02 LAB: RED BLOOD CELL COUNT

Question 1

RBC Count is the number of RBCs in ___ of blood

Answer 1

1 L

Question 2

In traditional units, RBC count is measured by ___

Answer 2

number of cells per cubic millimeter

Question 3

Preferred tube for RBC count

Answer 3

EDTA

Question 4

Characteristics of RBC diluting fluid

Answer 4

isotonic
high specific gravity
easy to prepare
cheap
good preservative
has a buffer action
not initiate growth of molds

Question 5

The best RBC diluting fluid wherein the cell shape is not altered

Answer 5

Dacie’s/Formol Citrate

Question 6

This RBC diluting fluid is disadvantageous as it allows growth of molds

Answer 6

Hayem’s

Question 7

This RBC diluting fluid is disadvantageous as it produces clumping of cells

Answer 7

Hayem’s

Question 8

T/F: Both Dacie’s and Hayem’s can be stored for long periods of time

Answer 8

T

Question 9

T/F: Hayem’s has a corrosive effect

Answer 9

F

Question 10

This RBC diluting fluid prevents rouleaux formation

Answer 10

Gower’s

Question 11

This RBC diluting fluid precipitates protein in hemoglobinemia and hyperglobulinemia

Answer 11

Gower’s

Question 12

This RBC diluting fluid is best for beginners

Answer 12

Toisson’s

Question 12

This RBC diluting fluid is a stable preservative

Answer 12

NSS

Question 12

RBC count is multiplied by ___ to calculate the total RBC

Answer 12

10 000

Question 12

Toisson’s RBC diluting fluid supports the growth of fungi. What is the best thing to do to prevent this?

Answer 12

Filter the diluting fluid

Question 12

This RBC diluting fluid has a high specific gravity and can stain WBC

Answer 12

Toisson’s

Question 12

The RBC count in the 5 squares should not have a difference of ___

Answer 12

> 20

Question 12

This component of Toisson’s diluting fluid aid in WBC and RBC differentiation

Answer 12

Methyl violet

Question 13

This RBC diluting fluid is used in emergency cases and excessive rouleaux formation

Answer 13

NSS

Question 13

This RBC diluting fluid is used for autoagglutinated cells

Answer 13

NSS

Question 13

Reference range of RBC in males

Answer 13

4.5-6.0 M/cumm

Question 14

Reference range of RBC in female

Answer 14

4.0-5.5 M/cumm

Question 14

Reference range of RBC in late pregnancy

Answer 14

3.0-5.0 M/cumm

Question 15

Reference range of RBC at birth

Answer 15

7.0 M/cumm

Question 16

To calculate for the SI unit of RBC, the conventional unit is multiplied by ____

Answer 16

0.000001

Question 17

Overcharging in RBC count can produce a falsely ___ result

Answer 17

decreased

Question 18

How do we troubleshoot when RBC is markedly increased?

Answer 18

decrease the volume of aspirated blood

Question 19

How do we troubleshoot when RBC is markedly decreased?

Answer 19

increase the volume of aspirated blood use standard blood volume but increase the number of tertiary squares to count

Question 20

Formula for RBC count

Answer 20

Total no. of cell counted/ (1/5 x 1/10 x 1/200) Total no. cell counted / (number of squares x depth x dilution)

Question 21

Men have ___ RBC counts that women

Answer 21

higher

Question 22

Adults have ___ RBC counts than newborns

Answer 22

lower

Question 23

Dehydration and strenuous activities may result to ___ RBC count

Answer 23

increased

Question 24

It is expected to have a/n ___ RBC count in individuals living at higher altitudes

Answer 24

increased

Question 25

Pathologic variation in RBC count

Answer 25

Polycythemia Anemia/Oligocythemia Hemorrhage Drugs Secondary to other disorders/diseases

Question 26

A convenient RBC counting method wherein the dilution fluid is already pre-measured

Answer 26

Unopette system

Question 27

These significantly replaced the manual method of RBC counting

Answer 27

Automated analyzers

Question 27

Principle of automated analyzers for RBC counting

Answer 27

Electronic impedance / Flow cytometry

Question 27

In the photoelectric method of RBC counting, the ___ is proportional to the number of RBC present

Answer 27

solution turbidity

Question 28

Automated method of RBC counting: analyzes and reports CBC, RBC indices, RDW, platelet count, MVP, PDW, PLCR, and nucleated cells according to size

Answer 28

Sysmex E-5000

Question 29

Automated method of RBC counting: performs CBC, platelet count, 5-part WBC differential count including the percentages and absolute values of neutrophils, lymphocytes, monocytes, eosinophils, and basophils and calculates RBC indices, RDW, MVP, PDW, PLCR

Answer 29

Sysmex NE-8000

Question 30

Automated method of RBC counting: A combination of different hematology instruments capable of performing CBC, RBC indices, RDW, platelet count, 5-part differential, reticulocyte count, preparation of Wedge blood smear, and identification of bar coded blood specimen

Answer 30

Sysmex total hematology system (HS)

Question 31

Automated method of RBC counting: the first model of Coulter Counter Analyzers; performs CBC, MCV, MCH, MCHC, platelet count

Answer 31

Coulter Counter Model S

Question 32

Automated method of RBC counting: performs all tests analyzed in the Coulter Counter S plus RDW

Answer 32

Coulter Counter Model S Plus

Question 33

Automated method of RBC counting: this analyzer is capable of determining Histogram differential aside from the tests performed by the previous models

Answer 33

Coulter Counter Model S Plus IV

Question 34

Automated method of RBC counting: classification of WBC using this analyzer is based on the cell's VCS (volume, conductivity, and scatter) properties

Answer 34

Counter TM STKS and Coulter MAXM

Question 35

Automated method of RBC counting: can process 90 samples per hour

Answer 35

Coulter Counter Model S Plus II

Question 36

Automated method of RBC counting: can process 115 samples per hour

Answer 36

Coulter Counter Model S Plus III

Question 37

Automated method of RBC counting: the amount of blood sample used by this analyzer is reduced to 100uL instead of 1mL of blood required by the other previous models.

Answer 37

Coulter Counter Model S Plus IV

Question 38

Automated method of RBC counting: this model performs tests and reports the same as the Coulter Counter Model S Plus IV; the major alteration is the addition of an automated cap piercer used to aspirate the speciment

Answer 38

Coulter Counter S Plus V

Question 39

Automated method of RBC counting: the innovation on the model is the addition of an autosampler which is equipped with 2 rotating sample trays that can hold 32 tubes each

Answer 39

Coulter Counter S Plus VI

Question 40

Automated method of RBC counting: the said analyzer is capable of accepting and mixing 144 specimens at one time in preparation for specimen identification, aspiration, and analysis

Answer 40

Coulter Counter S Plus STKR