1.1.5 post-quiz

Question 1

Hydrogen bonds between adenine and thymine

Answer 1

2

Question 2

Hydrogen bonds between guanine & cytosine

Answer 2

3

Question 3

Adenine and guanine are …

Answer 3

Purines

Question 4

Cytosine and thymine are …

Answer 4

Pyrimidines

Question 5

DNA is read by DNA polymerase in which direction?

Answer 5

5’ to 3’

Question 6

Helicase

Answer 6

An enzyme that unwinds the double helix by breaking hydrogen bonds between nitrogenous bases

Question 7

DNA Polymerase

Answer 7

An enzyme that adds nucleotides on the complementary strand and reads in the 5’ to 3’ direction

Question 8

Primase

Answer 8

An enzyme that attaches to each (template) strand and creates the RNA primers marking where to start adding nucleotides

Question 9

Ligase

Answer 9

Joins/repairs breaks in the lagging strand (3’ to 5’) that occur during replication

Question 10

Order that DNA is analyzed

Answer 10

1) DNA extraction. 2) PCR. 3) Restriction enzyme digest. 4) Gel electrophoresis

Question 11

The genetic code in genes is found in

Answer 11

The sequence of nucleotides in a DNA molecule

Question 12

Replication fork

Answer 12

The 2 strands of DNA formed after helicase unwinds them

Question 13

Chromosome(s) in prokaryotic cells

Answer 13

Usually just 1 chromosome (haploid)

Question 14

Chromosome(s) in eukaryotic cells

Answer 14

Multiple different pairs of linear chromosomes

Question 15

RFLP

Answer 15

Restriction fragment length polymorphism

Question 16

What are RFLPs?

Answer 16

Differences in DNA sequences that are recognized after being cut in specific locations by restriction enzymes (also known as DNA fragments/DNA bands during gel electrophoresis)

Question 17

What kind of bonds are there in the DNA backbone (that are cut using restriction enzymes on a single strand)?

Answer 17

Covalent bonds

Question 18

What kind of bonds are there between base pairs?

Answer 18

Hydrogen bonds