What are they staining for? Flashcards by Brooke Hansen

Prussian Blue

Ferric Iron

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Turnball Blue

Ferrous Iron (Fe2+)

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Schmorl Technique

Reducing substances (melanin, argentaffin granules, and even formalin pigments)

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Fontana Masson

Argentaffin substances (melanin, argentaffin granules of carcinoid tumors, and some neurosecretory granules)

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Grimeluis

Argyrophil granules in neurosecretory tumors

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Churukian-Schenk

Argyrophil granules in neurosecretory tumors

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Gomori Methenamine Silver

Urates

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Bile Stain

presence of bilirubin in tissue and to distinguish bile pigments from other pigments found in tissue

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Von Kossa

presence of calcium in tissue

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Alzarin Red S

calcium in tissue

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Rhodanine

detection of copper in tissue, especially in liver, in Wilson disease

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Kinyoun

acid-fast mycobacteria

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Ziehl-Neelsen

acid-fast mycobacterium

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Fite

detection of mycobacterium leprae (causative organism of leprosy)

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Auramine-Rhodamine Fluorescence Technique

Mycobacterium tuberculosis and other acid-fast organisms

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Brown-Hopps Modification

gram-negative and gram-positive bacteria

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Modified Diff-Quik Giemsa

identification of H pylori

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Alcian Yellow-Toluidine Blue

detection of H pylori

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Hotchkiss-McManus PAS Reaction

demonstration of fungi

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Chromic Acid-Schiff (CAS)

identification of fungi

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Gridley

demonstration of fungi

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Grocott Methenamine-Silver Nitrate

demonstration of fungal organisms

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Microwave Methenamine-Silver Nitrate

rapid methoed most useful on cytospin prep or on frozen sections for the diagnosis of P jirovecii

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Mayer Mucicarmine and Alcian Blue

can be used for mucoid capsule of C neoformans

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Warthin Starry

demonstration of spirochetes

Dieterle Method

demonstration of spirochetes or the causative organism of legionellosis

Microwave Steiner and Steiner

demonstration of spirochetes, H pylori, or the causative organism of legionellosis

Cresyl Echt Violet Method I

identification of neurons in tissue sections, or demonstration of the loss of Nissl substance (chromatolysis)

Cresyl Echt Violet Method II

identification of neurons in tissue sections or the demonstration of loss of Nissl substance

Bodian Method

nerve fibers

Holmes Silver Nitrate

nerve fibers and neurofibrils

Bielschowsky-PAS

nerve fibers and the presence of neurofibrillary tangles and senile plaques in Alzheimer disease

Microwave Modification of Bielschowsky Method

nerve fibers and the presence of neurofibrillary tangles and senile plaques in Alzheimer disease

Sevier-Munger Modification of Bielschowsky

nerve fibers and neurofibrillary tangles and senile plaques in Alzheimer disease

Thioflavin S

neurofibrillary degeneration (neurofibrillary tangles, senile plaques, neurophil threads)

Mallory Phosphotungstic Acid Hematoxylin (PTAH)

glial fibers

Holzer Method

glial fibers and areas of gliosis

Cajal Stain

astrocytes

Weil Method

myelin

Luxol Fast Blue

myelin

Luxol Fast Blue-Cresyl Echt Violet

both myelin and Nissl substance

Luxol Fast Blue-Holmes Silver Nitrate

myelin and nerve fibrers

Luxol Fast Blue-PAS-Hematoxylin

myelin sheath, basement membranes, senile plaques, fungi, and corpora amylacea

Masson Trichrome

differentiate between collagen and smooth muscle (in tumors and identify increases in collagenous tissue in diseases such as cirrhosis of the liver)

Gomori 1-Step Trichrome

identification of increase in collagenous connective tissue fiber or the differentiation between collagen and smooth muscle

Van Gieson Picric Acid-Acid Fuschin

connective tissue

Verhoeff

pathologic changes in elastic fibers

Aldehyde Fuchsin

pathologic changes to elastic fibers

Russel Modification of the Movat Pentachrome

mucin, fibrin, elastic fibers, muscle and collagen

Gomori

reticular fibers

Gordon and Sweets

reticular fibers

Mallory PTAH

muscle cross-striation and fibrin

Periodic Acid-Methenamine Silver

delineates basement membranes

Oil Red O

neutral lipids (FAT)

Sudan Black B in Propylene Glycol

neutral lipids

Osmium Tetroxide

demonstration of fat that allows paraffin embedded tissues

Toluidine Blue

Mast cells

PAS

polysaccharides, neutral mucosubstances, and basement membranes

PAS with Diastase Digestion

glycogen

Best Carmine

glycogen

Mayer Mucicarmine

"epithelial" mucin

Alcian Blue (pH 2.5)

acid mucopolysaccharides

Alcian Blue (pH 1)

sulfated mucosubstances

Alcian Blue with Hyaluronidase

differentiation between epithelial and connective tissue mucins

Alcian Blue-PAS-Hematoxylin

differentiation between neutral and acidic mucosubstances

Muller-Mowry Colloidal Iron

carboxylated and sulfated mucopolysaccharides and glycoproteins

Alkaline Congo Red

amyloid

Crystal Violet

amyloid

Thioflavin T Fluorescent

amyloid

Feulgen

DNA

Methyl-Green-Pyronin Y

differentiate between DNA and RNA

May-Grunwald Giemsa

differentiation of cells present in hematopoietic tissue