week 7

Question 1

how are antibodies generated?

Answer 1

foreign molecules, viruses, and/or bacteria enter body. when it binds to an antibody that fits it, the B cell that carries that specific antibody makes more of it

Question 2

what primary and secondary antibodies are we using

Answer 2

primary: mouse antiHA, mouse antiV5, mouse antitubulin. monoclonal
secondary: GOAT antimouse. polyclonal

Question 3

monoclonal vs polyclonal antibodies

Answer 3

monoclonal: binds to 1 antigen
polyclonal: antibodies are made from multile B cells.

Question 4

horseradish peroxidase

Answer 4

enzyme that binds to secondary antibody (goat antimouse).

Question 5

the antigens are…

Answer 5

SmoHA, CasV5

Question 6

What is normalized tubulin and Smo?

Answer 6

the condition with the highest tubulin density is 1, and everything else is relative to that.
normalized smo= smo density in a condition/normalized tubulin of that condition

Question 7

true or false: inactive caspase is not observed in imaging.

Answer 7

false. both inactive and active are observed bc they’re both tagged with V5. but active form is shorter so it will be lower.