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Flashcards in Bloods Bench Deck (41)
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1

presence of bacteria in the blood

bacteremia

2

bacteria passing through (dental, colonoscopy)

transient bacteremia

3

bacteria seeding from the infected site to another area intermittently (seeding blood) (same organism off and on)

intermittent bacteremia

4

Bacteria coming from an intravascular source all of the time (endocarditis)

continuous bacteremia

5

bacteremia with organisms multiplying in the blood and symptoms showing

septicemia

6

Sepsis going on inside a site such as an iv catheter, endocarditis

primary sepsis

7

Sepsis going on in an external site such as strep from lungs, UTI, dental surgery, tooth abscess...

secondary sepsis

8

Clinical signs or symptoms that can be associated with septicemia

fever (i or d)
shaking
chills
malaise
tachypnea
prostration (laying down)
blood pressure (i or d)
DIC
WBC count (i or d)
lactic acidosis
C-RP (i)
procalcitonin (indicator of inflammation)

9

Indicators for obtaining blood cultures

*

10

What criteria are used in the selection of a site for collection of blood cultures

- draw opposite are that has iv
- draw below iv if have to draw from arm with iv

11

What is the number of collections and the appropriate time during the progression of a disease that a blood vulture should be drawn?

Number: one set = 1 aerobic bottle and one anaerobic bottle; no more than 3 sets in 24 hours (inoculate aerobic first)
- draw before antibiotic therapy
- space drawings an hour apart; if not, draw from different sites

12

What is the blood to medium ratio that is recommended for inoculating blood culture bottles and the rationale for this ratio

1 : 5 - 1 : 10

13

What precautions should be taken in the collection and handling of blood cultures?

*

14

What are the four macroscopic (observable) characeristics that would be evidence of growth in a conventional broth blood culture bottle?

*

15

Purposes of Sodium Polyanetholsulfonate (SPS) in blood culture bottles

anticoagulant

16

Purposes of resins in blood culture bottles

neutralizes antibiotics

17

What types of organisms may be better isolated from a Lysis Centrifugation (Isolator) System?

intracellular pathogens
- fungi
- yeasts
- molds

18

What is the rationale for using the Lysis Centrifugation (Isolator) System?

saphonin lysis white blood cells

19

What media and biochemicals should be used when blood culture is positive for:
- GPCs

BAP
CHOC
ANA BAP

20

What media and biochemicals should be used when blood culture is positive for:
- GPRs

BAP
CHOC
ANA BAP
(Lacto) TJ
(Bacillus) PEA
(Coryne) First three tubes only

21

What media and biochemicals should be used when blood culture is positive for:
- GNRs

BAP
CHOC
MAC
CNA
ANA BAP
- KIA FROM BLOOD or Vitek

22

What media and biochemicals should be used when blood culture is positive for:
- GNCs

BAP
CHOC
ANA BAP
ML

23

What media and biochemicals should be used when blood culture is positive for:
- yeast (YLF)

BAP
CHOC
ANA BAP
CSAB
CAC

24

What do you do if a blood culture bottle is signaled as positive by the BACTEC but no organisms are seen on the gram stained smear ("false positive")

Negative GS and acridine orang stain:
1. Subculture vial medium to CHOC and ANA BAP and incubate
2. Return Vial to instrument

25

Length of incubation and intervals of subculture for isolation of Brucella

- Held for 14 days in the Bactec FX
- subcultured weekly to trypticase soy agar; these plates are held for four days in CO2 incubator
- If sent in an ISOLATOR tube, it is plated to a BAP, CHOC , and TSA plate and held for 14days

26

Length of incubation and intervals of subculture for isolation of Bartonella

vials are held for 30 days in the BACTEC FX

27

How long are NEGATIVE blood bottles held on the BACTEC?

5 days

28

What are the 3 most common agents of subacute bacterial endocarditis (SBE)

- CoNS
- Viridans strep
- (staph aureus)

29

What are the most common GPC and GPR contaminants in blood cultures?

- alpha/gama strep (viridans)
- CoNS
- Proprionibacterium acnes
- Corynebacterium

30

Principle of the PNA/Quick FISH testing

*