1) d) Detecting Proteins using Antibodies

Question 1

Immunoassay Techniques are used to _____________________

Answer 1

Immunoassay techniques are used to detect and identify specific proteins

Question 2

What does Immunoassay Techniques use?

Answer 2

These techniques use stocks of antibodies with the same specificity, known as monoclonal antibodies

Question 3

What does the ‘label’ do?

Answer 3

The ‘label’ is often a reporter enzyme
producing a colour change when binding has occurred.

Question 4

Examples of other reporter enzymes

Answer 4

chemiluminescence, fluorescence

Question 5

What is the assays role?

Answer 5

In some cases the assay uses a specific
antigen to detect the presence of antibodies.

Question 6

Describe the process of Western Blotting?

Answer 6

Western blotting is a technique, used after SDS–PAGE electrophoresis.

The separated proteins from the gel are
transferred (blotted) onto a solid medium.

The proteins can be identified using specific antibodies that have reporter enzymes attached.

Question 7

When is Western Blotting used?

Answer 7

After SDS-Page electrophoresis