Week 8 - Next generation sequencing (NGS)

Question 1

How was NGS developed?

Answer 1

Genome sequencing projects, tech advances, computational tools for aligning, assembling, compiling and comparing sequences

Question 2

What is the main idea used by genome sequencing tech?

Answer 2

Short sequences MANY times, overlap and align based on matching sections assemble into larger sequences then into Chromosome or genomes.

Question 3

What are Contigs and Scaffolds?

Answer 3

Contigs are long assembled sequences, while Scaffolds are contiguous, ordered contigs.

Scaffolds can then be added together to create chromosomes or entire genomes

Question 4

What is the differences between Shotgun and ordered assembly?

Answer 4

Shotgun = breaking up a DNA molecule randomly and then finding overlapping fragments to then build up a sequence
Ordered assembly = a set of overlapping cloned fragments

Question 5

Pyrosequencing

Answer 5

generates 150 bp per reaction
No electrophoresis or other type of fragment separation is needed
Faster than chain termination