridaue, name the Subfamily, Genus, and the Subgenus

Letovirinae, alphaletovirus, milecovirus

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How many groups of Coronaviruses are there?
Name the viruses belonging to each group.
What do the letters on the side mean?
Gamma corona virus is specifically for which species?

4
P = pigs, F = feline, C = canine, etc.
Birds

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Just FYI

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6/7 human coronaviruses, some of which have zoonotic potential

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VERY IMPORTANT

1.What is the length of the coronavirus genome?
2.2/3 of genome is made up of?
3.S =? HE = ?
4.Subgenomic mRNA for S, E, HE; These subgenomes make the nest.

What is the length of the coronavirus genome? 27-31 kb, from 5’ to 3’
unstructeed protein aka gene 1. Divided into two parts: 1a and 1b. There is overlapping between 1a and b called ribosomal frame shifting. Overlpaping frame synthesizes proteins, frame shifts back and moves into another frame in order to synthesis.
S = ?, HE = aquired to come from influenza virus type B; Virus with HE gene is bigger than virus without it.

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Gamma is shortest one: 27.6
Beta is biggest one: 31.3 kb
HE gene is 3-4 kb

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Do not memorize this

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Describe the pathogenesis of Coronaviruses.

Outside the host = inactive
Activated inside the host by host cell proteases (furin)
Respiraotry tract has proteases that cleave spike gkycoprotein, activating the virus.
Drugs that inhibit furin prevent infection from occurring.

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What is the receptor for SARs-COV?
What is the receptor for MERs-COV?

ACE2
DPP4

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Virus by negative pressure –> injected through hole into host cell.

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Coronavirus life cycle

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MERS-CoV life cycle

Human coronaviruses * First described in the 1960s from the nasal cavities of patients common cold named human coronavirus 229E and human coronavirus OC43. * Currently **___** Human coronaviruses are currently known 1- HCoV-229E 2- HCoV-OC43 **3- SARS-CoV** 4- HCoV-NL-63 5-HCoV-HKU1 **6- MERS-CoV 7-HCoV-44 (enteric) ****8- SARS-CoV-2

Evolution of Human Coronaviruses in context of One Health concept

SARS coronavirus- SARS-CoV * **Year _______:** Previously unknown coronavirus was isolated from patients * 8,098 SARS cases worldwide during the 2003 outbreak, resulting in 774 deaths * April 2003, following the outbreak of SARS in Asia & secondary * cases elsewhere in the world * Complete genome sequence April, 2003, : MSFC, UBC, Canada **Intermediate host? ** ______ Delicacy; Minimize human exposure.

2002, Mongoose

What is pictured below? How does this relate to coronaviruses?

SARS-CoV-Intermediate Host-Civet Cat Nocturnal animal related to Mongoose Delicacy

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) -A **novel _________** (nCoV) first reported on 24 September 2012 -Belongs to the genus Betacoronavirus, as does SARS-CoV - Symptoms of MERS - **Severe _______ _________ illness** with symptoms of -Fever, cough, and shortness of breath **-About half of them died **-Some people were reported as having a mild respiratory illness -MERS-CoV spread from person to person

coronavirus, acute respiratory

Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Global distribution of MERS-CoV cases

Classification of MERS-CoV

Renamed novel to proper name

BatMERS-CoV Evolution Theories????

Bat on palm tree --> defecate --> fruit contamined --> human consumption Camels (1 and 2 hump) are reservoirs.

MERS-CoV-like virus is widely distributed. What does this mean? Can camel viruses infect humans?

Camels in parts of Africa and middle east were studied

MERS-CoV-Transmission- updated Hypothesis

Roles of Camels in the transmission of MERS-CoV * _______ Camels are the only species of animals seroconvert to MERS-CoV * Camels under one year old have ____ seroprevalence (72%) * Older camels have ____ seroprevalence (95%) * Some camels have high titre to MERS-CoV in absence of any BCoV reactivity * Animals could be infected with different to BCoV and my be identical to MERSCoV

Dromedary, lower, high

SARS-CoV-2 replication cycle

Closely similar to Sars COV-1

Pathophysiology of SARS-CoV-2

Hiding in different organs; urogenital tract, vessels, etc.

