1n- Reversible binding of phosphate and the control of confirmation

Question 1

What can the addition or removal of phosphate cause in proteins, and what is this a common form of?

Answer 1

Reversible conformational change, this is a common form of post-translational modification

Question 2

What do protein kinases do?

Answer 2

Catalyse the transfer of a phosphate group to other proteins

Question 3

What happens to the terminal phosphate of ATP?

Answer 3

It is transferred to specific R groups

Question 4

What do protein phosphatases do?

Answer 4

Catalyse the reverse reaction of a protein kinase

Question 5

What does phosphorylation bring about?

Answer 5

Conformational changes, which can affect a proteins activity

Question 6

How can the activity of many cellular proteins, such as enzymes and receptors be regulated?

Answer 6

Through phosphorylation

Question 7

Different proteins can be affected in different ways by phosphorylation, in what way are the proteins affected?

Answer 7

Some proteins are activated while others are inhibited

Question 8

What happens to a protein when a phosphate group is added?

Answer 8

Negative charges are added. Ionic interactions in the unphosphorylated protein can be disrupted and new ones created