Week 3: DNA, Gene Expression and Epigenetics Flashcards
(224 cards)
Assembling subsets of the exons of a gene, which increases the number and diversity of proteins it encodes.
ALTERNATIVE SPLICING
A three-base sequence on one loop of a transfer RNA molecule that is complementary to an mRNA codon and connects the appropriate amino acid and its mRNA.
ANTICODON
The head-to-toe orientation of the two nucleotide chains of the DNA double helix.
ANTIPARALLELISM
A protein that binds a polypeptide and guides folding.
CHAPERONE PROTEIN
A part of a gene and its corresponding mRNA that encodes amino acids.
EXON
A mutation that alters a gene’s reading frame.
FRMAESHIFT MUTATION
A type of protein around which DNA coils in a regular pattern.
HISTONE
Part of a gene that is transcribed but is excised from the mRNA before translation into protein
INTRON
Pentose sugar + Nitrogenous Base
NUCLEOSIDE
Nucleoside or (Pentose sugar + Nitrogenous Base) + Phosphate Group
NUCLEOTIDE
A unit of chromatin structure consisting of DNA coiled around
an octet of histone proteins.
NUCLEOSOME
This was a quote from which scientist?
“A genetic material must carry out two jobs: duplicate
itself and control the development of the rest of the cell
in a specific way.”
Francis Crick, 1953
DNA is the Genetic Material, can be attributed to who?
Francis Crick, 1953
- Swiss physician and biochemist
- Isolated nuclei from white blood cells in pus
- Found an acid substance with nitrogen and phosphorus
o He called it nuclein (1871)
o Later, it was called nucleic acid
Friedrich Miescher, 1871
- English Physician
- Linked inheritance of inborn errors of metabolism with
the lack of particular enzymes.
Archibald Garrod, 1902
- English microbiologist
- Worked with Streptococcus pneumoniae bacteria,
which exists in two types: - Type S (Smooth) = Enclosed in a polysaccharide capsule
- Type R (Rough) = No capsule
Termed the conversion of one bacterial type into another
as transformation
Frederick Griffith, 1928
Transformation of one bacterial type to another is attributed to which Scientist?
Frederick Griffith, 1928
The virulent type of Streptococcus pneumoniae bacteria according to the experiments of Frederick Griffith in 1928
Type S (Smooth), the polysaccharide capsule defends it from the immune system of the body.
The animal used in Frederick Griffith’s experiments
A rat
- American physicians
- Treated lysed S bacteria with protease and DNase
- Only DNase prevented transformation
- Thus, DNA is the transforming principle
o Can convert type R bacteria into S
Avery, MacLeod and McCarty, 1944
Identified DNA as the transforming principle
Avery, MacLeod and McCarty, 1944
- American microbiologists
- Used E.coli bacteria infected with a virus that consisted
of a protein head surrounding DNA - Blender experiments showed that the virus transfers
DNA, not protein, into a bacterial cell
o Thus, DNA is the genetic material
Alfred Hershey and Martha Chase, 1953
They supported the findings of Francis Crick that DNA is indeed the genetic material
Alfred Hershey and Martha Chase, 1953
- Russian-American biochemist
- Identified the 5-carbon sugars ribose in 1909 and
deoxyribose in 1929 - Revealed chemical distinction between RNA and DNA
o RNA has ribose
o DNA has deoxyribose - Discovered that the three parts of a nucleotide are found
in equal proportions:
o Sugar
o Phosphate
o Base - Deduced that a nucleic acid building block must contain
one of each component
Phoebus Levine, 1909-1929