SARS-CoV-2 variants

WHO- SARS-CoV-2 variants labelling and classification

SARS-CoV-2 and DIVA concept

Differentiate infected and vaccinated animals = DIVA DIVA does not distinguish between natural infection or vaccination

Strategies for treatment of Coronaviruses

Vaccines target weak points of virus.

Therapeutic trails for SARS-CoV-2

Different approaches to stop replication cycle of virus to control infection. Using polymerase inibitor

Bovine Coronavirus (BCoV) --> effects **________** species Well known in **____** and **____** production  Tropism: Epithelial cells of ___ and _______ tract  Persistent infections  Increased shedding in ____ and at ______  **BCoV clinical syndromes (3)** ?

bovine, milk, meat, gut, respiratory, winter, calving - Calf diarrhea (CD) -Winter dysentery (WD) -Bovine respiratory diseases complex (BRDC)

Dehydration (rehydrate animal) Diarrhea Vomiting

Four Clinical Syndromes associated with BCoV Infections

1. Enteric infections 2. Water dysentery 3. Respiratory infections 4. Bovine respiratory disease

(1) **Enteric Infections ** Calf _______ (CD), dehydration  Intestinal ____ atrophy - Effects calfs age ranging from ____ to __ wks of age

diarrhea, villous, Birth, 4

(2) **Winter dysentery (WD)** ________ diarrhea + upper ___ months to ____, _________ infection  Intestinal ______ atrophy

Bloody, 6, adult, respiratory, villous

(3) Respiratory Infections (BRDC) Calf respiratory disease __ wks to ___ months

2, 6

(4) Bovine respiratory disease __-__-month-old, complex (shipping fever feedlot cattle Cough, nasolacrimal discharge, pneumonia

6-9

All BCoV isolates belong to 1 serotype (2 subtypes) and are pneumo/enteric --> **_____________**

pneumoenteritis

**Only point mutations occur in the ___ gene of BCoV-___ vs BCoV-__ strains** Variation in strains based on ___ nucleotide in full length genome, changing ________, _________ of virus, etc.

S, E , R, one, tropism, behavior

What is this an image of?

Yellow Diarrhea Characteristic for bovine coronavirus Virus irritating mucous membrane --> destroys villi, shortens them, decreases surface area --> diarrhea (shooting out)

What is this an image of?

Winter dysentery Sudden onset of diarrhea with fresh blood in adults

Bovine coronavirus kinetics Incubation period: time from infection until onset of clinical signs Virus peaks after several days, then drops --> animal should recover, unless immunocompromised/coinfection with other pathogen.

**Infectious bronchitis virus (IBV)** * _____ known coronavirus * Discovered in **1930** in USA * Primary site of infection upper respiratory tract/ reproductive tract of **_______** --> **______ and ______** -->** Virus detection** 1. Viremia 2. Nasal secretions 3. Feces and Urine  Disease is most severe in **_____ _____** --> Other organs infected (sites of IBV persistence with periodic nasal shedding) 1. **______** (**Nephropathogenic** strains) 2. **Tissue _____** of one IBV strain altered from respiratory to kidney tissues by serial passage in the cloaca 3. Oviducts (**_____**?) 4. ________

First, chicken, Trachea, Bronchi, baby, chicks, Kidneys, tropism, eggs, Intestine

Infectious bronchitis Virus (IBV)  Sensitivity: 56°C 15 min at room temperature 2-3 days **__________** detergents destroy  Pathology:  -Acute catarrhal tracheitis, - **_______**  -Sinusitis, air sacculitis - Local **________**  Nephritis, -**_______** = inflammation of oviduct (**____ can not pass through --> issue in ___ production** !) **Stones in the ______ (_____) and _____ = another sign of IBV. ** Economic impacts of IBV infection in chicken: - Poor _____ performance and ______ due to reparatory infection - Losses in the ___ production and low ______ ____ production - Loss associated with **_____** damage in broilers and breeder chickens

common, Bronchitis, pneumonia, Salpingitis, eggs, egg, kidney, ureates, ureter, growth, mortality, egg, quality, egg, renal

Celiac acid? Newcastle?

