Week 10 - Recombinant proteins Flashcards Preview

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Flashcards in Week 10 - Recombinant proteins Deck (4)
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1

What is mAb?

Monoclonal antibodies - used therapeutically.

2

What is ATG?

Start codon.

3

Some commonly used promoters and their sources?

P lac - E. Coli lactose operon
P trp - E. Coli tryptophan operon
P L - bacteriophage lambda
P tac (trp-lac) = a hybrid promoter engineered from the best parts of P lac and P trp. Stronger than either parent promoter.

4

Glycosylation

Is the reaction in which a carbohydrate, i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor).