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Flashcards in Wound Bench Deck (39)
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1

Ideal way to collect a specimen from a wound

Asperate

2

Ideal way to transport a wound specimen to the lab

As soon as possible in specific transport media

3

Delay shorter than 6 hours

kept at ambient temperature

4

Delay longer than 6 hours

refrigerated

5

What is specimen collection is preferred?

Specimens aspirated with a needle and syringe are preferred to those collected on swabs

6

Common submitted specimen

1. Sterile body fluids
2. Biopsy/Tissue
3. Eye
4. Ear
5. Foreign body
6. Catheter tips
7. Wounds

7

What media should be inoculated from a wound?

1. BAP
2. CHOC
3. MAC
4. CNA
5. ANA BAP
6. PEA
7. THIO
8. Direct Smear

8

Purpose of BAP

All purpose medium, best for hemolysis, detects stalemating of Haemophilus around organisms such as Staphylococcus aureus

9

Purpose of MAC

Selective for Gram negative rods, (look for Lactose pink). (does not determine fermenter or non fermenter of glucose)

10

Purpose of CNA

Selective for Gram positive organism (do not determine hemolysis)

11

Purpose of CHOC

Enriched medium that will grow most any organism (supports fastidious organisms)

12

Purpose of ANA BAP

Prereduced, growth supplements added (enriched with Vitamin K. Hemin and cysteine)

13

Purpose of PEA

Phenyl Ethyl Alcohol Agar
Inhibits swarming of proteus

14

Purpose of THIO

Enhances growth of aerobes, facultative organisms, as well as obligate anaerobes

15

Purpose of direct smear

Very important to correlate with plate morphology

16

How does gas gangrene (myonecrosis) present on a gram stained direct smear from an infected wound

Necrotic background and gas bubbles

17

How long are anaerobic plates typically incubated before initial examinations?

2 days (48 hours)

18

How is quantitation determined for wound cultures

Quantitation is dependent on the quadrant where organisms are growing (1+ first quadrant; 2+ second quad, 3+ third quad, 4+ fourth quadrant)

19

What skin flora organisms would likely be seen in surface wound cultures

- Alpha and Gamma Strep
- Corynebacterium spp
- Coag Neg staph
- Propionibacterium spp.
- Staphylococcus saccharyolyticus
- staphylococcus epidermidis

20

Biochemical Tests Used to Identify:
- Coag Neg Staph

Gram Stain: GPC in clusters
Coagulase test: neg

21

Biochemical Tests Used to Identify:
- Corynebacterium spp

Gram Stain: GPR palisading
Catalase: pos
Nonmotile
opaque and or alpha

22

Biochemical Tests Used to Identify:
- Strep Viridian's (alpha or gamma strep; not group D)

Gram Stain: GPC in chain

23

Biochemical Tests Used to Identify:
- Propionibacterium spp.

Anaerobe

24

Most common organism in burns

Pseudomonas aeruginosa
MRSA
Enterococcus
Klebsiella

25

Conventional biochemical tests (and reactions) that would be used to identify the organisms that are in burns

Pseudomonas aeruginosa is Oxidase Positive

26

Most common organism causing Impetigo, Erysipelas, Cullulitus, Folliculitis, Furuncle, Carbuncle, etc.

Staph aureus

27

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause Impetigo, Erysipelas, Cullulitus, Folliculitis, Furuncle, Carbuncle, etc.

S. aureus:
- Cat +
- Coagulase +
- Beta hemolytic
- Furazolidone Susceptible
- Novobiocin Susceptible

28

Most common organism causing dog/cat bite infections

Pasteurella multocida/ canis, bacteroides spp., Fusobacterium, Prevotella, staph, capnocytopaga canimorsus

29

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause dog/cat bite infections

GNCB
- nonmotile
- facultatively anaerobic
- form nitrites from nitrates
- ox pos
- cat pos

30

Most common organism causing human bite infection

Eikenella corrodens,
Straptococcus anginosis, S. aureus, Fusobacterium nucleatum, prevotella melaninogenica