IBV-induced clinical syndromes in chickens

skipped

Macroscopic lesions Respiratory tract  Mucus and redness (no hemorrhage) in trachea, froth in air sacs in older chickens.  In young chicks a yellow cheesy plug at the tracheal bifurcation is indicative of IB infection. Reproductive tract  Fluid yolk material may be found in the abdominal  Abnormal ovary having the misshapen follicles  Egg- Outer surface - Ridges or concretions Watery albumin Nephrotic form  Swollen, pale kidneys, with tubules and ureters distended with urates  In layers, urolithiasis is associated with certain dietary factors

Infectious bronchitis Virus (IBV) Many strains (differs in antigenic properties and virulence) - mutations and recombination  Affects young chicken from 3 weeks  Maternal antibodies control infection for 3 weeks  Suffocation, cough, sternutation, urate plug, drop of lay, clinical signs last for 10 – 14 days

Infectious Bronchitis Virus (IBV) * Group 3 coronavirus * ______ cause of economic loss in US ____ farms * _____ chickens * _____ chickens * ______

Main, poultry, Young, Broiler, Layers

IBV-infected egg defect

IBV

Clinical picture of IBV infection in chicken

Diagnosis of IBV * **Case history:** please indicate the case history according to each viral diseases * **Clinical signs**: please indicate the characteristic clinical signs per each virus * **PM lesions** : please indicate the characteristic PM lesions per each virus * **Laboratory Diagnosis**: -Sample collection: please indicate the appropriate sample per each virus -Detection of the Virus by EM: draw the morphology and structure of the virus -**Detection of viral Ag by different techniques (IF, HI, SNT etc) -Detection of the viral Abs by (ELISA, NT, FAT, etc) -Detection of the viral RNA (RT-PCR) -Sequencing of the viral genome**

IBV vaccines and vaccination strategies

Limitations of IBV vaccines

Kinetics of the IBV-induced immune response

Turkey coronavirus (TCoV)

know that it is called blueccomb disease Which virus? Which family? Swollen? Cloaca --> signs of diarrhea in birds

Reviewed this entirely Pantropic Canine coronavirus (CCoV) * Two variants (CCoV- __ and CCoV-___) A: Lung: extensive lobar ________ in the ______ and ______ lobe B: Abdominal cavity with _______ of the bowel -Enlargement of the ________ - _____: area of necrosis and hemorrhage

I, II, pneumonia, cranial, caudal, discoloration, spleen, Liver

CCoV

- C: Severe atrophy of intestinal villi - D: IHC: CCoV- Ag in the cytoplasm of intestinal villi

Read through this entirely Diagnosis and control of CCoV * Virus detection in _____ by ___ * Ag detection _____ * ____ detection rapid test * Lateral flow immunoassay * RT-____ * Control of CCoV: -Vaccines are available to protect _____ and _____ of all ages against CCoV -Vaccination should start about ___ wks of age (wait for maternal antibody to drop down).

feces, EM, ELISA, Ag, PCR, puppies, adults, 6

**Feline coronaviruses (FCoV)-Two pathotypes

1. Feline enteric coronavirus (FECoV) --> “Ubiquitous enteric biotype,” 2. Feline infectious peritonitis virus (FIPV) --> “virulent biotype” FIPV = big issue in NA

* FeCoV: commonly detected in ______ and _______ cats with a prevalence ranging from 36% to 75%. *** FeCoV: transmitted via the ____-____route and primarily infects ___________ *** Cats become ______ infected and continuously or ________ shed virus in feces * They generally remain _______ despite systemic infection, indicating that ______ FECV carriers play a key role in the epidemiology of FIPV

healthy, diarrheic, fecal, oral, enterocytes, persistently, intermittently, healthy, healthy

Feline infectious peritonitis virus (FIPV/FCoV) * FCoV: ubiquitous virus of domestic and large cats. * FCoV-infected cats either stay ______ or show only mild ______ (depends on immune system of animal). * Small proportion of FCoV-infected cats develop ? **** FCoV transmission is ____-____ via ______ trays and ______. ****FCoV infection of _______ is the key event in _____ pathogenesis * Sampling the ______, when present, is the most useful diagnostic step ***FCoV ______ ________, is considered the gold standard for diagnosis ** Fecal ____-_____ is not useful for diagnosis of FIP but for identification of FCoV shedders within a cat colony **A positive FCoV antibody test is not confirmatory of ______ (it is not a _____-test”) but absence of ______ _______ makes FIPV less likely

healthy, enteritis, feline infectious peritonitis (FIP), fecal, oral, litter, fomites, monocytes, FIP, effusion, antigen, immunostaining, RT-PCR, FIPV, FIPV, FCoV antibodies

Feline infectious peritonitis virus (FIPV/FCoV) * Two serotypes of FCoV (I and II) *** FCoV-type-1: induced higher antibody titers than FCoV type 2, and were more frequently associated with clinical signs and/or feline infectious peritonitis * Vaccine against FCoV should be based on FCoV type 1-related antigens *** Origin of FCoV type-II from the _________ between FCoV-type-__ and ______

recombination, 1, CCoV Exchange --> FCoV type 2 is the resulting hybrid.

Pathogenesis of FIPV

Lymphoid tissue --> blood --> virus replicated in MQ -> induces humoral immune response --> see chart for resulting steps.

Clinical forms of FIPV infection in cats

went through both in detail.

Effusive-wet form of FIPV

Dry form of FIPV uveitis mentioned here

Dry form of FIPV

Clinical picture of Feline infectious peritonitis virus (FIPV/FCoV)

ataxia, ascitis, fluid, eye pictures too

What can be seen in the images provided?

FIPV/FCoV Clinical picture of Feline infectious peritonitis virus (FIPV/FCoV) * Ultrasonography of young cat with FIPV showing abdominal effusion * Right lateral thoracic radiographic showing presence of effusion

Lab Diagnosis of FIPV ***Detection of viral NA by ___-____ on: - _____ samples (PBMCs) -Effusion: centrifugation of the effusion fluid - _______ samples: various body organs, liver, spleen - _____ (sensitivity??) - _______ _______ collected at PM examination **** Indirect detection of the infectious agent - ___ testing on blood samples - ____ testing on effusions - _____ testing on CSF samples ****_______: to show presence of FIPV-Ag in macrophages in an effusion of a cat with confirmed FIPV infection (macrophages: black head arrow)

***Detection of viral NA by RT-PCR on: - Blood samples (PBMCs) -Effusion: centrifugation of the effusion fluid -Tissue samples: various body organs, liver, spleen -CSF (sensitivity??) -Aqueous humor collected at PM examination **** Indirect detection of the infectious agent -Ab testing on blood samples -Ab testing on effusions -Ab testing on CSF samples ****IHC: to show presence of FIPV-Ag in macrophages in an effusion of a cat with confirmed FIPV infection (macrophages: black head arrow)

FIPV

Rivalta’s point-point-of care test *** (__ ___) Rivalta test of a FIP-aspiration, the fluid was colored by _______ ____ * Originally developed to differentiate transudate from exudate in human * (__ ___) results may not specific for FIPV: (+) was reported in ____ FIPV infected cats *** Test has high _______ predictive value (94%): (-) is useful to rule out FIPV at the point-of-care

+Ve, Methylene, blue, + Ve, none, negative

Rivalta’s point-point-of care test

FIPV/FeCoV vaccines and control measures *** I/N vaccine commercially available in the USA * Temperature-sensitive mutant of the type II FCoV strain DF2 **** Vaccine aims to induce local mucosal immune responses through the induction of IgA and cell-mediated immunity * Vaccine induces development of systemic antibodies against FCoV * Vaccine be used in FCoV antibody-positive cats * FCoV- Ab -negative kittens could benefit from vaccination, particularly if they subsequently enter an FCoV-endemic environment and thus would be at risk of developing FIP * If vaccination is to be given, the 1st dose should not be given before 16 weeks of age with a 2nd dose being given three weeks after the 1st dose.

