Yeast/C. Elegans Flashcards
(30 cards)
replicative lifespan
number of cell divisions a mother cell can undergo
what indicates replicative age?
of bud scars
how does magnetic sorting work
biotin binds to cell wall
wash + add to new culture
stravidin binds to biotin coated iron beads
pour out + expose to magnet
why does magnetic sorting work (yeast)
daughters do not inherit their cell walls from their mothers
what contributes to replicative aging
asymmetric distribution
mito-ROGFP
reports relative oxidation in the matrix
how to report relative oxidation
look at the ratio of 2 excitation maxes
which type of mitochondria are delivered to the bud
higher quality, less oxidized
what enables normal daughter replicative lifespan
asymmetric distribution of aging factors
ERCS (Extrachromosomal rDNA) …
titrates essential rep factors
indicated genome instability
chronological lifespan
length of time that a non dividing yeast cell survives
mutations in TOR (kinase)
increase chronological lifespan of yeast
first male then female
c. elegans
which tissue do c. elegans model aging in
non-mitotic
do c. elegans replace their cells
no
when are somatic cell divisions complete in c. elegans
larval development
cameleon
genetic tool for measuring change in Ca2+
brainbow
neuron color coding, can pair w/ Ca2+ activity
how to induce a random change in dna
chemical
how to induce site-directed change in dna
crispr
guide RNA (crispr)
matches piece of DNA to modify
CAS9
cuts DNA
RNAI functional knockout
dsRNA produced in vitro induces degradation of corresponding mRNA
phenocopy
looks like phenotype of a gene mutation, but only the transcript is degraded
