02 LAB: HEMOGLOBIN DETERMINATION

Question 1

The primary function of hemoglobin within the RBC is to ____

Answer 1

carry oxygen to and from the tissues

Question 2

Hemoglobin is measured ___ or measured indirectly by converting it to compounds like ___, ___, ___, and ___

Answer 2

oxyhemoglobin
cyanmethemoglobin
acid hematin
alkali hematin
carboxyhemoglobin

Question 3

Cyanmethemoglobin is also known as ___

Answer 3

Hemoglobin cyanide (HiCN method)

Question 4

The reference method of hemoglobin measure according to CLSI

Answer 4

Cyanmethemoglobin method

Question 5

The pigment in ___ is stable even in diluted solution

Answer 5

cyanmethemoglobin

Question 6

T/F: cyanmethemoglobin standard is commercially available

Answer 6

T

Question 7

Cyanmethemoglobin method measures all forms of hemoglobin except ___

Answer 7

sulfhemoglobin

Question 8

The wavelength required for cyanmethemoglobin method

Answer 8

540 nm

Question 9

In cyanmethemoglobin method, the blood is diluted in ___

Answer 9

alkaline drabkin solution

Question 10

Components of Drabkin solution

Answer 10

Potassium ferricyanide
Potassium cyanide
Sodium bicarbonate
Surfactant (detergent)

Question 11

This component of the Drabkin solution enhances the lysis of RBCs to free the hemoglobin

Answer 11

detergent

Question 12

This component of the Drabkin solution decreases the turbidity caused by protein precipitates

Answer 12

detergent

Question 13

In the cyanmethemoglobin method, Fe2+ (hemoglobin) is oxidized to Fe3+ (methemoglobin) by the ___

Answer 13

potassium ferricyanide

Question 14

The ___ in Drabkin solution converts the methemoglobin to cyanmethemoglobin

Answer 14

potassium cyanide

Question 15

The ___ in Drabkin solution provides stability in pH and enhances the conversion within 10 minutes

Answer 15

sodium bicarbonate

Question 16

The ___ in Drabkin reagent enhances the conversion to 3 minutes

Answer 16

dihydrogen potassium phosphate

Question 17

Reference range of hemoglobin in males

Answer 17

14-18 g/dL

Question 18

Reference range of hemoglobin in females

Answer 18

12-15 g/dL

Question 19

Reference range of hemoglobin in neonates

Answer 19

16.5-21.5 g/dL

Question 20

Cyanmethemoglobin should be stored in ___ at ___ because it is ___

Answer 20

brown bottle
room temperature
sensitive to light

Question 21

Another method that has been used in some automated instruments involves the use of ___ to convert hemoglobin to methemoglobin

Answer 21

Sodium lauryl sulfate

Question 22

Carboxyhemoglobin takes ___ to convert to cyanmethemoglobin and could cause erroneous results in specimens from ___

Answer 22

1 hour
heavy smokers

Question 23

When there is a high PLT count that causes a falsely elevated hemoglobin, what should be done?

Answer 23

reagent-specimen solution should be centrifuged and the supernatant measured

Question 24

When there is lipemia that causes a falsely elevated hemoglobin, what should be the remedy?

Answer 24

Using a sample blank (0.01 mL plasma + 5 mL of cyanmethemoglobin reagent)

Question 25

When there is a presence of Hemoglobin S and Hemoglobin C that causes a falsely elevated hemoglobin, what should be the remedy

Answer 25

Make a 1:2 dilution with distilled water (1 part specimen and 1 part water) then multiply the result by 2

Question 26

When there is abnormal globulins that causes a falsely elevated hemoglobin, what should be done?

