week 5 Flashcards
(12 cards)
caspase 3 is used to…
trigger apoptosis in MAMMALS (along with caspase-7). In drosophila, drice & dcp-1 do this. In c. elegans, CED-3 does this
focal adhesions
membrane proteins that allow cell’s attachment to extracellular matrix
tau
microtubule stabilizing protein, regulated by caspases
what are the three actin regulators?
aip1, rac, and rab5. like tau, they’re all regulated by caspases
MMP
matrix metalloproteinases. degrade extracellular matrix, allowing cell migration
remodelling of sperm includes the following steps
- caspase in cyst
- individual complex by the head. moves from head to tail, and cytoplasm and organelles are degraded by caspase until they accumulate in the cyst bulge
- cyst bulge moves to waste bag near tail
dBruce
caspase inhibitor in sperm. active at tail, less active at head
how does caspase work in synaptic plasticity?
Ca enters through NMDA, initiates caspase-3, which removes AMPA from the membrane to decrease synaptic response
RFP tag
added to plasma containing Cas/V5, so we can observe cas
why do we freeze cell pellet on dry ice?
cell pellet contains smo/HA and cas/V5 that we transfected cells with last week. the dry ice provides shearing forces that rip the cell membrane
why do we add lysis buffer to cell after adding them to dry ice?
the dry ice provides shearing forces that rip the cell membrane. the buffer contains non-ionic detergent that will release smoHA or casV5 that was in cell
lysis buffer contains
salt, buffer, weak non-ionic detergent
