WEEK 3 - Microbiology Flashcards

1
Q

What are microorganisms?

A
  • Organisms too small to be seen with naked eye
  • Largest group of organisms
  • Occupy every conceivable environment
  • Most beneficial
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2
Q

What are the two types of microorganisms?

A
  • Cellular organisms
  • E.g. Bacteria, Archaea and Fungi, protists, helminths
  • Acellular infectious agents
  • E.g. Viruses and Prions
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3
Q

What is bacteria (and archaea)?

A
  • Prokaryotes
  • They lack internal membranes such as a nuclear membrane around their DNA
  • Human, plant, fungi, protists and helminths cells are eukaryotes because their DNA is enclosed in a nuclear membrane
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4
Q

What is nomenclature?

A
  • Have a two-part scientific name, consisting of a genus and a specific descriptive name
  • e.g., Escherichia (genus) coli (species)
  • Both words spelt out in full when first used
  • After that the genus may be abbreviated = E. coli
  • Name italicized
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5
Q

What is the structure of bacteria and archaea?

A
  • Unicellular
  • One linear or circular chromosome (nucleoid)
  • Most have a cell wall and glycocalyx
  • Everywhere sufficient H2O
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6
Q

What is diplo?

A

pairs

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7
Q

What is strepto?

A

chains

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8
Q

What is staphylo?

A

clusters

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9
Q

What is the bacterial cell wall?

A

Rigid structure outside plasma membrane to resist dehydration (shrinking) or swelling (bursting)

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10
Q

What is the two main types of bacterial cell wall?

A
  • Gram–positive
  • Gram–negative
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11
Q

What is gram-positive?

A
  • Stain purple
  • Thick peptidoglycan layer (sugars linked by amino acids)
  • Sensitive to penicillin and lysozyme
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12
Q

What is gram-negative?

A
  • Stain pink
  • Thin peptidoglycan layer
  • Additional outer membrane (phospholipids and lipopolysaccharides (endotoxins)
  • Not sensitive to penicillin and lysozyme
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13
Q

What are other bacterial features?

A

Glycocalyx

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14
Q

What is flagellum?

A

Move by flagellum (one or more)

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15
Q

What is fimbriae?

A

Communicate and stick by fimbriae (many)

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16
Q

What is sex pilus?

A

Share DNA through sex pilus

17
Q

What is reproduce rapidly?

A

binary fission: ~10 min

18
Q

What is endospore?

A

Formed within mother cell

19
Q

What are the prokaryotic requirements?

A
  • Water (80% H2O)
  • Energy source
  • Suitable pH, temperature
  • Aerobic and or anaerobic
  • Grow best in dark e.g., No Ultraviolet light
20
Q

What are viruses?

A
  • Most very small
  • No plasma membrane, cytosol or organelles
  • Require host cell to reproduce (e.g., animal or bacteria)
  • Easily spread (weeks in a droplet?)
  • Most are bacteriophages (infect / eat bacterial cells specifically)
21
Q

Viruses in extracellular state?

A

Extracellular state (virion)
- Nucleic acid (RNA or DNA)
- Protein coat (Capsid), different shapes
- Spikes attach to receptor sites on host
- Some enveloped by phospholipid membrane from previous host cell

22
Q

Viruses in intracellular state?

A

Intracellular state
- Capsid removed once in host
- Exists as a nucleic acid
- Uses host ribosomes to reproduce
- Host cell lyses and releases new viruses

23
Q

What are prions (PrP)

A
  • Infectious agents composed of a single protein
  • Mammals contain gene for amino acid sequence of PrP
  • Ingestion of prion PrP causes (a) cellular PrP to refold into (b) disease causing prion PrP
24
Q

Structure of prions (Prp)

A
  • Two stable tertiary (3-D) structures of PrP
  • Normal with -helices (cellular PrP)
  • Disease-causing with -sheets (prion PrP)
25
Q

Transmission

A
  • Portals of exit and entry
  • Body openings, surfaces
  • Break the ‘chain of infection’