Answer 26

add 0.1g of potassium carbonate to the cyanmethemoglobin reagent

Question 27

The acid hematin method involves the conversion of hemoglobin to acid hematin by using ___, wherein the yellowish-brown solution is compared to the color standard in the ___

Answer 27

0.1 N HCl comparator black

Question 28

In the acid hematin method, the reading of the ___ meniscus is reported

Answer 28

lower

Question 29

The acid hematin method is also called as the "__"

Answer 29

Sali's acid hematin method

Question 30

The alkali hematin method uses an alkaline solution, particularly ___ for hemoglobin determination, that produces a true and relatively stable solution of hematin

Answer 30

NaOH

Question 31

One of the disadvantages of the alkali hematin method is that the ___ is alkali-resistant

Answer 31

hemoglobin F

Question 32

The ___ method of hemoglobin determination is based on the principle of color compariso

Answer 32

tallquist or scale

Question 33

The ___ method of hemoglobin determination uses simple chart consisting of different degrees of redness to serve as a comparator

Answer 33

tallquist or scale

Question 34

In the tallquist method, hemoglobin is reported in terms of ___

Answer 34

percentage

Question 35

The tallquist or scale method has a percentage error of ___

Answer 35

30-50%

Question 36

Other method of hemoglobin determination: A photometric determination of hemoglobin by measuring oxyhemoglobin

Answer 36

Sodium carbonate

Question 37

Other method of hemoglobin determination: Simple and quick procedure but there is no possibility of preparing a stable oxyhemoglobin standard

Answer 37

Sodium carbonate

Question 38

Other method of hemoglobin determination: Plus oxygen saturation as well as pulse rate is measured through the finger by attaching the photoelectric oxyhemoglobin monitor

Answer 38

Photoelectric oxyhemoglobin

Question 39

Other method of hemoglobin determination: Not done as a routine examination

Answer 39

Carboxyhemoglobin

Question 40

Other method of hemoglobin determination: Only performed when carbon monoxide poisoning is suspected

Answer 40

Carboxyhemoglobin

Question 41

Other method of hemoglobin determination: Measures the amount of oxygen using a manometric apparatus

Answer 41

Van Slyke Oxygen Capacity

Question 42

In the Van Slyke Oxygen Capacity method, the level of hemoglobin is determined by ___

Answer 42

computation

Question 43

1 gram of hemoglobin is equal to ___ O2

Answer 43

1.34 mL

Question 44

Other method of hemoglobin determination: Measures the amount of iron present

Answer 44

Kennedy's method; Wong's method

Question 45

Other method of hemoglobin determination: Based on the fact that most of the iron is found in the red blood cell and combined with the hemoglobin molecule

Answer 45

Kennedy's method; Wong's method

Question 46

1 gram of hemoglobin is equal to ___ Fe2+

Answer 46

3.47 mg

Question 47

The copper sulfate method of hemoglobin determination is specific for ___

Answer 47

blood donors

Question 48

The specific gravity of blood

Answer 48

1.053

Question 49

Distance of the drop of blood to the solution in the copper sulfate method

Answer 49

1 cm

Question 50

In the copper sulfate method, the acceptable drop of blood should sink within ___

Answer 50

15 seconds

Question 51

In the copper sulfate method, if the drop of blood sank within 15 seconds, it has a hemoglobin measure of ___

Answer 51

>12.5 g/dL

Question 52

This hemoglobin determination technique is used to detect abnormal hemoglobin or hemoglobinopathies

Answer 52

hemoglobin electrophoresis

Question 53

In hemoglobin electrophoresis, the pH of cellulose acetate is ___

Answer 53

8.2 to 8.6

Question 54

In hemoglobin electrophoresis, the pH of citrate agar is ___

Answer 54

6.2

Question 55

Cellulose acetate: "crawl"

Answer 55

A2, C, E, O

Question 56

Cellulose acetate: "slow"

Answer 56

S, D, G

Question 57

Cellulose acetate: "fast"

Answer 57

F

Question 58

Cellulose acetate: "accelerate"

Answer 58

A

Question 59

Cellulose acetate: At the anode end

Answer 59

Bart's

Question 60

Cellulose acetate: Passes through the anode end

Answer 60

Hemoglobin H

Question 61

Citrate agar: At the cathode end

Answer 61

F

Question 62

Citrate agar: At the origin

Answer 62

O, D, G

Question 63

Citrate agar: At the anode end

Answer 63

C

Question 64

Citrate agar: Between the cathode and the origin

Answer 64

A, A2, E

Question 65

Citrate agar: Between the origin and the anode

Answer 65

S