11. endoplasmic reticulum

Question 1

Chloroplast targeting domain

Answer 1

transit peptide (N terminus)

Question 2

Mitochondria targeting domain

Answer 2

Presequence (N terminus)

Question 3

Nucleus targeting domain

Answer 3

Nuclear localisation signal

Question 4

Peroxisome targeting domain (2)

Answer 4

Peroxisome targeting signal (PTS1) - C terminus

PTS2 - N terminus

Question 5

Which chaperone helps unfolded proteins enter the chloroplast?

Answer 5

Hsp70

Question 6

Which chaperone assists protein folding in the chloroplasts?

Answer 6

Hsp60

Question 7

Is the NLS cleaved off once entered the nucleus?

Answer 7

No - so proteins can re-enter nucleus if they e exported to cytosol

Question 8

How are proteins imported into the nucleus?

Answer 8

Importin binds to the NLS and diffuses through the NPC, interacting with FG-nucleoporins.

Question 9

What happens after the importing-cargo complex is within the nucleus?

Answer 9

Ran GDP is converted to RanGTP which then binds to importin and causes a conformational change so that the cargo is released

Question 10

What is required as well as SP for targeting to the ER?

Answer 10

HDEL/KDEL

Question 11

What is required for translocation of proteins into the ER lumen in yeast?

Answer 11

SEC61