***Coronaviruses in Swine can be divided into ?

Alphacoronavirus Deltacoronavirus

***Alphacoronavirus

* Porcine epidemic diarrhea virus (PEDV) * Transmissible gastroenteritis virus (TGEV) * Porcine Respiratory coronavirus (PRCoV)

****Deltacoronavirus

Porcine deltacoronavirus (PDCoV)

Transmissible gastroenteritis virus (TGEV) *** Mortality rate: _____ % in piglets less than ____ week old * TGEV infects _______ ______ of SI * _______ of the intestine with fluid in a thin translucent intestinal wall

100, one, villous enterocytes, Distention

TGEV

**SEM of villi infected with TGE

Pathogenesis of TGEV in pigs

Went through this entirely

Pathogenesis and host immune response to (TGEV)

* Secretory IgA: important immunity and viral clearance * IM immunization: humoral IgG- no protection * Oral immunization: develop IgA in mucosal secretions: protective * Protective IgA in colostrum * Cell mediated immunity is also important

Went through these images

Porcine Respiratory Coronavirus (PRCoV) **** PRCoV: evolved from the enteric ______ mutant (1984 EU, 1989 USA) **** A small mutation in the ____ gene responsible for the viral tropism (respiratory/enteric) * Cell tropism: _______ Epith and _____ macrophages & limited _____ tropism *** PRCoV: mild __________ diseases or _________ *** PRCV induces cross-protection against ______ (because very closely related) *** However, diagnosis of PRCV is a challenge. Both PRCV and TGE share many _______ sites, and so routine serology does not distinguish between the two viruses * Suspicion of PRCV may be based on _________ evidence in the _______ of enteric disease (difficult).

TGEV, S, respiratory, alveolar, GIT, respiratory, asymptomatic, TGEV, antigenic, serological, absence

Porcine epidemic diarrhea virus (PEDV) * First reported in US in April 2013 * not Zoonotic – No disease in humans * not a food safety concern *** not a new disease – but, new to North America * Similar to TGEV – but more challenging than TGEV ****Mortality rate up to ______% in __-__ Wks * ((Affect pigs of all ages *** More severe in ______ animals *** Transmission through _____-____ route

100, 3-5, young, fecal oral

Pathogenesis of PEDV in pigs * Thin wall of the intestine * PEDV infects enterocytes of ____ intestine and _____ causing -Intracellular viral replication destroy ______ -Severe villous _____ -Thin wall intestine * Loss of the enterocytes destroy the _______ capability of the small and large intestines - Pigs unable to extract energy and nutrients from its ____ or ____ -Pigs develop severe ______ ( ____________ , and ________)

small, cecum, enterocytes, atrophy, absorptive, feed, milk, diarrhea, malabsorption, osmotic

bluish/grey diarrhea on back of animal.

Diagnosis of PEDV *** _____ - _________ to detect the presence of viral NA in feces and oral fluids *** _____ : to detect virus association with the lesions in the small intestine ** _____ : to detect viral antibodies in the sera ** ____: to detect viral particles * Virus _______ *** Viral genome ______: to determine the genetic signature of the virus = gold standard

Real-time PCR, IHC, IFT, EM, isolation, sequencing

Detection of the three main swine coronaviruses (PEDV, TGEV, PDCoV) (multiplex-PCR)

multiplex pcr went through this.

Equine Coronavirus (ECoV) * Discovered in 1999 *** Highly contagious virus of ___ and _____ horse * ** Transmitted through exposure to contaminated ____ materials * Asymptomatic infection may occur * High morbidity but low mortality *** _____ and _____ in adult horses * May cause fever and respiratory diseases * Rapid progression may lead to death ***Causes _____ in young foals

foals, adult, fecal, Diarrhea, colic, enteritis

Go through boxed info

General properties of Arterivirdae Virion: - _________ - __- ___ nm in diameter -Smooth surface ______ with ____ like structure Genome: -______ (___) sense -Replication in ______ -Virion formed by _____ into -endoplasmic reticulum, released by exocytosis Common viral diseases: -Equine arteritis virus (EAV) -Porcine reproductive and respiratory syndrome virus (PRRSV) -Lactate dehydrogenase-elevating virus (LDV) of mice (in breeding colonies) -Simian hemorrhagic fever virus (SHFV) Viral tropism: -EAV: macrophages (monocytes) PRRSV: respiratory and reproductive tract

Sperical, 50, 70, envelope, ring, ssRNA, +, cytoplasm, budding

Arterivirdae

Genome Structure and Organization - Arterivirdae

Replication cycle of Arterivirdae

Ran through it.

