3. MT binding proteins

Question 1

Plus end microtubule binding proteins

Answer 1

EB-1

DASH ring complex

Question 2

Cross linking, stabilising or bundling proteins

Answer 2

MAP2, Tau

MAP 65

Question 3

Where do Tau and MAP2 bind to?

Answer 3

The negative surface of the lattice with a + charged tail

Question 4

Where are MAP2 and Tau found?

Answer 4

MAP2 - dendrites

Tau - dendrites and axons

Question 5

what is MAP65 important for?

Answer 5

bipolar spindle formation

Question 6

What are kinesins and dyneins each responsible for?

Answer 6

Kinesins - exocytosis

Dyneins - endocytosis

Question 7

Describe the mechanism of kinesin transport along microtubules

Answer 7

Forward motor bind beta tubulin, RELEASING ADP
Forward head binds ATP
Conformational change in neck linker causes rear head to swing forward
New forward head releases ADP, trailing head hydrolyses ATP and releases Pi

Question 8

Describe the features of a Dynein heavy chain

Answer 8

Stem - where dynactin binds
Head - containing ATPase
Stalk - protrudes from ATPase wheel

Question 9

How many protofilaments make up a singlet microtubule?

Answer 9

13

Question 10

How many protofilaments make up a singlet microtubule in neurones and worms?

Answer 10

11

15

Question 11

Where are doublet or triplet microtubules found?

Answer 11

Doublet - cilia and flagella

Triplet - basal bodies, centrioles