1. Intro Flashcards
(43 cards)
Nucleic acid + capsid
Nucleocapsid
Structural units of the capsid
Capsomeres
Lipid membrane around nucleocapsid in some viruses
Envelope
Complete virus particle
Virion
The capsid is mad up of
Protein
The envelope is made up of
Lipids
A virus-like proteinaceous infectious particle
Prion
Structures that replicate through conversion of other host proteins
Prion
Disease caused by prions
Spongiform encephalopathy:
- scrapie
- kuru
- Creutzfeldt-Jakob disease (CJD)
- Bovine spongiform encephalopathy (BSE) (Mad cow disease)
Steps in viral replication
Attachment Penetration Uncoating Biosynthesis Morphogenesis Release
Virus propagated in chick embryos
Yellow fever virus
Used for propagation of yellow fever virus
Chick embryos
Media used in the past for growth of viruses
Chicken embryos
Media used for growth of viruses in research
Tissue explants
3 types of cell culture
Primary cell culture
Diploid cell lines
Continuous cell lines
Cell culture derived directly from animal or human donor
Primary cell culture
Commonly used primary cell cultures
Kidney cells (rhesus monkey and rabbit)
Number of passages in primary cell cultures
1 or 2 passages
Cell culture derived from fibroblasts of animal tissues
Diploid cell line
Cell culture composed of terminally differentiated and postmitotic cells
Diploid cell line
Source of diploid cell lines
Fibroblasts of animal tissues
Number of passages in diploid cell lines
20-50 passages
A cell culture from a single cell type that can be propagated indefinitely
Continuous cell lines
Number of passages of continuous cell lines
Unlimited passages