Equine Arteritis Virus (EAV) **Equine arteritis virus (EAV) ** Order Nidovirales ** Family Arteriviridae ** Genus Arterivirus **Species affected: (Horses, Donkeys, Zebra, Ponies)  Isolates vary in virulence  Only one recognized serotype  Regional variations may occur  First U.S outbreak  1953, Ohio  Standardbred breeding farm  Infected stallions  10-70% become EAV carriers

Prevalence of EAV Varies by breed Standardbreds (24%) Thoroughbreds (4.5%) Warm bloods (3.6%) Quarter horses (0.6%) Likely due to management practices

****Transmission of EAV  Respiratory  Common where horses gather  Racetracks, sales, shows  Venereal  Acutely infected mares  Acutely and chronically infected stallions  Natural service and artificial insemination  In utero  Fomites  Equipment  Mechanical transmission  Humans, other animals  Virus inactivation  20-30 minutes at 56-58ºC  2 to 3 days at 37-38ºC  Up to 75 days at 4-8ºC  Semen remains infectious after freezing --> virus stays in semen forever EAV is the most important virus of equines.

EAV in mule Outbreaks not reported  Antibodies found  Little information on clinical signs  Experimental infections  Fever, depression  Serous ocular and nasal discharge  Pregnant donkey mares did not abort

Transmission cycle of EAV

EAV disease in Horses  Many infections ___________  Mares bred to _____-term carriers *** Severe infections  _______, ____  Most common in ____ *** Disease in _____  Fever, depression, anorexia, Edema *** Pregnant mares  ____  _________

asymptomatic, long, Respiratory, enteritis, foals, adults, Abortion, Stillbirth

EAV ***** Stallions --> __________ fertility ( _____ stage) Reduced sperm _____ caused by increased _____ temperature and ____ Decreased ______

Decreased, acute, quality, scrotal, edema, libido

ALL of this EAV- Post Mortem Lesions  Acute cases  Edema, congestion, hemorrhages  Fluid accumulation in body cavities  Foals  Pulmonary edema, interstitial pneumonia, splenic infarcts, enteritis  Aborting mares  Endometrial hemorrhages  Fetuses may be partially autolyzed

EAV

Pathogenesis of EAV

Went through this not numbers, just steps

Laboratory diagnosis of EAV  Virus isolation  Semen (carrier stallions)  Rabbit kidney cells  Nucleic acid detection  RT-PCR  Antigen detection  Immunohistochemistry  Histopathology  Serology  Virus neutralization  Complement fixation  Agar gel immunodiffusion *** Indirect fluorescent antibody  Fluorescent microsphere immunoassay *** ELISA  Carrier stallions can be detected by breeding to two seronegative mares

Differential Diagnosis  Equine influenza  Equine infectious anemia  African horse sickness  Hendra virus  Equine rhinitis A and B viruses  Equine adenoviruses  Equine herpesviruses 1 and 4  Purpura hemorrhagica  Berteroa incana (hoary alyssum) poisoning

Control of EAV **** MLV vaccine: valuable in ______ mares * -Identification of persistently infected stallions *** Breeding to ____-EAV Ab _____ mares is too risky * Export requirements: * -Horses should be sero- ______ **** Semen should be -Virus isolation (____) -RT-PCR (-Ve)

breeding, anti, positive, negative, -Ve

****Porcine Reproductive and Respiratory Syndrome (PRRSV) Two major PRRSV genotypes

1. European prototype 2. North American prototype

***European prototype EU-type, type 1 Lelystad virus (LV)

***North American prototype NA-type, type 2) known as VR-2332

PRRSV * Virus constantly changing (Quasispecies) * Strains LV and VR-2332 share * about 55–70% nucleotide identity at the genome level * and about 50–80% amino acid similarity **** Immunity produced by one strain does not protect against another strain * Variable expression of diseases severity **** Transmission: -Horizontal: respiratory, fecal-oral, direct contact -Vertical : trans placental, semen, artificial insemination semen

