WK14: DNA Viruses

Question 1

Naked, Icosahedral, dsDNA

Answer 1

ADENOVIRIDAE

Question 2

Most common serotypes of adenoviridae

Answer 2

1, 8, 11, 21, 35, 37, 40

Question 3

Adenovirus can cause: (3)

Answer 3

Epidemic keratoconjunctivitis
Acute hemorrhagic cystitis
Pharyngoconjunctival fever

Question 4

Causes severe, fatal ACUTE RESPIRATORY DISEASE (ARD)

Answer 4

Serotype 14

Question 5

enteric adenoviruses

Answer 5

serotype 40 and 41

Question 6

Adenoviridae is isolated in _______ and _______

Answer 6

Human embryonic kidney

continuous Epithelial cell lines

Question 7

Enveloped, Icosahedral, Linear dsDNA

Answer 7

Herpesviridae

Question 8

Amorphous integument surrounding the capsule

Answer 8

Herpesviridae

Question 9

True or False

Not all herpesviruses produce latency and lifelong persistence in their hosts

Answer 9

False (ALL)

Question 10

HSV-1 & HSV-2 belong to Genus: _____

Answer 10

Simplexvirus

Question 11

Transmission of HSV

Answer 11

Contact with contaminated secretions

Question 12

Oral herpes is caused by ____

Answer 12

HSV-2

Question 13

Transmission of HSV from infected mother

Answer 13

Neonatal herpes

Question 14

reduces the risk of neonatal herpes

Answer 14

CESAREAN OR SUPPRESSIVE ANTIVIRAL THERAPY

Question 15

leading cause of fatal sporadic encephalitis

Answer 15

HSV

Question 16

Caused by HSV-2 (neonates) & HSV-1 (older children,

adults)

Answer 16

Encephalitis

Question 17

HSV of the conjunctiva

Answer 17

Ocular Herpes

Question 18

best for detection of HSV

Answer 18

Antigen detection or Viral isolation

Question 19

gene for the enzyme B-galactosidase linked to a virus- induced promoter is inserted into baby hamster kidney cells

Answer 19

ELVIS test

Question 20

If HSV-1 is present in the cell lineA viral protein will activate the promoter resulting in __________

Answer 20

B-galactosidase expression

Question 21

Detection of HSV-1 in elvis test forms ____ color

Answer 21

blue

Question 22

Leading cause of mental retardation, deafness, intellectual impairment

Answer 22

CMV

Question 23

CMV is shed in

Answer 23

Saliva
Tears
Urine
Stool
Breast milk

Question 24

CMV is Best confirmed by Isolation of virus from: ________

Answer 24

Normally sterile body fluids

Question 25

Used for blood donor screening and diagnostic applications of CMV

Answer 25

PCR, branched DNA, Hybridization assays

Question 26

True or False: Congenital infection is best confirmed by isolation of CMV from the infant with first 3 weeks of life

Answer 26

False (2 weeks)

Question 27

Viral antigenemia test is based on immunocytochemical detection of

Answer 27

65-kilodalton (kDa) | Lower-matrix phosphoprotein (pp65)

Question 28

Infectious mononucleosis

Answer 28

EBV

Question 29

Reye Syndrome is a complication in a patient with _____

Answer 29

EBV

Question 30

Malignant disease of lymphoid tissue

Answer 30

Burkitt lymphoma (EBV)

Question 31

Incubation period of EBV

Answer 31

2 weeks - 2 months

Question 32

EBV infects circulating B lymphocytes and stimulates them to produce multiple ________

Answer 32

heterophile antibodies

Question 33

Excellent rapid screening test for heterophile antibodies

Answer 33

Paul-Bunnel Heterophile Antibody Test

Question 34

antibodies against the viral capsid antigen

Answer 34

ANTI-VCA

Question 35

IgG antibody to early antigen

Answer 35

ANTI-EA IgG

Question 36

antibody to early antigen, diffuse

Answer 36

ANTI-EA/D

Question 37

elevated in patients with | Burkitt lymphoma

Answer 37

Anti-EA/R IgG antibody level

Question 38

Transmission of VZV

Answer 38

Droplet inhalation | Direct contact with infectious lesions

Question 39

Produced at very high levels in the skin vesicles

Answer 39

Cell free virus

Question 40

Reactivation of VZV

Answer 40

Zoster = Shingles

Question 41

Vesicular lesions In unilateral dermatome pattern

Answer 41

VZV

Question 42

Confirms the diagnosis of VZV

Answer 42

Culture of fresh lesions (vesicles) | Fluorescent-labeled monoclonal antibodies

Question 43

True or False: | cytopathic changes of VZV is evident in 3-7 days

Answer 43

False (may not be evident)

Question 44

standard for diagnosis of VZV

Answer 44

PCR

Question 45

transmission of HHSV-6

Answer 45

saliva

Question 46

HHSV-6 is associated with childhood disease _____

Answer 46

Roseola

Question 47

Most sensitive and specific means of diagnosing primary HHV-6

Answer 47

PCR and Viral load testing

Question 48

receptor for HHV-7 to infect T lymphocytes

Answer 48

CD4

Question 49

Causes roseola

Answer 49

HHV 7

Question 50

Kaposi sarcoma-associated herpesvirus

Answer 50

HHV8

Question 51

HHV8 Plays a role in development of: | ________ & ________

Answer 51

Primary effusion lymphomas | Multicentric Castleman disease

Question 52

Includes types infecting the genital/nongenital mucosa, genital cutaneous surfaces, types seen in human cancers

Answer 52

Alphapapillomavirus

Question 53

Papillomas or warts caused by HPVs

Answer 53

papillomaviridae

Question 54

infect all children and young adults universally, with no | significant consequences

Answer 54

HPV 1, 2, 3, 4

Question 55

in the detection of papillomavirus Cytotechnologists & cytopathologists read Pap smears and look for ________

Answer 55

koilocytes

Question 56

Cells with perinuclear clearing accompanied by an increased density of the surrounding rim of cytoplasm

Answer 56

koilocytes

Question 57

Help detect HPV DNA in endocervical cells

Answer 57

Nucleic Acid Probes

Question 58

causes small pox

Answer 58

Variola virus

Question 59

variola virus is characterized as ________ accompanied by fever 

Answer 59

Synchronous progressive rash

Question 60

Virus was introduced into the country by rodents imported from Africa

Answer 60

Monkeypox virus

Question 61

Vesicular, pustular febrile illness

Answer 61

nonkeypox virus

Question 62

Smallest DNA virus

Answer 62

Parvoviridae

Question 63

Principal pathogen of parvoviridae

Answer 63

parvovirus B19

Question 64

Fifth disease

Answer 64

Erthema Infectiosum

Question 65

rashes give a slapped cheek appearance

Answer 65

prodromal phase

Question 66

Cause transient aplastic crisis, a self-limiting erythropoietic arrest

Answer 66

Parvovirus B19 Virema

Question 67

Parvovirus B19 Virema is characterized by decrease in ______

Answer 67

RBC production (bone marrow)

Question 68

Closely related to: Bovine parvovirus and Canine minute virus

Answer 68

P Human Bocavirus (HBoV)

Question 69

HBoV is a coinfection with _____ and ______

Answer 69

RSV & Human metapneumovirus (hMPV)