1d- Detecting proteins using antibodies

Question 1

Immunoassay techniques use antibodies with the same what?

Answer 1

Specificity

Question 2

What are immunoassay techniques used to detect and identify?

Answer 2

Specific proteins

Question 3

What are monoclonal antibodies?

Answer 3

Stocks of antibodies with the same specificity

Question 4

An antibody specific to a protein antigen is linked to what and why?

Answer 4

A chemical label, the label is often a reporter enzyme producing a colour change. This label allows scientists to detect when binding occurs

Question 5

What are some examples of reporter enzymes which can be used as chemical labels?

Answer 5

• chemiluminescence

- fluorescence

Question 6

What is western blotting?

Answer 6

A technique used after SDS-PAGE electrophoresis

Question 7

What happens during western blotting?

Answer 7

The separated proteins from the gel are transferred (blotted) onto a solid medium, The proteins can then be identified using specific antibodies that have reporter enzymes attached