Genome structure and organization of PRRSV

Arteroviridae

Pathogenesis of PRRSV **** Primary target cells & replication of PRRSV: Porcine ____________ ________(PAM)

alveolar macrophages

Briefly ran through this

Porcine Reproductive and Respiratory Syndrome (PRRSV) * *** Virus spread by ? * Immunosuppressive * Virus survive long time in cold and wet conditions **** _____ infectious: small amount of the virus enough to infect entire herd * Persistent infection up to 200 days in some pig shedders * Easily mutated making vaccine preparation hard *** Difficulty in differentiation between the ______/_______ animals requires ___ tests

every possible ways (feces, milk, saliva, semen, blood, aerosol up to 6 miles, colostrum, intrauterine), Highly, filed, infected, DIVA

Effects of various temperatures on survival of PRRSV

****Respiratory PRRSV * _________ pneumonia: ____ discoloration and mottling, ______ texture * _______ lymph nodes: lymphadenopathy: all strain * PRRSV: infect and destroy the lung ______ * Thumping in pigs: “_______”(shallow, rapid breathing) are typical symptoms of pneumonia in swine

Interstitial, tan, rubbery, Enlarged, macrophages, thumping

PRRSV thumping (shallow, rapid breathing) in pigs

Reproductive PRRSV *** ______ * Stillbirth * Born dead pigs * Mummification * Virus shed in semen * Variable impacts on the quality of semen on infected animals

Abortion

Blue ear (edema and cyanosis) Mostly European type

Diagnosis of PRRSV ** FATS: fluorescent Ab examination of _____ ____ ***PCR: very sensitive especially in fetal materials in case of _____ * RFLP: Restriction fragment length polymorphism * Virus isolation * Sequencing of viral genome * ** ____ * ELISA * IFA: indirect IFT * VNT

frozen tissue, abortion, IHC

Control and Vaccines of PRRSV *** PRRSV vaccination: decrease titer and duration of viremia and viral shedding **** ____ vaccine (Boehringer Ingelheim) -PRRS-MLV -PRRS-ATP *** Not safe for _____ ________ females (abortion, may contaminate semen) * Recombination * Interfere with the future diagnostic assays * ****Some management control measures - _________ (all in all out) - Reduce concurrent infection -Replacement of ___ -Checking _____ for AI

MLV, naive, pregnant, Biosecurity, gilts, semen

Simian Hemorrhagic Fever Virus * Noticed in _________ imported from India in 1964 - all died * Fatal ___________ disease * Fever, facial edema, anorexia, dehydration, skin petechiae, diarrhea, and hemorrhage * _____ to ____ transmission (contaminated needles, etc) * Death in 5-25 days, mortality 100% * No ______ available

macaques, hemorrhagic, Blood, blood, vaccine

Simian Hemorrhagic Fever Virus Target cells

1. MQ 2. DC

Torovirus * Name the different strains: (4?) * Torus (Latin) refers to the circular convex molding in the form of a _________ that some columns or pilaster have at their bases

Equine torovirus, Bovine torovirus, Human torovirus, and Porcine torovirus, doughnut

****

The capsid may be bent into an open torus, conferring a biconcave disk or kidney shape on the virion (largest diameter 120-140 nm), or straight, resulting in a rod-shaped particle (dimensions 35 X 170 nm).

***

Comparison between ToV and CoV on the morphology and structure

Some common Torovirus diseases

Porcine Torovirus (PToV) * **Found at especially high rates in ______ with ______ *** First reported in the _______ in 1998 and emerged in many countries around the world * ___________ microscopy was used to detect PToV * Neutralization assays * indirect ELISA method * PCR is the most widely used diagnostic assay

piglets, diarrhea, Netherlands, Immunoelectron

3. Family: Roniviridae

Boxed part, images

Okavirus- YHD and GAV * Okaviruses have been detected only in _________ * The _______ _______ ______ (Penaeus monodon) appears to be the primary natural host of GAV and YHV but other penaeid and crustacean species are susceptible to natural and experimental infection. * Only genotypes 1 (____) and 2 (____) have been associated with disease **** ____ associated virus

crustaceans, giant tiger shrimp, YHV, GAV, gill GAV occurs commonly as a chronic infection in healthy brood stock and farmed black tiger prawns

Okavirus- YHD and GAV Yellow head Don't buy this

Week 10 - Nidovirales Coronaviridae Arterivirdae Ronivirdae Flashcards

(162 cards)