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Mycology > LABORATORY DIAGNOSIS OF FUNGI > Flashcards

LABORATORY DIAGNOSIS OF FUNGI Flashcards

1
Q

True/false

All specimens should be transported in leakproof sterile containers and processed later.

A

FALSE

later –> ASAP

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2
Q

true/false

Anaerobic transport media or anaerobic containers should always be used for fungi

A

FALSE

should NEVER be used

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3
Q

true/false

dermatophytes are insensitive to cold temperatures.

A

false

sensitive to cold temperatures

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4
Q

true/false

If delay is expected within 2h, specimens should be stored at 4 C.

A

true

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5
Q

A requisition form must include which demographics:

A

Patient name
Age and sex
Location or address
Physician name
Specific culture site
Date and time of specimen collection
Clinical diagnosis
Any special culture request

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6
Q

true/false

Specimen must have a firmly attached label indicating the patient name, specimen type, date and time of collection

A

true

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7
Q

optimum temperature of blood and CSF samples

A

30-37 C

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8
Q

optimum temperature of dermatological specimens

A

15-30 C

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9
Q

optimum temperature for specimens other than blood, CSF samples, and dermatological samples

A

4 C

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10
Q

true/false

All work in mycology should be carried out in a certified type 1 laminar airflow BSC especially when dealing with filamentous fungi.

A

false

type 2

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11
Q

true/false

Yeasts cultures should not be handled on the bench in the same manner that bacterial cultures are routinely handled.

A

false

it can be handled on the bench

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12
Q

true/false

Screw-cap tubes of slanted media are safer to handle than petri plates.

A

true

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13
Q

true/false

Petri plates should NEVER be used if Coccidioides immitis is suspected

A

true

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14
Q

Do not make slide cultures of isolates that may be:

A

Histoplasma capsulatum

Blastomyces dermatitidis

Coccidioides immitis

Coccidioides posadasii

Paracoccidioides brasiliensis

Talaromyces marneffei

Cladophialophora bantiana

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15
Q

review table on specimen collection!

A

AYAW MO?

EDI WAG

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16
Q

it is necessary to maximize recovery of fungi in order to

A

Release fungi enclosed within cells.

Concentrate fungal material in the specimen.

Help to reduce or eliminate bacteria present in contaminated specimens because of the action of mucolytic agents

17
Q

Give three mucolytic agents

A

N-acetyl-L-cysteine

5% oxalic acid

Dithiothreitol (Sputolysin2)

18
Q

What specimen should be pretreated through: Granules should be washed & crushed; other materials
should be centrifuge at 2,000g for 10min?

A

Abscess, drainage, pus,
granules

19
Q

what specimen should be pretreated through: Lysis in Isolator tubes and then centrifugation for 30min.
at 3,000g, in a 35o fixed-angle rotor or swing bucket.?

A

blood, bone marrow

20
Q

what specimen should be pretreated through: Centrifugation at 2,000g for 10min. or membrane
filtration?

A

body fluids

21
Q

what specimen should be pretreated through: Mince; gently pushed pieces down into agar?

A

nails

22
Q

what specimen should be pretreated through: Lysis with mucolytic agents, followed by centrifugation
at 2,000g for 10min?

A

respiratory secretions (BAL, sputum)

23
Q

what specimen should be pretreated through: Mince or grind in a mortar; push pieces down into agar?

A

tissue

24
Q

what specimen should be pretreated through: Centrifugation at 2,000g for 10min?

A

urine

25
Q

An approach based on demographic information, patient history (eg, travel, occupation, etc.), predisposing factors, and merely focused on manifestations (ie, signs and symptoms).

A

clinical diagnosis

26
Q

An approach that is usually microbiologic (ie, fungal CS), serologic, and molecular approach.

A

Laboratory diagnosis

27
Q

General approaches for direct detection and identification of fungi:

A

Culture based and non-culture based

Mycology (5 decks)

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